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Main description:
Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for the culturing of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed by leading practitioners to solve the difficult technical problems created by the application of the powerful bacterial genetic and molecular cloning techniques to H. pylori.
Contents:
Introduction: Medical Significance of H. pylori
Martin J. Blaser
Detection of H. pylori Infection by Biopsy Urease, Histology, and Culture
Stewart Goodwin
Serology and Urea Breath Test in the Diagnosis of H. pylori Infection
Peter R. Hawtin
The Detection of H. pylori by the Polymerase Chain Reaction
Nicholas P. Mapstone
PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples
Stephen B. Fritz and T. Ulf Westblom
In Vitro Susceptibility Testing of H. pylori, Alistair McLaren. Defined Media for H. pylori
David J. Reynolds
Studies of Respiration in H. pylori
Roger J. Miles and Huai-Tzu Chang
Use of NMR to Study H. pylori Metabolism
Peter A. Chalk, Andrew D. Roberts, Alistair A. Davison, David J. Kelly, and Peter J. White
Isolation of H. pylori Genomic DNA and Restriction Analysis
Robert J. Owen and Jane Bickley
Ribosomal RNA Gene Restriction Pattern Analysis (Ribotyping) of H. pylori
Wee Tee
Differentiation of H. pylori Strains Using PCR RFLP
Harry L. T. Mobley and Paul A. Foxall
PCR-DNA Sequence Typing
Agnes Labigne
H. pylori DNA Fingerprinting Using the Arbitrarily Primed PCR (AP-PCR) or Random Amplified Polymorphic DNA (RAPD) Method
Douglas E. Berg, Janaki Lelwala-Guruge, Engin T. Incecik, Kalpana Srivastava, and Natalia S. Akopyants
Molecular Methods for Detecting Ulcerogenic Strains of H. pylori
John C. Atherton
H. pylori DNA Transformation by Natural Competence and Electroporation
Zhongming Ge and Diane E. Taylor
Random Mutagenesis of the H. pylori Genome
Agnes Labigne
Construction of a Genomic Map of H. pylori by Pulsed-Field Gel Electrophoresis (PFGE)
Nicholas Chang, Qin Jiang, and Diane E. Taylor
Molecular Characterization of H. pylori SurfaceAntigens
Peter Doig, Paul W. O'Toole, and Trevor J. Trust
Isolation and Characterization of Porins from H. pylori
Robert E. W. Hancock and Maurice Exner
Methods for the Identification of H. pylori Host Receptors
Thomas Boren, Torkel Wadstroem, Staffan Normark, Jeffrey I. Gordon, and Per G. Falk
Host Responses to H. pylori: Molecular Analysis of Cytokine Gene Expression
Susan M. Farmery and Jean E. Crabtree
Nonprimate Animal Models of H. pylori Infection
Andrew A. McColm
The Nonhuman Primate Model for H. pylori Infection
Andre Dubois and Douglas E. Berg
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 288
Weight: 476g
Availability: Available
Subcategories: Biochemistry
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