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Main description:
The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Protocols and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods. The next six chapters comprise detailed protocols for a range of such methods . We believe that the use of these protocols should allow the reader to establish the various methods described in his or her own laboratory. In selecting the methods to be included in this section, we have concentrated on those that, arguably, have greatest current relevance to reference clinical bacteriology laboratories; we have deliberately chosen not to give detailed protocols for certain methods, such as multilocus enzyme electrophoresis that, in our opinion, remain the preserve of specialist la- ratories and that are not currently suited for general use. We feel that the methods included in this section will find increasing use in diagnostic laboratories and that it is important that the concepts, advantages, and limitations of each are th- oughly understood by a wide range of workers in the field .
Contents:
The Impact of Molecular Methods on Clinical Bacteriology
R. C. George
Genomic DNA Digestion and Ribotyping
J. Z. Jordens
Pulsed-Field Gel Electrophoresis
M. E. Kaufmann
Plasmid Analysis
A. P. Johnson and N. Woodford
DNA Amplification
J. P. Clewley and N. Saunders
Arbitrarily Primed PCR Methods for Studying Bacterial Diseases
D. Ralph and M. McClelland
Genomic Fingerprinting by Application of rep-PCR
A. M. Ridley
Molecular Approaches to the Identification of Streptococci
R. E. McLaughlin and J. J. Ferretti
Pneumococcal Diseases
A. M. Smith and K. P. Klugman
Tuberculosis & Other Diseases Caused by Mycobacterium spp
M. Goyal and D. B. Young
Diptheria
A. Efstratiou, K. E. Engler, and A. de Zoysa
Infection Caused by Legionella spp
T. G Harrison and N.K. Fry
Haemophilus Influenzae
M. A. Herbert, D. Crook, and E. R. Moxon
Meningococcal Infection
M. C. J. Maiden
Gonorrhoea
C. A. Ison
Chancroid
S. A. Morse, D. L. Trees, and P. A. Totten
Mycoplasma/Ureaplasma Infection
C. B. Gilroy and D. T. Robinson
Salmonella Infections
E. John Threlfall, M. D. Hampton and A.M. Ridley
Cholera
T. J. Barrett and D. N. Cameron
Diagnosis and Investigation of Diarrheagenic Escherichia coli
J. P. Nataro and J. Martinez
Campylobacter Infection
J. Gibson and R. J. Owen
Helicobacter pylori
R. J. Owen and J. Gibson
Staphylococci
A. Deplano and M. Struelens
Application of Molecular Techniques to the Study of Nosocomial Infection Caused by Enterococci
T. M. Coque, P. Seetulsingh, K. V. Singh, and B. E. Murray
Molecular Approaches for the Detection and Identification of ss-Lactamases
D. J. Payne and C. J.Thomson
Biochemical and Enzyme Kinetic Applications for the Characterization of ss-Lactamases
D. J. Payne and T. H. Farmer
ss-lactam Resistance Mediated by Changes in Penicillin-Binding Proteins
C. G. Dowson and T. J. Coffey
Application of Molecular Techniques for the Study of Aminoglycoside Resistance
K. J. Shaw, F. J. Sabatelli, L. Naples, P. Mann, R. S. Hare, and G. H. Miller
Molecular Investigation of Glycopeptide Resistance in Gram-Positive Bacteria
N. Woodford and J. M. Stigter
Quinolone Resistance
J. Brown and S. G. B. Amyes
Resistance to Tetracyclines, Macrolides, Trimethoprim, and Sulfonamides
M. C. Roberts
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 696
Weight: 1007g
Availability: Available
Subcategories: Diseases and Disorders, Infectious Diseases, Microbiology
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