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MORE ABOUT THIS BOOK
Main description:
During the past decade, significant progress in molecular and cellular te- niques has greatly advanced our understanding of the wound healing p- cess. Many of these new techniques have been utilized in the context of more classic models of wound healing. The combination of new and classic approaches has allowed scientists to make exciting discoveries in the field of tissue repair, resulting in an explosion of information about the healing p- cess. Importantly, these new findings have great relevance beyond wound healing itself. The injury repair process cuts across many disciplines, exte- ing to such broad fields as cancer, inflammation, and atherosclerosis. The relevance of the field to these many disciplines has generated great interest in models and methods for the study of wound healing. The goal of Wound Healing: Methods and Protocols is to provide scientists from many dis- plines with a compendium of classic and contemporary protocols from r- ognized experts in the field of wound healing. We hope this volume will be useful not only to those working within the field itself, but also to scientists from other disciplines who wish to adapt wound healing models to their own experimental needs. The process of wound healing encompasses many different biologic p- cesses, including epithelial growth and differentiation, fibrous tissue prod- tion and function, angiogenesis, and inflammation.
Contents:
Part I. Experimental Models of Wound Healing
A. In Vivo Animal Models
Excisional Wound Healing: An Experimental Approach
Stefan Frank and Heiko Kampfer
Methods in Reepithelialization: A Porcine Model of Partial-Thickness Wounds
Heather N. Paddock, Gregory S. Schultz, and Bruce A. Mast
Incisional Wound Healing: Model and Analysis of Wound Breaking Strength
Richard L. Gamelli and Li-Ke He
Animal Models of Ischemic Wound Healing: Toward an Approximation of Human Chronic Cutaneous Ulcers in Rabbit and Rat
Mark Sisco and Thomas A. Mustoe
Corneal Injury: A Relatively Pure Model of Stromal-Epithelial Interactions in Wound Healing
Steven E. Wilson, Rahul R. Mohan, Renato Ambrosio, and Rajiv R. Mohan
Subcutaneous Sponge Models
David T. Efron and Adrian Barbul
A Mouse Model of Burn Wounding and Sepsis
Julia M. Stevenson, Richard L. Gamelli, and Ravi Shankar
A Porcine Burn Model
Adam J. Singer and Steve A. McClain
Wound Healing in Airways In Vivo
Steven R. White
Murine Models of Intestinal Anastomoses
David L. Williams and I. William Browder
Murine Model of Peritoneal Adhesion Formation
Andrew E. Jahoda, Mary Kay Olson, and Elizabeth J. Kovacs
Methods for Investigating Fetal Tissue Repair
Ziv M. Peled, Stephen M. Warren, Pierre J. Bouletreau, and Michael T. Longaker
Growth of Human Blood Vessels in Severe Combined Immunodeficient Mice: A New in Vivo Model System of Angiogenesis
Peter J. Polverini, Jacques E. Noer, Martin C. Peters, and David J. Mooney
B. Reviews of Specific Model Systems
Tissue Repair in Models of Diabetes Mellitus: A Review
David G. Greenhalgh
Wound Healing Studies in Transgenic and Knockout Mice: A Review
Richard Grose and Sabine Werner
Wound Repair in Aging: A Review
May J. Reed, TeruhikoKoike, and Pauli Puolakkainen
C. Human Wound Healing Models
Specimen Collection and Analysis: Burn Wounds
Areta Kowal-Vern and Barbara A. Latenser
Suction Blister Model of Wound Healing
Vesa Koivukangas and Aarne Oikarinen
Implantable Wound Healing Models and the Determination of Subcutaneous Collagen Deposition in Expanded Polytetrafluoroethylene Implants
Lars Nannestad Jorgensen, Soren Munk Madsen, and Finn Gottrup
D. In Vitro Models
The Fibroblast Populated Collagen Lattice: A Model of Fibroblast Collagen Interactions in Repair
H. Paul Ehrlich
A Quantifiable In Vitro Model to Assess Effects of PAI-1 Gene Targeting on Epithelial Cell Motility
Kirwin M. Providence, Lisa Staiano-Coico, and Paul J. Higgins
Human Skin Organ Culture
Ingrid Moll
In Vitro Matrigel Angiogenesis Model
Anna M. Szpaderska and Luisa A. DiPietro
Part II. Analysis and Manipulation of Wound Healing
Quantification of Wound Angiogenesis
Quentin E. H. Low and Luisa A. DiPietro
In Vivo Matrigel Migration and Angiogenesis Assays
Katherine M. Malinda
Endothelial Cell Migration Assay: A Quantitative Assay for Prediction of In Vivo Biology
Mark W. Lingen
Analysis of Collagen Synthesis
Robert F. Diegelmann
Method for Detection and Quantitation of Leukocytes During Wound Healing
Iulia Drugea and Aime L. Burns
Detection of Reactive Oxygen Intermediate Production by Macrophages
Jorge E. Albina and Jonathan S. Reichner
Measurement of Chemokines at the Protein Level in Tissue
Robert M. Strieter, Marie D. Burdick, John A. Belperio, and Michael P. Keane
Methods of Measuring Oxygen in Wounds
Harriet W. Hopf, Thomas K. Hunt, Heinz Scheuenstuhl, Judith M. West, Lisa M. Humphrey, and Mark D. Rollins.
Isolation, Culture, and
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 484
Weight: 815g
Availability: Available
Subcategories: General
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