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MORE ABOUT THIS BOOK
Main description:
Drugs of Abuse: Neurological Reviews and Protocols is intended to provide insightful reviews of key current topics and, particularly, state-- the-art methods for examining drug actions in their various neuroanato- cal, neurochemical, neurophysiological, neuropharmacological, and molecular perspectives. The book should prove particularly useful to n- comers (graduate students and technicians) in this field, as well as to those established scientists (neuroscientists, biochemists, and molecular biologists) intending to pursue new careers or directions in the study of drugs. The book's protocols cover a wide variety of coherent methods for gathering inf- mation on quantitative changes in proteins and mRNAs at both tissue and cel- lar levels. Inducible gene expression in striatal neurons has been a hot topic over the last decade. Alterations in gene expression for a wide range of proteins in the striatum have been investigated in response to drug administration. Altered expression of given mRNAs and their product proteins constitutes essential molecular steps in the development of neuroplasticity related to long-term addictive properties of drugs of abuse. With the multiple labeling methods that are also described in the book, gene expression can be detected in a chemically identified cell phenotype; the expression of multiple genes of interest can be detected in a single cell simultaneously. Hundreds or thousands of gene expr- sion products can today be detected in one experimental setup using the pow- ful systematic cDNA macroarray or microarray screening technology. Moreover, protocols useful in analyzing the functional roles of genes and proteins (e. g.
Contents:
Part I. Special Reviews
The Temporal Sequence of Changes in Gene Expression by Drugs of Abuse
Peter W. Kalivas, Shigenobu Toda, M. Scott Bowers, David A. Baker, and M. Behnam Ghasemzadeh
Effects of Psychomotor Stimulants on Glutamate Receptor Expression
Marina E. Wolf
Adult Neural Stem/Progenitor Cells in the Forebrain: Implications for Psychostimulant Dependence and Medication
John Q. Wang, Limin Mao, and Yuen-Sum Lau
Neuroprotective Effect of Naloxone in Inflammation-Mediated Dopaminergic Neurodegeneration: Dissociation from the Involvement of Opioid Receptors
Bin Liu and Jau-Shyong Hong
Neuropharmacology and Neurotoxicity of 3,4-Methylenedioxymethamphetamine
Gary A. Gudelsky and Bryan K. Yamamoto
Learning and Memory Mechanisms Involved in Compulsive Drug Use and Relapse
Joshua D. Berke
From Drugs of Abuse to Parkinsonism: The MPTP Mouse Model of Parkinson's Disease
Yuen-Sum Lau and Gloria E. Meredith
Part II. Detection of mRNA Expression in the Striatum
In Situ Hybridization with Isotopic Riboprobes for Detection of Striatal Neuropeptide mRNA Expression After Dopamine Stimulant Administration
Yasmin L. Hurd
Combining In Situ Hybridization with Retrograde Tracing and Immunohistochemistry for Phenotypic Characterization of Individual Neurons
Catherine Le Moine
Analysis of mRNA Expression Using Double In Situ Hybridization Labeling with Isotopic and Nonisotopic Probes
John Q. Wang
Quantification of mRNA in Neuronal Tissue by Northern Analysis
Christine L. Konradi
Analysis of Gene Expression in Striatal Tissue by Multiprobe RNase Protection Assay
Neil M. Richtand
Analysis of mRNA Expression in Striatal Tissue by Differential Display Polymerase Chain Reaction
Joshua D. Berke
Semiquantitative Real-Time PCR for Analysis of mRNA Levels
Stephen J. Walker, Travis J. Worst, and Kent E. Vrana
Application of TaqMan RT-PCR for Real-Time Semiquantitative Analysis of Gene Expression in the Striatum
Andrew D. Medhurst and Menelas N. Pangalos
Systematic Screening of Gene Expression Using a cDNA Macroarray
Travis J. Worst, Willard M. Freeman, Stephen J. Walker, and Kent E. Vrana
Part III. Detection of Protein Expression in the Striatum
Quantification of Protein in Brain Tissue by Western Immunoblot Analysis
Christine L. Konradi
Immunohistochemical and Immunocytochemical Detection of Phosphoproteins in Striatal Neurons
Limin Mao and John Q. Wang
Analysis of Protein Expression in Brain Tissue by ELISA
Steffany A. L. Bennett and David C. S. Roberts
Dopamine Receptor Binding and Quantitative Autoradiographic Study
Beth Levant
Analysis of DNA-Binding Activity in Neuronal Tissue with the Electrophoretic Mobility-Shift Assay
Christine L. Konradi
Part IV. Gene Function Analysis
Viral-Mediated Gene Transfer to Study the Behavioral Correlates of CREB Function in the Nucleus Accumbens of Rats
William A. Carlezon, Jr. and Rachael L. Neve
Generating Gene Knockout Mice for Studying Mechanisms Underlying Drug Addiction
Jianhua Zhang and Ming Xu
Antisense Approaches in Analyzing the Functional Role of Proteins in the Central Nervous System
John Q. Wang and Limin Mao
Part V. Striatal Culture Preparation
Primary Striatal Neuronal Culture
Limin Mao and John Q. Wang
Primary Rat Mesencephalic Neuron-Glia, Neuron-Enriched, Microglia-Enriched, and Astroglia-Enriched Cultures
Bin Liu and Jau-Shyong Hong
Primary Culture of Adult Neural Progenitors
Steffany A. L. Bennett and Lysanne Melanson-Drapeau
Organotypic Culture of Developing Striatum: Pharmacological Induction of Gene Expression
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 530
Weight: 771g
Availability: Available
Subcategories: Pharmacology
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