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MORE ABOUT THIS BOOK
Main description:
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise.
Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.
Feature:
Detailed description of quality control testing of vectors
Methods for determination of virus titers
Sensitive assays for testing viral DNA presence in samples
Developing new, permissive immunocompetent animal models
Characterization of specific Ad proteins (hexon, fiber, or protease/proteinase)
Procedures for microinjection, transformation assays, and NK cell-mediated cytolysis
Analysis of phylogenetics and genetic variability
Contents:
Ann E. Tollefson, Mohan Kuppuswamy, and William S. M. Wold
TABLE OF CONTENTS
Vol. II Topics: Ad proteins and RNA, lifecycle and host interactions, and phylogenetics
1. Analysis of the Efficiency of Adenovirus Transcription
Cristina Iftode and S. J. Flint
2. The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein Domains
Paul M. Loewenstein, Chao-Zhong Song and Maurice Green
3. Preparation of Soluble Extracts from Adenovirus Infected Cells for Studies of RNA Splicing
Oliver Mühlemann and Göran Akusjärvi
4. In Vitro Methods to Study RNA Interference During an Adenovirus Infection
Gunnar Andersson, Ning Xu and Göran Akusjärvi
5. Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In Situ
Eileen Bridge
6. Study of Nucleolar Localization of Adenovirus Core Proteins
David A. Matthews
7. Analysis of Adenovirus Infections in Synchronized Cells
David A. Ornelles, Robin N. Shepard and Felicia D. Goodrum
8. Co-Immunoprecipitation of Protein Complexes
Peter Yaciuk
9. Chromatin Immunoprecipitation (ChIP) to Study the Binding of Proteins to the Adenovirus Genome in Vivo
Jihong Yang and Patrick Hearing
10. Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift Assays
Pilar Perez-Romero and Michael J. Imperiale
11. Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry
Anuj Gaggar, Dmitry Shayakhmetov, and Andre Lieber
12. The Use of Cell Microinjection for the in vivo Analysis of Viral Transcriptional Regulatory Protein Domains
Maurice Green, Andrew Thorburn, Robert Kern and Paul M. Loewenstein
13. Determination of the Transforming Activities of Adenovirus Oncogenes
Michael Nevels and Thomas Dobner
14. Human Adenovirus Type 12: Crossing Species Barriers toImmortalize the Viral Genome
Walter Doerfler
15. Measurement of NK Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells
John M. Routes
16. A Flow Cytometric Assay for Analysis of NK Cell-Mediated Cytolysis of Adenovirus-Transformed Cells
Graham Bottley, Graham P. Cook, and G. Eric Blair
17. Large Scale Purification and Crystallization of Adenovirus Hexon
John J. Rux and Roger M. Burnett
18. Synthesis and Assay of Recombinant Adenovirus Protease
Joseph M. Weber
19. Assay for the Adenovirus Proteinase- Purification of the Enzyme and Synthesis of a Fluorogenic Substrate
Walter F. Mangel and William J. McGrath
20. Cofactors of the Adenovirus Proteinase: Measuring Equilibrium Dissociation Constants and Stoichiometries of Binding
Walter F. Mangel and William J. McGrath
21. Characterization of the Adenovirus Fiber Protein
Jeffrey A. Engler and Jeong Shin Hong
22. Phylogenetic Analysis of Adenovirus Sequences
Balázs Harrach and Mária Benkö
23. Assessment of Genetic Variability Among Subspecies B1 Human Adenoviruses for Molecular Epidemiology Studies
Adriana E. Kajon and Dean D. Erdman
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: February, 2007
Pages: 362
Weight: 1560g
Availability: Not available (reason unspecified)
Subcategories: Genetics, Microbiology, Oncology
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CUSTOMER REVIEWS
"...recommend it to investigators working in the area of viral-host interactions." -Doody's Book Review, Weighted Numerical Score: 70 - 3 Stars
Reviews of the First edition:
"This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them. . .a very comprehensive methods book that will be a laboratory standard for years to come."- Microbiology Today
". . .this book constitutes an excellent reference text . . .this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses."-FEBS Letters