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MORE ABOUT THIS BOOK
Main description:
During the past few decades, technical and conceptual breakthroughs have led to a virtual revolution in developmental biology. In part through cross-species compa- sons and multidisciplinary approaches (combining, for example, classical embry- ogy, genetics, molecular biology, and systems biology), major questions have often been redefined and examined from new angles and with innovative tools. Analyses using such model systems as Drosophila, Xenopus, zebrafish, chick, human, and mouse have underscored the remarkable extent to which molecular and genetic pa- ways are conserved across species and throughout embryonic, fetal, and adult dev- opment. What we learn from the embryo, then, is not only of fundamental interest, but may well have future practical applications in the clinic. A number of excellent volumes, including several in this series (e. g. , Hema- poietic Stem Cell Protocols, Klug and Jordan, eds. , 2002), have surveyed methods used in the study of hematopoiesis-the processes by which the multiple lineages of the blood form from stem and progenitor cells during ontogeny and throughout the entire life of the animal. These collections of protocols have focused largely on the postnatal cells of mouse and human. Our understanding of hematopoietic devel- ment, however, has benefitted enormously from investigations in a variety of org- isms at different stages of ontogeny.
Contents:
Part I. Genetic Approaches
Gene-Targeting and Transgenic Strategies for the Analysis of Hematopoietic Development in the Mouse
Hanna K. A. Mikkola and Stuart H. Orkin
Inducible Transgene Expression in Mouse ES Cells
David T. Ting, Michael Kyba, and George Q. Daley
Quantitative Trait Analysis in the Investigation of Function and Aging in Hematopoietic Stem Cells
Hans-Willem Snoeck
Part II. Analysis of Cell Fate Outcomes in Transplantation Models
Flow Cytometric Analysis of Hematopoietic Development
Edward F. Srour and Mervin C. Yoder
Reconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem Cells
Elisabeth H. Javazon, Aziz M. Merchant, Enrico Danzer, and Alan W. Flake
Reconstitution of Hematopoiesis Following Transplantation Into Neonatal Mice
Scott A. Johnson and Mervin C. Yoder
Part III. Animal Models
Drosophila
Hemocyte Development During Drosophila Embryogenesis
Robert A. Schulz and Nancy Fossett
Xenopus
Tracking and Programming Early Hematopoietic Cells in Xenopus Embryos
Maggie Walmsley, Aldo Ciau-Uitz, and Roger Patient
Fate Mapping Hematopoietic Lineages in the Xenopus Embryo
Mary Constance Lane and Michael D. Sheets
Analyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus laevis (J Strain)
Yumi Izutsu and Mitsugu Maeno
Use of Flow Cytometry and Combined DNA/Surface Staining for Analysis of Hematopoietic Development in the Xenopus Embryo
James B. Turpen
Zebrafish
Analysis of Hematopoietic Development in the Zebrafish
Noelle N. Paffett-Lugassy and Leonard I. Zon
Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos
Philippe Herbomel and Jean-Pierre Levraud. Chick
In Vivo Methods to Analyze CellOrigins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo
Francoise Dieterlen-Lievre, Sophie Creuzet, and Josselyne Salaun
Mouse
Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular Development
Margaret H. Baron and Deanna Mohn
Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo
Catherine Robin and Elaine Dzierzak
Hematopoietic Stem Cell Development During Mouse Embryogenesis
Julien Y. Bertrand, Sebastien Giroux, Ana Cumano, and Isabelle Godin
Analysis of Hematopoietic Progenitors in the Mouse Embryo
James Palis and Anne Koniski
In Vivo and In Vitro Assays of Thymic Organogenesis
Julie Gordon, Valerie A. Wilson, Billie A. Moore-Scott, Nancy R. Manley, and C. Clare Blackburn
Retroviral Transduction in Fetal Thymic Organ Culture
Bronwyn M. Owens, Robert G. Hawley, and Lisa M. Spain
Expansion and Differentiation of Immature Mouse and Human Hematopoietic Progenitors
Helmut Dolznig, Andrea Kolbus, Cornelia Leberbauer, Uwe Schmidt, Eva-Maria Deiner, Ernst W. Mullner, and Hartmut Beug
A New Clonal Assay System for Lymphoid and Myeloid Lineages
Hiroshi Kawamoto and Yoshimoto Katsura
Hematopoietic and Endothelial Development of Mouse Embryonic Stem Cells in Culture
Kyunghee Choi, Yun Shin Chung, and Weh Jie Zhang
Analysis of the Vascular Potential of Hematopoietic Stem Cells
Steven M. Guthrie, Sergio Caballero, Robert N. Mames, Maria B. Grant, and Edward W. Scott
Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culture
Elizabeth A.V. Jones, Margaret H. Baron, Scott E. Fraser, and Mary E. Dickinson
Fluorescent Protein-Cell Labeling and Its Application in Time-Lapse Analysis of Hematopoietic Differentiation
Matthias
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 480
Weight: 809g
Availability: Available
Subcategories: Haematology
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