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Main description:
A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.
Contents:
Part I. Introduction
Placenta and Trophoblast: Methods and Protocols: Overview I
Michael J. Soares and Joan S. Hunt
Part II. Methods for Studying Embryo Implantation and Uterine Biology
Methodologies to Study Implantation in Mice
Kaushik Deb, Jeff Reese, and Bibhash C. Paria
Blastocyst Culture
D. Randall Armant
Isolation of Hormone Responsive Uterine Stromal Cells: An In Vitro Model for Stromal Cell Proliferation and Differentiation
Virginia Rider
Rat Decidual Cell Cultures
Yan Gu and Geula Gibori
The Immortalization of Human Endometrial Cells
Graciela Krikun, Gil Mor, and Charles Lockwood
Sheep Uterine Gland Knockout (UGKO) Model
Thomas E. Spencer and C. Allison Gray
A Baboon Model for Inducing Endometriosis
Asgerally T. Fazleabas
A Baboon Model for Simulating Pregnancy
Asgerally T. Fazleabas
The Common Marmoset Monkey as a Model for Implantation and Early Pregnancy Research
Almuth Einspanier, Kai Lieder, Ralf Einspanier, and Bettina Husen
Part III. In Vitro Trophoblast and Placental Model Systems
Mouse Trophoblast Stem Cells
Jennifer Quinn, Tilo Kunath, and Janet Rossant
Connexins and Trophoblast Cell Lineage Development
Mark Kibschull and Elke Winterhager
Rcho-1 Trophoblast Stem Cells: A Model System for Studying Trophoblast Cell Differentiation
Namita Sahgal, Lindsey N. Canham, Brent Canham, and Michael J. Soares
Bovine Trophoblast Cell Culture Systems: A Technique to Culture Bovine Trophoblast Cells Without Feeder Cells
Kazuyoshi Hashizume, Arata Shimada, Haruo Nakano, and Toru Takahashi
In Vitro Induction of Trophoblast from Human Embryonic Stem Cells
Ren-He Xu
Isolation and Culture of Term Human Trophoblast Cells
Margaret G. Petroff, Teresa A. Phillips, Hakhyun Ka, Judith L. Pace, andJoan S. Hunt
Production of Human Trophoblast Cell Lines
Guy St J. Whitley
Culture and Transfection of Human Choriocarcinoma Cells
Michael W. Wolfe
In Vitro Methods for Studying Vascularization of the Murine Allantois and Allantoic Union with the Chorion
Karen M. Downs
Part IV. Phenotypic Analysis of the Placenta
Phenotypic Analysis of the Mouse Placenta
David R. C. Natale, Maja Starovic, and James C. Cross
Phenotypic Analysis of the Rat Placenta
Rupasri Ain, Toshihiro Konno, Lindsey N. Canham, and Michael J. Soares
Analysis of the Structure of the Ruminant Placenta: Methods of Fixation, Embedding, and Antibody Localization at Light and Electron Microscope Levels
F. B. P. Wooding
Characterization of the Bovine Placenta by Cytoskeleton, Integrin Receptors, and Extracellular Matrix
Christiane D. Pfarrer
Molecular Markers for Human Placental Investigation
Berthold Huppertz
Correlative Microscopy of Ultrathin Cryosections in Placental Research
Toshihiro Takizawa and John M. Robinson
Vascular Corrosion Casting of the Uteroplacental and Fetoplacental Vasculature in Mice
Kathie J. Whiteley, Christiane D. Pfarrer, and S. Lee Adamson
Analysis of Fetal and Maternal Microvasculature in Ruminant Placentomes by Corrosion Casting
Rudolf Leiser and Christiane D. Pfarrer
Part V. Molecular Analysis and Gene Transfer Techniques
Microarray Analysis of Trophoblast Cells
Vikram Budhraja and Yoel Sadovsky
Gene Expression Microarray Data Analysis of Decidual and Placental Cell Differentiation
Sue Kong, Bruce J. Aronow, and Stuart Handwerger
Assays to Determine Allelic Usage of Gene Expression in the Placenta
Paul B. Vrana
Adenoviral-Mediated Gene Delivery to Trophoblast Cells
Bing Jiang and Carole R. Mendelson
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2014
Pages: 492
Weight: 747g
Availability: Available
Subcategories: General Issues, Obstetrics and Gynaecology
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