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Main description:
Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Contents:
Identification of New Papillomavirus Types
Ethel-Michele de Villiers, Corinna Whitley, and Karin Gunst
Identification of HPV Variants
John Cason, Jon Bible, and Christine Mant
Histochemical Analysis of Cutaneous HPV-Associated Lesions
Kiyofumi Egawa
Histological Analysis of Cervical Intraepithelial Neoplasia
Michael Babawale, Rashmi Seth, Adam Christian, Wessam Al-Utayem, Ragini Narula, and David Jenkins
Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections
Woei Ling Peh and John Doorbar
Detection and Quantitation of HPV Gene Expression Using Real-Time PCR
Rashmi Seth, John Rippin, Li Guo, and David Jenkins
Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers
Svetlana Vinokurova, Nicolas Wentzensen, and Magnus von Knebel Doeberitz
Use of Biomarkers in the Evaluation of CIN Grade and Progression of Early CIN
Jan P. A. Baak and Arnold-Jan Kruse
HPV DNA Detection and Typing in Cervical Scrapes
Peter J. F. Snijders, Adriaan J. C. van den Brule, Marcel V. Jacobs, Rene P. Pol, and Chris J. L. M. Meijer
HPV DNA Detection and Typing in Inapparent Cutaneous Infections and Premalignant Lesions
Maurits de Koning, Linda Struijk, Mariet Feltkamp, and Jan ter Schegget
Establishing HPV-Containing Keratinocyte Cell Lines From Tissue Biopsies
Margaret Anne Stanley
Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic 'Raft' Cultures
Paul F. Lambert, Michelle A. Ozbun, Asha Collins, Sigrid Holmgren, Denis Lee, and Tomomi Nakahara
Differentiation of HPV-Containing Cells Using Organotypic 'Raft' Culture or Methylcellulose
Regina Wilson and Laimonis A. Laimins
Propagation of Infectious, High-Risk HPV in Organotypic 'Raft' Culture
Margaret E. McLaughlin-Drubin and Craig Meyers
Retrovirus-Mediated GeneTransfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic 'Raft' Cultures
N. Sanjib Banerjee, Louise T. Chow, and Thomas R. Broker
The HPV Xenograft Severe Combined Immunodeficiency Mouse Model
William Bonnez
The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease
Janet L. Brandsma
Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology
Yong Liu, Hong You, and Paul L. Hermonat
Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae
Peter C. Angeletti
Analysis of the Regulation of Viral Transcription
Bernd Gloss, Mina Kalantari, and Hans-Ulrich Bernard
Analysis of HPV Transcription by RPA
Jason M. Bodily and Craig Meyers
Analysis of Regulatory Motifs Within HPV Transcripts
Sarah A. Cumming and Sheila V. Graham
Detection of HPV Transcripts by Nested RT-PCR
Christine Mant, Barbara Kell, and John Cason
Analysis of HPV DNA Replication Using Transient Transfection and Cell-Free Assays
Biing Yuan Lin, Thomas R. Broker, and Louise T. Chow
Detection and Quantitation of HPV DNA Replication by Southern Blotting and Real-Time PCR
Iain M. Morgan and Ewan R. Taylor
Analysis of E7/Rb Associations
Sandra Caldeira, Wen Dong, and Massimo Tommasino
Transformation Assays for HPV Oncoproteins
Paola Massimi and Lawrence Banks
Analysis of Adeno-Associated Virus and HPV Interaction
Paul L. Hermonat, Hong You, C. Maurizio Chiriva-Internati, and Yong Liu
In Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 Oncoproteins
Miranda Thomas and Lawrence Banks
Measuring the Induction or Inhibition of Apoptosis by HPV Proteins
Anna M. Kowalczyk, Geraldine E. Roeder, Katie Green, David J. Stephens, and Kevin Gaston
Codon Optimization of Papillomavirus G
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 514
Weight: 1650g
Availability: Available
Subcategories: Infectious Diseases, Microbiology
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