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Main description:
Detection and analysis of DNA damage is of critical importance in a variety of biological disciplines studying apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others. Individual cells within the same tissue or in cell culture may vary in the extent of their DNA damage and, consequently, can display different re- tions to it. These differences between individual cells in the same cell popu- tion are detected using in situ approaches. In situ is a Latin term meaning "on site" or "in place." It is used to denote the processes occurring or detected in their place of origin. In mole- lar and cell biology this usually refers to undisrupted mounted cells or tissue sections. In that meaning "in situ" is used as part of the terms "in situ PCR," "in situ transcription," "in situ hybridization," "in situ end labeling," and "in situ ligation." Sometimes the "in situ" term is applied at the subcellular level to cells disrupted in the process of analysis, for example, in the detection of specific sequences in chromosomes using fluorescent in situ hybridization (FISH). Historically, the term was used primarily in methods dealing with nucleic acids.
Contents:
Part I. Labeling DNA Breaks Using Terminal Transferase (TUNEL Assay)
Labeling DNA Damage with Terminal Transferase: Applicability, Specificity, and Limitations
P. Roy Walker, Christine Carson, Julie Leblanc, and Marianna Sikorska
TUNEL Assay: An Overview of Techniques
Deryk T. Loo
Electron Microscopic Detection of DNA Damage Labeled by TUNEL
Antonio Migheli
Quantitative Differentiation of Both Free 3' OH and 5' OH DNA Ends Using Terminal Transferase-Based Labeling Combined with Transmission Electron Microscopy
Yoshinori Otsuki and Yuko Ito
Determination of Three-Dimensional Distribution of Apoptotic DNA Damage by Combination of TUNEL and Quick-Freezing and Deep-Etching Techniques
Shinichi Ohno, Takeshi Baba, Nobuo Terada, and Yasuhisa Fujii
In Situ Detection of DNA Strand Breaks in Analysis of Apoptosis by Flow- and Laser-Scanning Cytometry
Zbigniew Darzynkiewicz, Elzbieta Bedner, and Piotr Smolewski
Part II. Labeling DNA Breaks Using DNA Polymerase I or its Klenow Fragment
DNA Damage Detection Using DNA Polymerase I or its Klenow Fragment: Applicability, Specificity, Limitations
Jan Hein van Dierendonck
Labeling DNA Breaks In Situ by Klenow Enzyme
Katherine A. Wood
In Situ Nick Translation at the Electron Microscopic Level
Marc Thiry
Part III. Labeling DNA Breaks Using Ligase
In Situ DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sections: An Overview
Peter J. Hornsby and Vladimir V. Didenko
Detection of Specific Double-Strand DNA Breaks and Apoptosis In Situ Using T4 DNA Ligase
Vladimir V. Didenko
In Situ Detection of Double-Strand DNA Breaks with Terminal 5'OH Groups
Vladimir V. Didenko, Hop Ngo, and David S. Baskin
Part IV. Detection of DNA Breaks in Agarose Trapped Cells: Comet Assay and Related Techniques
The Comet Assay: Principles, Applications, and Limitations
Andrew R. Collins
The Comet Assay: An Overview of Techniques
Peggy L. Olive
Ultrasensitive Detection of DNA Damage by the Combination of the Comet and TUNEL Assays
Andrei L. Kindzelskii and Howard R. Petty
Application of FISH to Detect DNA Damage: DNA Breakage Detection-FISH (DBD-FISH)
Jose Luis Fernandez and Jaime Gosalvez
Part V. Detection of Modified Bases and AP Sites in DNA
Simultaneous In Situ Detection of DNA Fragmentation and RNA/DNA Oxidative Damage Using TUNEL Assay and Immunohistochemical Labeling for 8-Hydroxy-2'-Deoxyguanosine (8-OHdG)
Alexander E. Kalyuzhny
The In Situ Detection of Apurinic/Apyrimidinic Sites and DNA Breaks Bearing Extension Blocking Termini
Philip K. Liu, Jiankun Cui, Niki Moore, and Dongya Huang
Part VI. Indirect and General Markers of DNA Damage
Markers of Poly (ADP-Ribose) Polymerase Activity as Correlates of DNA Damage
Yinong Zhou, Shi Liang, and Lawrence R. Williams
Ultrasound Imaging of Apoptosis: DNA-Damage Effects Visualized
Gregory J. Czarnota, Michael C. Kolios, John W. Hunt, and Michael D. Sherar
p53 Induction as an Indicator of DNA Damage
Galina Selivanova
Detection of Caspases Activation In Situ by Fluorochrome-Labeled Inhibitors of Caspases (FLICA)
Zbigniew Darzynkiewicz, Elzbieta Bedner, Piotr Smolewski, Brian W. Lee, and Gary L. Johnson
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 313
Weight: 570g
Availability: Available
Subcategories: Genetics
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