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DNA Vaccines
Methods and Protocols
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Main description:

The field of DNA vaccines has undergone explosive growth in the last few years. As usual, some historical precursors of this approach can be d- cerned in the scientific literature of the last decades. However, the present state of affairs appears to date from observations made discreetly in 1988 by Wolff, Malone, Felgner, and colleagues, which were described in a 1989 patent and published in 1990. Quite surprisingly, they showed that genes carried by pure plasmid DNA and injected in a saline solution, hence the epithet "naked DNA," could be taken up and expressed by skeletal muscle cells with a low but reproducible frequency. Such a simple methodology was sure to spawn many applications. In a separate and important line of experimentation, Tang, De Vit, and Johnston announced in 1992 that it was indeed possible to obtain humoral immune responses against proteins encoded by DNA delivered to the skin by a biolistic device, which has colloquially become known as the "gene gun. " The year 1993 saw the publication of further improvements in the me- ods of naked DNA delivery and, above all, the first demonstrations by several groups of the induction of humoral and cytotoxic immune responses to viral antigens expressed from injected plasmid DNA. In some cases, protection against challenge with the pathogen was obtained. The latter result was - questionably the touchstone of a method of vaccination worthy of the name.


Contents:

Purification of Supercoiled Plasmid, Anthony P. Green. Production of Plasmid DNA in Industrial Quantities According to cGMP Guidelines, Joachim Schorr, Peter Moritz, and Martin Schleef. Development and Characterization of Lyophilized DNA Vaccine Formulations, Nancy L. Shen, Jukka Hartikka, Nancy A. Horn, Marston Manthorpe, and Magda Marquet. Repeated Use of Qiagen Columns in Large-Scale Preparation of Plasmid DNA, Derek Gregory, Ricardo E. Tascon, and Douglas B. Lowrie. The Immunology of DNA Vaccines, Thomas Tuting, Jonathan Austyn, Walter J. Storkus, and Louis D. Falo Jr. Methodology Used in DNA-Based Prophylactic and Therapeutic Immunization Against Hepatitis B Virus in Chimpanzees, Alfred M. Prince and Betsy Brotman. Intramuscular and Intradermal Injection of DNA Vaccines in Mice and Primates, Heather L. Davis. Veterinary DNA Vaccines, Sylvia van Drunen Littel-van den Hurk, Ralph P. Braun, and Lorne A. Babiuk. DNA-Based Immunization of Neonatal Mice, Cynthia L. Brazolot Millan and Heather L. Davis. Intramuscular Injection of DNA Vaccines in Fish, Joel Heppell and Heather L. Davis. Development of DNA Vaccines for Salmonid Fish, Eric D. Anderson and Jo-Ann C. Leong. CTL Analysis for Tumor Vaccines, Antonio Rosato, Gabriella Milan, Annalisa Zambon, Paola Zanovello, and Dino Collavo. The Use of Bone Marrow-Chimeric Mice in Determining the MHC Restriction of Epitope-Specific Cytotoxic T Lymphocytes, Akiko Iwasaki and Brian H. Barber. Immunostimulatory DNA Sequences: An Overview, John H. Van Uden and Eyal Raz. Immunostimulatory CpG Motifs and DNA Vaccines, Risini Weeratna, Arthur M. Krieg, and Heather L. Davis. In Vitro Assay of Immunostimulatory Activities of Plasmid Vectors, Charles F. Reich and David S. Pisetsky. Adjuvants for Plasmid DNA Vaccines, Jon Norman, Jukka Hartikka, Pamela Strauch, and Marston Manthorpe. Cytokine and Costimulatory Factor-Encoding Plasmids as Adjuvants for DNA Vaccination, Kenji Okuda, Susumu Kawamoto, and Jun Fukushima. Optimizationof DNA Vaccines Through the Use of Molecular Adjuvants, Jong J. Kim and David B. Weiner. Cytokine Fusion Constructs as DNA Vaccines Against Tumors, Holden T. Maecker, Athanasia Syrengelas, and Ronald Levy. The Use of Conventional Immunologic Adjuvants in DNA Vaccine Preparations, Shin Sasaki and Kenji Okuda. Genetic Adjuvants, Hildegund C. J. Ertl, Susanna Pasquini, Zhenning He, Hongying Deng, Louise Showe, Wynetta Giles-Davis, Yijie Wang, InSug O, Hilary Marston, Magdalena Blaszczyk- Thurin, and Zhiquan Xiang. DNA Immunization in Combination with the Immunostimulant Monophosphoryl Lipid A, Donald L. Lodmell, Nancy B. Ray, and Larry C. Ewalt. Controlled Plasmid Delivery and Gene Expression: Applications for Nucleic Acid-Based Vaccines, Russell J. Mumper, Harry C. Ledebur, Jr., Alain P. Rolland, and Eric Tomlinson. Mucosal Immunization with DNA Vaccines, Michael J. McCluskie and Heather L. Davis. Preparations for Particle-Mediated Gene Transfer Using the Accell (R) Gene Gun, Michael D. Macklin, Robert J. Drape, and William F. Swain. Entrapment of Plasmid DNA Vaccines into Liposomes by Dehydration/Rehydration, Gregory Gregoriadis, Brenda McCormack, Mia Obrenovich, and Yvonne Perrie. DNA-Based Vaccination with Polycistronic Expression Plasmids, Reinhold Schirmbeck, Jan von Kampen, Karin Metzger, Jens Wild, Beate Gruner, Martin Schleef, Andrea Kroeger, Hansjoerg Hauser, and Joerg Reimann. A Nonviral Cytoplasmic T7 Autogene System and Its Applications in DNA Vaccination, Franck G. Sturtz, Yunsheng Li, Janine Shulok, H. Ralph Snodgrass, and Xiao-zhuo Chen. Immunization with Naked DNA Coexpressing Antigen and Cytokine via IRES, Jochen Heinrich, Bettina Strack, Michael Nawrath, Jovan Pavlovic, and Karin Moelling. Genetic Subunit Vaccines: A Novel Approach for Genetic Immunization, I. Frank Ciernik and David P. Carbone. Antigen Engineering in DNA Immunization, Shan Lu, Steve Manning, and James Arthos. Genetic Vaccination Targeting T-Cell Receptors, Alexis P. Godillot, Qiong


PRODUCT DETAILS

ISBN-13: 9781489941824
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2013
Pages: 529
Weight: 836g
Availability: Available
Subcategories: Pharmacology
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