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Main description:
In the early 1990s, almost 200 yr after Edward Jenner demonstrated the effectiveness of the smallpox vaccine, a new paradigm for vaccination emerged. The conventional method of vaccination required delivery of whole pathogens or structural subunits, but in this new approach, DNA or genetic information was administered to elicit an immunological response. Once it was observed that plasmid DNA delivered in vivo led to production of an encoded transgene (1), two ground-breaking studies demonstrated that immunological responses could be generated against antigenic transgenes via plasmid DNA delivered by DNA vaccination (as this approach is called) (2,3). The appe- ance of this new vaccination strategy coincided with advances in molecular biology, which provided new tools to study and manipulate the basic elements of an organism's genome and also could also be applied to the design and production of DNA vaccines. DNA Vaccines is a major updated and enhancement of the first edition. It reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consistent with the approach of the Methods in Molecular Medicine series, these chapters contain detailed practical procedures on the latest DNA vaccine technology. The enthusiasm for DNA vaccine technology is made clear by the number of research studies published on this topic since the mid-1990s.
Contents:
Part 1: DNA Vaccine Design
DNA Vaccine Design
Janet Brandsma
Design of Plasmid DNA Constructs for Vaccines
Donna L. Montgomery and Kristala Jones-Prather
Vaccination with Messenger RNA
Steve Pascolo
A Stress Protein-Facilitated Antigen Expression System for Plasmid DNA Vaccines
Petra Riedl, Nicolas Fissolo, Joerg Reimann, and Reinhold Schirmbeck
In Vitro Assay of Immunostimulatory Activities of Plasmid Vectors
Weiwen Jiang, Charles Rich, and David Pisetsky
Part 2: DNA Vaccine Delivery Systems
Delivery of DNA Vaccines Using Electroporation
Shawn Babiuk, Sylvia van Drunen Little-van den Hurk, and Lorne Babiuk
Needle-Free Injection of DNA Vaccines
Kanakatte Raviprakash and Kevin Porter
Needle-Free Delivery of Veterinary DNA Vaccines
Sylvia van Drunen Little-van den Hurk, Shawn Babiuk, and Lorne Babiuk
Surface-Modified Biodegradable Microspheres for DNA Vaccine Delivery
Mark Keegan and Mark Saltzman
A Dendrimer-Like DNA-Based Vector for DNA Delivery: A Viral and Non-Viral Hybrid Approach
Dan Luo, Yougen Li, Soong Ho Um, and Yen Cu
Identification of Compartments Involved in Mammalian Subcellular Trafficking Pathways by Indirect Immunofluorescence
Anne Doody and David Putnam
Part 3: DNA Vaccine Adjuvants and Activity Enhancement
Adjuvant Properties of CpG Oligonucleotides in Primates
Daniela Verthelyi
Complexes of DNA Vaccines with Cationic, Antigenic Peptides are Potent, Polyvalent CD8+ T Cell-Stimulating Agents
Petra Riedl, Joerg Reimann, and Reinhold Schirmbeck
Prime Boost Strategies in DNA Vaccines
C. Jane Dale, Scott Thomson, Robert De Rose, Charani Ranasinghe, C. Jill Medveczky, Joko Pamungkas, David B. Boyle, Ian A. Ramshaw, and Stephen J. Kent
Modifying Professional Antigen Presenting Cells to Enhance DNA Vaccine Potency
C.-F. Hung, M. Yang, and T.C. Wu
Replicase-Based DNA Vaccines for Allergy Treatment
Sandra Scheiblhofer, Richard Weiss, Maximilian Gabler, Wolfgang Leitner, and Josef Thalhamer
Part 4: DNA Vaccine Applications
Immunological Responses of Neonates and Infants to DNA Vaccines
Martha Sedegah and Stephen L. Hoffman
DNA Vaccines for Allergy Treatment
Richard Weiss, Sandra Scheiblhofer, and Josef Thalhamer
Protection From Autoimmunity by DNA Vaccination Against T Cell Receptor (TCR)
Thorsten Buch and Ari Waisman
Use of Bone Marrow-Chimeric Mice in Elucidating Immune Mechanisms
Akiko Iwasaki
Part 5: DNA Vaccine Production, Purification, and Quality
A Simple Method for the Production of Plasmid DNA in Bioreactors
K. Listner, L. Kiser Bentley, and Michael Chartrain
Practical Methods for Supercoiled pDNA Production
John Ballantyne
Production of Plasmid DNA in Industrial Quantities According to cGMP Guidelines
Joachim Schorr, Peter Moritz, Astrid Breul, and Martin Scheef
Large-Scale Non-Chromatographic Purification of Plasmid DNA
Jason Murphy and Sangeetha Sagar
Assuring the Quality, Safety and Efficacy of DNA Vaccines
James S. Robertson
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 402
Weight: 589g
Availability: Available
Subcategories: Immunology
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