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Main description:
The term "electrophoresis" was first used by Michaelis in 1909, to - scribe the migration of colloids in an electric field. The first practical elect- phoresis method was described by Tiselius in 1937. He used a U-tube filled with buffer layered on top of sample; migration could be monitored using Schlieren optics. In zone electrophoresis, the U-tube was replaced by paper, a support material employed simply to prevent or minimize diffusion of ions, so that ions applied in a narrow strip to the paper will separate and remain as relatively discrete zones. Paper was superceded by a variety of other media, - cluding cellulose acetate, hydrolyzed starch (starch gel), agarose, and polyacry- mide. The latter, in addition to being a support medium, has size-sieving properties. From the basic zone electrophoresis, other means of separation have been dev- oped. These include, isoelectric focusing, isotachophoresis, density gradient el- trophoresis, and various forms of immunoelectrophoresis. In some ways Capillary Electrophoresis (CE) has gone full circle back to the original method of Tiselius.
In its simplest form, separations occur in a buffer solution within a glass (fused silica) tube and detection occurs as sample moves past an optical window. CE has rapidly developed into a technique that rivals HPLC in its versatility. All the classical electrophoretic separations-zone, IEF, and isotachophoresis-have their counterparts in CE. Excitingly so, and - thoritatively treated in Clinical Applications of Capillary Electrophoresis.
Contents:
Clinical Applications of Capillary Electrophoresis, Margaret A. Jenkins. Serum Protein Electrophoresis, Margaret A. Jenkins. Urine Proteins, Margaret A. Jenkins. Electrophoresis of Cerebrospinal Fluid, Geoffrey Cowdrey, Maria Firth, and Gary Firth. Immunosubtraction as a Means of Typing Monoclonal and Other Proteins in Serum and Urine, Stephen M. Palfrey. Analysis and Classification of Serum Cryoglobulins, Zak K. Shihabi. Myoglobin Analysis, Zak K. Shihabi. Enzyme Analysis: Cathepsin D as an Example, Zak K. Shihabi. Quantification of Human Cytomegalovirus by Competitive PCR and Capillary Electrophoresis, Zhongxin Yu, W. Douglas Scheer, and James M. Hempe. Laboratory Diagnosis of Structural Hemoglobinopathies and Thalassemias by Capillary Isoelectric Focusing, James M. Hempe and Randall D. Craver. Serum Apolipoproteins, Layle K. Watkins, Steven L. Cockrill, and Ronald D. Macfarlane. Gene Dosage in Capillary Electrophoresis: Prenatal Diagnosis of Down's Syndrome and Rh D/d Genotyping, Pier Giorgio Righetti, Cecilia Gelfi, and Gian Franco Cossu. Rapid Analysis of Amplified Double-Stranded DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection, Ming-Sun Liu and Fu-Tai Albert Chen. Identification of Mutated p53 in Cancers by Nongel-Sieving Capillary Electrophoretic SSCP Analysis, Michiei Oto. Detection of Microsatellite Instability in Cancers by Means of Nongel-Sieving Capillary Electrophoresis, Michiei Oto. Serum Lamotrigine Analysis, Zak K. Shihabi. Acetonitrile Stacking: Serum Phenobarbital as an Example, Zak K. Shihabi. Confirmation of the Presence of Drugs of Abuse in Urine, Stephen M. Palfrey. Steroid Analysis by Micellar Electrokinetic Capillary Chromatography, Amin A. Mohammed, John R. Petersen, and Michael G. Bissell. Determination of Polyamines by Capillary Electrophoresis, Yinfa Ma, Qingnan Yu, and Bingcheng Lin. Urinary Oxalate and Citrate, Ross P. Holmes and Martha Kennedy. Plasma Nitrite and Nitrate Determination, Toshiko Ueda, TsuyoshiMaekawa, and Kazuyuki Nakamura. Index.
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2013
Pages: 212
Weight: 340g
Availability: Available
Subcategories: Pathology
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