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Main description:
The era of molecular pathology has arrived. From its promising beg- nings in research laboratories, the field has grown, and continues to grow, to become a vital part of the care of an ever-increasing number of patients. Because of its recent emergence from the research taboratory, many molecuIar pathology protocols we still to be found in the primary litcramre, and have not appeared in a text. MO~PCU~Q~ Padhoiogy Protocob contains la- ratory protocoIs that have been developed by many of the authors for use in clinical molecular pathology laboratories and describe in detail Row to perform these assays. This book is therefore intended for clinical laboratory use by medical technologists and pathologists. It will dso be of interest to research workers who are performing these assays. In its broadest meaning, pathology is the study of disease, and therefore it follows that any disease for which the molecular basis is understood would be suitable as a topic for inclusion in this work. When seiecting protocols, it was necessary to place limits on the number of chapters that could be feasibly presented in a single work. Those protoculs that were selected are performed more frequently, or have achieved recognition as having important diagnostic utility in contemporary practice. A decision was made to exclude inherited genetic diseases with certain exceptions, such as those diseases that are associated with thrombotic states and are part of the traditional dumain of pathology.
Contents:
DNA Extraction from Paraffin-Embedded Tissues
Hongxin Fan and Margaret L. Gulley
DNA Extraction from Fresh or Frozen Tissues
Hongxin Fan and Margaret L. Gulley
RNA Extraction from Fresh or Frozen Tissues
Hongxin Fan and Margaret L. Gulley
Single-Strand Conformation Polymorphism Analysis of Mutations in Exons 4-8 of the TP53 Gene
Kirsi H. Vahakangas, Katariina Castren, and Judith A. Welsh
Cleavase (R) Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection
Laura Heisler and Chao-Hung Lee
Detection of Telomerase by In Situ Hybridization and by the Polymerase Chain Reaction-Based Telomerase Activity Assay
Carmela P. Morales and Shawn E. Holt
Detection of Microsatellite Instability
Karin D. Berg, Constance A. Griffin, and James R. Eshleman
Polymerase Chain Reaction Clonality Assays Based on X-Linked Genes
Langxing Pan and Huaizheng Peng
Fluorescent In Situ Hybridization: Evaluation for Ploidy and Gene Amplification
Susan Sheldon
HER-2/neu Oncogene Amplification Determined by Fluorescence In Situ Hybridization, Nested Reverse Transcription-Polymerase Chain Reaction Assay to Detect BCR/abl
Linda M. Wasserman
Detection of t(15;17)(q24;q21), inv(16)/t(16;16)(p13;q22), and t(8;21)(q22;q22) Anomalies in Acute Myeloid Leukemia
David S. Viswanatha
Detection of t(14;18)(q32;q21)-Associated BCL-2/JH Gene Fusion in Non-Hodgkin's Lymphoma
David S. Viswanatha
Detection of Breast Cancer Cells Using Immunomagnetic Beads and Reverse Transcription-Polymerase Chain Reaction
Scott Luke and Karen L. Kaul
Molecular Detection of Circulating Prostate Cancer Cells
Karen L. Kaul
Methods to Detect Clonal Gene Rearrangements in Lymphomas and Leukemias
Naheed Mitha and Ronald C. McGlennen
Monitoring of Bone Marrow TransplantEngraftment
Kristine P. Woronzoff-Dashkoff and Ronald C. McGlennen
Direct Molecular Diagnosis of Multiple Endocrine Neoplasia Type 1
Elizabeth M. Petty, Michael Glynn, and Allen E. Bale
Molecular Detection of Multiple Endocrine Neoplasia Type 2
Edward H. Rowsell and Myra J. Wick
Assay for Detecting the I1307K Susceptibility Allele within the Adenomatous Polyposis Coli Gene
Stephen B. Gruber
Detection of Human Papillomaviruses by Polymerase Chain Reaction and In Situ Hybridization
Elizabeth R. Unger and Suzanne D. Vernon
Molecular Methods for Detecting Epstein-Barr Virus (Part I): Hybridization to Epstein-Barr Virus-Encoded RNA (EBER) Transcripts
Hongxin Fan and Margaret L. Gulley
Molecular Methods for Detecting Epstein-Barr Virus (Part II): Structural Analysis of Epstein-Barr Virus DNA as a Marker of Clonality
Hongxin Fan and Margaret L. Gulley
Molecular Methods for Detecting Epstein-Barr Virus (Part III): EBV Viral Load by Competitive Polymerase Chain Reaction
Hongxin Fan and Margaret L. Gulley
Molecular Detection of Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus-8
Ethel Cesarman
Diagnostic Applications of Quantitative Polymerase Chain Reaction for Cytomegalovirus, Polymerase Chain Reaction System That Detects Herpes Simplex Virus in Cerebrospinal Fluid and Discriminates Genotypes 1 and 2
Yi-Wei Tang
Detection and Typing of Hepatitis C Virus
Frederick S. Nolte
Detection and Speciation of Mycobacteria in Formalin-Fixed, Paraffin-Embedded Tissue Sections
Diana Mohl and Thomas J. Giordano
Ultrasensitive Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma by the AMPLICOR and COBAS AMPLICOR HIV-1 MONITORTM Tests
Steven Herman, James Novotny, and Maurice Rosenstraus
Molecular Diagnosis of Hereditary Thrombotic Disorders
James G. Donnell
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 504
Weight: 731g
Availability: Available
Subcategories: Pathology
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