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Main description:
The accurate and reliable diagnosis of transmissible diseases is the most powerful weapon available to ensure their control, and in some cases eradication. The detection of parasites in clinical cases, companion and farm animals, and in the environment is relatively easy since many of them are visible to the naked eye, and those that are not are readily detected by light microscopy. Fungal infections can similarly be determined. Bacteria are somewhat harder to detect. Although their presence can frequently be detected by light microscopy, differential diagnosis, beyond their gross morphology, is almost always impossible. However, most bacterial pathogens can be cultured in the laboratory and can be accurately identified by combinations of a series of simple tests such as morphology, staining, antibiotic sensitivity, biochemical analyses, nutrient dependence, and phage sensitivity. Viruses, however, have proved much more difficult; their size and absolute dependence of the host cell for propagation have rendered useless the methods traditionally used for other microorganisms. Until the development of tissue culture in the middle of this century, diagnosis was entirely dependent on the skill and experience of the clinician. But this was an unreliable process since many of the common virus infections exhibit similar clinical symptoms, such as coryza, exanthema, vomiting, diarrhea, neuralgia, and lethargy. Indeed many viral infections display clinical signs that are indistinguishable from bacterial or parasitic infections.
Contents:
Rapid Detection of Adenovirus from Fecal Specimens
Tanvir Tabish, Alan Warnes, and Stuart Clark
Alphaviruses
John T. Roehrig, Teresa M. Brown, Alison J. Johnson, Nick
Karabatsos, Denise A. Martin, Carl J. Mitchell, and Roger S. Nasci
Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR
Xi Jiang and David O. Matson
Enteroviruses and Rhinoviruses
Peter Muir
Rapid Detection and Identification of Dengue Viruses by Reverse Transcriptase/Polymerase Chain Reaction
Dennis W. Trent, Gwong-Jen Chang, A. Vance Vorndam,
and Robert S. Lanciotti
Hepatitis Viruses
Girish J. Kotwal
Herpesviruses
Mark J. Espy, P. Shawn Mitchell, David H. Persing, and Thomas F. Smith
Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper
Sharon Cassol, Stanley Read, Bruce G. Weniger, Richard Pilon, Barbara Leung, and Theresa Mo
PCR for the Detection of Influenza Viruses in Clinical Material
Lyssaviruses: Special Emphasis on Rabies Virus
Herve Bourhy
Methods for Detecting Anti-Measles, Mumps, and Rubella Virus Antibodies
Ilkka Julkunen, Irja Davidkin, and Christian Oker-Blom
Papillomaviruses
Sandra H. Kirk and David T. Y. Liu
Diagnosis of Parvovirus B19-DNA by Dot-Blot Hybridization
Karen E. Hicks, Stuart Beard, Bernard J. Cohen, and Jonathan P. Clewley
Polioviruses: Concurrent Serotyping and Intratypic Differentiation of Polioviruses
David J. Wood
Poxviruses
Hermann Meyer, Susan L. Ropp, and Joseph J. Esposito
Respiratory Syncytial Virus and Other Pediatric Respiratory Virus Infections
G. L. Toms
Detection and Molecular Epidemiology of Rotavirus by RNA Gel Electrophoresis
Lennart Svensson
Detection of PCR-Amplified Sandfly Fever Sicilian Virus RNAby Colorimetric Enzymatic Immunoassay
M. Sofi Ibrahim and Connie S. Schmaljohn
Time-Resolved Fluorescence
Pekka Halonen and Timo Loevgren
Enzyme-Linked Immunosorbent Assays
David M. Kemeny
Dynamic Analysis of Viral Populations by Direct DNA Sequencing and Solid-Phase Technology
Joakim Lundeberg, Jacob Odeberg, and Mathias Uhlen
Robotic Analyzers
Dale R. Pfost and Richard G. Keightley
The Detection of Enteroviruses in Water and Associated Materials Using the Polymerase Chain Reaction
Peter Wyn-Jones and Jane Sellwood
Recombinant Antigens in Viral Diagnosis
John R. Stephenson and Alan Warnes
NASBA: A Method for Nucleic Acid Diagnostics
Dianne van Strijp and Pierre van Aarle
Oligonucleotides
Stina Syrjanen
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 372
Weight: 587g
Availability: Available
Subcategories: Pathology
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