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MORE ABOUT THIS BOOK
Main description:
In Volume I, Analysis of Cells and Tissues, we presented a range of protocols aimed at mapping and analyzing the expression of various molecules of pot- tial interest in metastasis research and for examining their production at the genetic level. In this second volume of metastasis research protocols, we move to the level of living cells and tissues and present methodologies applicable to examining metastatic behavior in vitro and in whole animal models. The methods described in the first section of this volume concentrate on the separation of cell lines with high and low metastatic potential, including the genetic modification of cell lines. The assay systems to test defined aspects of the metastatic cascade are then described in Part II and include cell migration assays, assays for matrix degrading enzymes, basement membrane degrading assays, adhesion assays, and assays of angiogenesis. The role of the specific elements of the metastatic cascade assayed in each of these systems in turn must of course be put into perspective relative to their roles in entire living organisms.
Contents:
Part I. Selection/Segregation of Cell Populations for In vitro and In Vivo Assays of Metastatic Behavior
Cell Separations by Flow Cytometry
Derek Davies
Immunomagnetic Cell Separation
Catherine Clarke and Susan Davies
Genetic Modification of Cell Lines to Enhance Their Metastatic Capability
Daniel McWilliams and Hilary Collins
Part II. In Vitro Assays of Metastatic Behavior
Cell Aggregation Assays, Tom Boterberg
Marc E. Bracke, Erik A. Bruyneel, and Marc M. Mareel
Cell Migration and the Boyden Chamber
Nicholas S. Brown and Roy Bicknell
Quantification of Cell Motility: Gold Colloidal Phagokinetic Track Assay and Wound Healing Assay
Yasufumi Niinaka, Arayo Haga, and Avraham Raz
In Vitro Invasion Assay Using Matrigel (R)
Debbie M. S. Hall and Susan A. Brooks
Membrane Invastion Culture System
Mary J. C. Hendrix, Elisabeth A. Seftor, and Richard E. B. Seftor
Collagen Invasion Assay
Marc E. Bracke, Tom Boterberg, Erik A. Bruyneel, and Marc M. Mareel
Chick Heart Invasion Assay
Marc E. Bracke, Tom Boterberg, and Marc M. Mareel
Adhesion of Tumor Cells to Matrices and Endothelium
G. Ed Rainger
Assessment of Angiogenic Factors: The Chick Chorioallantoic Membrane Assay
Adam Jones, Chisato Fujiyama, Stephen Hague, and Roy Bicknell
Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth and Metastasis
Nerbil Kilic and Suleyman Ergun
Galectin-3 Binding and Metastasis
Pratima Nangia-Makker, Yuichiro Honjo, and Avraham Raz
Part III. Animal Models of Metastasis
Basic Principles for the Study of Metastasis Using Animal Models
Suzanne A. Eccles
The Chick Embryo Metastasis Model
Eric Petitclerc and Peter C. Brooks
Gene Transfection for Metastasis ResearchUsing Animal Models
Ruth J. Muschel and Jim Hua
Theoretical Considerations in Using Animal Models of Metastastis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice
Sue A. Watson and Teresa M. Morris
Xenograft Models in Immunodeficient Animals: I. Nude Mice: Spontaneous and Experimental Metastatis Models
Janet E. Price
Xenograft Models in Immunodeficient Animals: II. Use of SCID Mice in Metastasis Research: Breast and Colon Cancer Models of Metastasis
Udo Schumacher and Susan A. Brooks
Syngeneic Murine Metastasis Model: B16 Melanoma
Raffaella Giavazzi and Angela Garofalo
Transgenic Animal Models
Chantale Guy and Gizela Cordosa
Clinically Accurate Orthotopic Mouse Models of Cancer
Robert M. Hoffman
Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex Vivo
Marian Rocha, Volker Schirrmacher, and Victor Umansky
Green Fluorescent Protein for Metastastis Research
Robert M. Hoffman
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 332
Weight: 489g
Availability: Available
Subcategories: Oncology
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