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MORE ABOUT THIS BOOK
Main description:
Vaccine research and development is advancing at an unprecedented pace, with an increasing emphasis on rational design based upon a fundamental und- standing of the underlying molecular mechanisms. The aim of this volume is to provide a selection of contemporary protocols that will be useful to both novice and advanced practitioner alike. The variety of procedures required to design, develop, produce, and assess a vaccine is immense and covers aspects of ch- istry, biochemistry, molecular biology, cell biology, and immunology. No single volume can hope to cover these topics exclusively. Rather, here we attempt to provide a methods sourcebook focusing on hands-on practical advice. Comp- mentary and background information may be found in other volumes in the Me- ods in Molecular Medicine series. Of particular interest are volumes on Dendritic Cell Protocols, Interleukin Protocols, Vaccine Adjuvants, and DNA Vaccines. Since the publication of the first edition of Vaccine Protocols there have been major advances, particularly in the areas of bacterial genomics, antig- specific T-cell quantification, genetic manipulation of vaccine vectors, the h- nessing of natural molecules concerned with the regulation of immune responses, and the burgeoning field of DNA vaccinology. Hence, the extensive revision of this edition with new chapters on live viral vaccine vectors, atte- ated bacterial vectors, immunomodulators, MHC-peptide tetrameric complexes, and the identification of vaccine candidates by genomic analysis. Additionally, chapters from the first edition have been updated to accommodate state-of-t- art methods in vaccinology.
Contents:
Overview of Vaccines
Gordon Ada
Temperature-Sensitive Mutant Vaccines
Craig R. Pringle
Live Viral Vectors: Construction of a Replication-Deficient Recombinant Adenovirus
Anthony R. Fooks
Live Viral Vectors: Vaccinia Virus
Caroline Staib and Gerd Sutter
Live Viral Vectors: Semliki Forest Virus
Gunilla B. Karlsson and Peter J. Liljestroem
Development of Attenuated Salmonella Strains That Express Heterologous Antigens
Frances Bowe, Derek J. Pickard, Richard J. Anderson, Patricia Londono-Arcila, and Gordon Dougan
Expression and Delivery of Heterologous Antigens Using Lactic Acid Bacteria
Mark A. Reuter, Sean Hanniffy, and Jerry M. Wells
Synthetic Peptides
Michael J. Francis
Genetic Detoxification of Bacterial Toxins
Mariagrazia Pizza, Maria Rita Fontana, Vincenzo Scarlato, and Rino Rappuoli
Preparation of Polysaccharide-Conjugate Vaccines
Carla C. A. M. Peeters, Patrick R. Lagerman, Odo de Weers, Lukas A. Oomen, Peter Hoogerhout, Michel Beurret, Karen M. Reddin, and Jan T. Poolman
Adjuvant Formulations for Experimental Vaccines
Duncan E. S. Stewart-Tull
Incorporation of Immunomodulators into Plasmid DNA Vaccines
Linda S. Klavinskis, Philip Hobson, and Andrew Woods
Microencapsulation of Vaccine Antigens
David H. Jones
Lyophilization of Vaccines: Current Trends
Gerald D. J. Adams
Stimulation of Mucosal Immunity
David J. M. Lewis and Christopher M. M. Hayward
Induction and Detection of T-Cell Responses
Kingston H. G. Mills
Construction of MHC Class I-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune Responses
Rachel V. Samuel and Tomas Hanke
Assessment of Functional Antibody Responses
Ray Borrow and Paul Balmer
The Use of Complete Genome Sequences in VaccineDesign
Nigel J. Saunders and Sarah Butcher
Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment
Michael J. Dennis
Clinical Trials
Paddy Farrington and Elizabeth Miller
Assuring the Quality and Safety of Vaccines: Regulatory Expectations for Licensing and Batch Release
Elwyn Griffiths and Ivana Knezevic
DNA Vaccination: An Update
Douglas B. Lowrie
From Vaccine Research to Manufacture: A Guide for the Researcher
Nigel Allison and Howard S. Tranter
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 428
Weight: 626g
Availability: Available
Subcategories: Immunology
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