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MORE ABOUT THIS BOOK
Main description:
Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. It contains state-of-the-art, brand new protocols, and provides step-by-step laboratory instructions for readily reproducible results.
Contents:
PROPOSED TABLE OF CONTENTS 1 Affinity Chromatography: History, Perspectives, Limitations and Prospects Ana Ceci lia A. Roque and Christopher R. Lowe VARIOUS MODES OF AFFINITY CHROMATOGRAPHY 2 Immobilized Metal Ion Affinity Chromatography of Native Proteins Adam Charlton and Michael Zachariou 3 Affinity Precipitation of Proteins Using Metal Chelates Ashok Kumar, Igor Yu. Galaev and Bo Mattiasson 4 Immunoaffinity Chromatography Stuart R. Gallant, Vish Koppaka and Nick Zecherle 5 Dye-ligand Chromatography Stuart R. Gallant, Vish Koppaka and Nick Zecherle 6 Purification of Proteins Using Displacement Chromatography Nihal Tugcu
AFFINITY CHROMATOGRAPHY USING PURIFICATION TAGS 7 Rationally Designed Ligands for use in Affinity Chromatography: an Artificial Protein L Ana Ceci lia A. Roque and Christopher Lowe 8 Phage display of Peptides in Ligand Selection for use in Affinity Chromatography Joanne L Casey, Andrew M. Coley and Michael Foley 9 Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein Gareth M. Forde 10 Immobilized Metal Ion Affinity Chromatography of Histidine-tagged fusion proteins Adam Charlton and Michael Zachariou 11 Methods for the Purification of HQ-Tagged Proteins Beckky Godat, Laurie Engel, Natalie Betz and Tonny M. Johnson 12 Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis-Refolding Methods. Leonard K. Pattenden and Walter G. Thomas 13 Methods for the Detection of Protein-Protein and Protein-DNA Interactions using Halo-Tag (TM) Marjeta Urh, Danette Hartzell, Jacqui Mendez and Keith Wood 14 Site-specific Cleavage of Fusion Proteins Adam Charlton 15 The Use of TAGzyme for the efficient Removal of N-terminal His-tags Jose Arnau, Conni Lauritzen, Gitte E. Petersen and John Pedersen VARIOUS APPLICATIONS OF AFFINITY CHROMATOGRAPHY. 16 Affinity Processing of Cell-containing Feeds Using Monolithic Macroporous Hydrogels, Cryogels Igor Yu. Galaev and Bo Mattiasson 17 Monolithic Bioreactors for Macromolecules Mojca Ben_ina, Katja Ben_ina, Ale_ Podgornik and Ale_ _trancar 18 Plasmid DNA Purification via the Use of a Dual Affinity Protein Gareth M. Forde 19 Affinity Chromatography of Phosphorylated Proteins Grigoriy S. Tchaga 20 Protein Separation Using Phospholipid Chromatography Tzong-Hsien Lee and Marie-Isabel Aguilar 21 Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis Neils H.H. Heegaard, Christian Schou and Jesper Ostergaard
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 356
Weight: 545g
Availability: Available
Subcategories: Genetics, Pharmacology
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