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Main description:
Populations of the western world are now healthier and enjoying higher life expectancy than ever. They are beginning to benefit from an array of costly new therapies made possible through recent rapid advances in medical science and technology, and their demands on modern medicine are rising. Meanwhile, healthcare systems are struggling with their outdated legacy models of the m- th 20 century and are experiencing ever-increasing financial pressure from g- ernments and health insurance organizations. The equation is no longer in balance, and this predicament is forcing societies to explore new approaches to managing healthcare in the future. Since the first edition of Molecular Diagnosis of Infectious Diseases was published, we have witnessed the sequencing of the (almost) complete human genome and a shift in medical research from an emphasis on genetics to the advancement and useful application of proteomics. Bioinformatics has become the key tool for managing and analyzing the upsurge of data, and faster and more effective test methods and technologies have opened up new prospects for ind- try and academia. The tools of modern genomics and proteomics are now being utilized to specifically guide the discovery of drugs for the prevention, diagnosis, and treatment of human disease. They may also help us to find a way out of the current healthcare calamity.
Contents:
Part I. Protein-Based Identification of Antigens
Proteomic Approaches to Antigen Discovery
Karen M. Dobos, John S. Spencer, Ian M. Orme, and John T. Belisle
Immunoproteomics
Alexander Krah and Peter R. Jungblut
Immunoprecipitation and Blotting: The Visualization of Small Amounts of Antigens Using Antibodies and Lectins
Stephen Thompson
Part II. DNA-Based Identification of Antigens
Representational Difference Analysis of cDNA
Lucas D. Bowler
Microarray Data Analysis and Mining
Silvia Saviozzi, Giovanni Iazzetti, Enrico Caserta, Alessandro Guffanti, and Raffaele A. Calogero
Expression Cloning
Michael J. Lodes, Davin C. Dillon, Raymond L. Houghton, and Yasir A. W. Skeiky
Part III. Identification of Epitopes and Immunomodulatory Components
Determination of Epitopes by Mass Spectrometry
Christine Hager-Braun and Kenneth B. Tomer
Identification of T Cell Epitopes Using ELISpot and Peptide Pool Arrays
Timothy W. Tobery and Michael J. Caulfield
Virus-like Particles: A Novel Tool for the Induction and Monitoring of Both T-Helper and Cytotoxic T-Lymphocyte Activity
Ludwig Deml, Jens Wild, and Ralf Wagner
Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components
Ludwig Deml, Michael Aigner, Alexander Eckhardt, Jochen Decker, Norbert Lehn, and Wulf Schneider-Brachert
Part IV. Expression of Recombinant Proteins
High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format
Jutta Drees, Jason Smith, Frank Schafer, and Kerstin Steinert
Production of Antigens in Chlamydomonas reinhardtii: Green Microalgae as a Novel Source of Recombinant Proteins
Markus Fuhrmann
Codon-Optimized Genes that Enable Increased Heterologous Expression inMammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA
Marcus Graf, Ludwig Deml, and Ralf Wagner
Part V. Purification, Modification, and Renaturation of Recombinant Proteins
Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies)
Udo Reischl
Purification of Recombinant Proteins with High Isoelectric Points
Raffaele A. Calogero and Anna Aulicino
Refolding of Inclusion Body Proteins
Marcus Mayer and Johannes Buchner
Small-Molecule-Protein Conjugation Procedures
Stephen Thompson
Part VI. Characterization of Recombinant Proteins
Structural Characterization of Proteins and Peptides
Rainer Deutzmann
Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors
Claudia Hahnefeld, Stephan Drewianka, and Friedrich W. Herberg
Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor
Uwe Schaible, Michael Liss, Elke Prohaska, Jochen Decker, Karin Stadtherr, and Hans Wolf
Part VII. Evaluation of Recombinant Proteins in Immunological Test Systems
Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays
John E. Butler
Design and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays
Alan Warnes, Anthony R. Fooks, and John R. Stephenson
Basic Problems of Serological Laboratory Diagnosis
Walter Fierz
Molecular Diagnostics Resources on the Internet
Larry Winger
Part VIII. Recombinant Receptor Molecules
Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells
Lars Toleikis, Olaf Broders, and Stefan Dubel
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 482
Weight: 703g
Availability: Available
Subcategories: Infectious Diseases
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