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Main description:
The Plasmodium spp. parasite was identified as the causative agent of malaria in 1880, and the mosquito was identified as the vector in 1897. Despite subsequent efforts focused on the epidemiology, cell biology, immunology, molecular biology, and clinical manifestations of malaria and the Plasmodium parasite, there is still no licensed vaccine for the prevention of malaria. Physical barriers (bed nets, window screens) and chemical prevention methods (insecticides and mosquito repellents) intended to interfere with the transmission of the disease are not highly effective, and the profile of resistance of the parasite to chemoprophylactic and chemotherapeutic agents is increasing. The dawn of the new millennium has seen a resurgence of interest in the disease by government and philanthropic organizations, but we are still faced with compl- ities of the parasite, the host, and the vector, and the interactions among them. Malaria Methods and Protocols offers a comprehensive collection of protocols describing conventional and state-of-the-art techniques for the study of malaria, as well as associated theory and potential problems, written by experts in the field. The major themes reflected here include assessing the risk of infection and severity of disease, laboratory models, diagnosis and typing, molecular biology techniques, immunological techniques, cell biology techniques, and field applications.
Contents:
Part I. Assessing Risk of Infection and Severity of Disease
Vector Incrimination and Entomological Inoculation Rates
John C. Beier
Epidemiological Measures of Risk of Malaria
J. Kevin Baird, Michael J. Bangs, Jason D. Maguire, and Mazie J. Barcus
Part II. Laboratory Models
Maintenance of the Plasmodium berghei Life Cycle
Robert E. Sinden, Geoff A. Butcher, and A. L. Beetsma
Mouse Models for Pre-Erythrocytic-Stage Malaria
Laurent Renia, Elodie Belnoue, and Irene Landau
Mouse Models for Erythrocytic-Stage Malaria
Latifu A. Sanni, Luis F. Fonseca, and Jean Langhorne
Nonhuman Primate Models : I. Nonhuman Primate Host-Parasite Combinations
William E. Collins
Nonhuman Primate Models : II. Infection of Saimiri and Aotus Monkeys with Plasmodium vivax
William E. Collins
Part III. Diagnosis and Typing
Vector Analysis
John C. Beier
Genotyping of Plasmodium spp.: Nested PCR
Georges Snounou
Genotyping of Plasmodium falciparum: PCR-RFLP Analysis
Ingrid Felger and Hans-Peter Beck
Microsatellite Analysis in Plasmodium falciparum
Xin-zhuan Su and Michael T. Ferdig
Quantitation of Liver-Stage Parasites by Automated TaqMan (R) Real-Time PCR
Adam A. Witney, Robert M. Anthony, and Daniel J. Carucci
Quantitation of Liver-Stage Parasites by Competitive RT-PCR
Kyle C. McKenna and Marcelo R. S. Briones
Part IV. Molecular Biology Techniques
Extraction and Purification of Plasmodium Total RNA
Till Voss
Extraction and Purification of Plasmodium Parasite DNA
Hans-Peter Beck
Southern Blotting of Parasite DNA
Tobias Spielmann
SDS-PAGE and Western Blotting of Plasmodium falciparum Proteins
Roland A. Cooper
Nested PCR Analysis of Plasmodium Parasites
Georges Snounou and BalbirSingh
RFLP Analysis
Hans-Peter Beck
Analysis of Gene Expression by RT-PCR
Peter Preiser
In Situ Detection of RNA in Blood- and Mosquito-Stage Malaria Parasites
Joanne Thompson
Purification of Chromosomes from Plasmodium falciparum
Daniel J. Carucci, Paul Horrocks, and Malcolm J. Gardner
Construction of Genomic Libraries from the DNA of Plasmodium Species
Leda M. Cummings, Dharmendar Rathore, and Thomas F. McCutchan
Construction of a Gene Library with Mung Bean Nuclease-Treated Genomic DNA
Dharmendar Rathore and Thomas F. McCutchan
Construction of Plasmodium falciparum l cDNA Libraries
David A. Fidock, Dharmendar Rathore, and Thomas F. McCutchan
Production of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive Hybridization
David A. Fidock, Thanh V. Nguyen, Brenda T. Beerntsen, and Anthony A. James
Construction and Screening of YAC Libraries
Cecilia P. Sanchez, Martin Preuss, and Michael Lanzer
Episomal Transformation of Plasmodium berghei
Chris J. Janse and Andrew P. Waters
Gene Targeting in Plasmodium berghei
Vandana Thathy and Robert Menard
Part V. Immunological Techniques
Peptide Vaccination, Valentin Meraldi
Jackeline F. Romero, and Giampietro Corradin
DNA Vaccination
Richard C. Hedstrom and Denise L. Doolan
Assessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant Viruses
Moriya Tsuji
Assessing CD4+ Helper T-Lymphocyte Responses by Lymphoproliferation
Isabella A. Quakyi and Jeffrey D. Ahlers
Limiting Dilution Analysis of Antigen-Specific CD4+ T-Cell Responses in Mice
Elsa Seixas, Jean Langhorne, and Stuart Quin
Cell Trafficking: Malaria Blood-Stage Parasite-Specific CD4+ T Cells After Adoptive Transfer into Mice
Chakrit
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 648
Weight: 1215g
Availability: Available
Subcategories: Infectious Diseases
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