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Main description:
A qualitative leap in the understanding of cardiovascular and n- ral regulation by the renin-angiotensin system, and of the role of this s- tem in tissue damage, has occurred as a result of the many recent advances in molecular genetic techniques. The cloning of the genes for the components of the renin-angiotensin system, the design of specific angiotensin receptor ligands, and the use of embryonic gene targeting te- niques for the creation of mutant strains have established that the renin- angiotensin system is important in blood pressure regulation, ion and fluid homeostasis, and tissue growth and remodeling Further investigation of the mechanisms by which this system p- ticipates in cardiovascular regulation may shed some light on the pat- genesis of several cardiovascular diseases, e. g. , hypertension, congestive heart failure, and chronic renal failure. Despite the promise of this system as a target for therapeutic interventions for these diseases, there are great challenges in the integration of the attempts to close the gap between the traditional literature of medicine and the explosion of information from the new technologies. This book's title, Angiotensin Protocols, reflects the authors' strong efforts to translate expert knowledge into easy-to-follow practice. The book opens with introductory chapters, and each specialty section provides detailed methods covering a wide variety of techniques, ranging from genetic manipulation of targeted genes to functional studies of the renin- angiotensin system.
Contents:
Part I. Introduction to the Renin-Angiotensin System
Historical Perspective of the Renin-Angiotensin System
John E. Hall
Three Angiotensin Paradigms in One Patient: Etiology of Hypertension, Glomerular Hemodynamics, and Long-Term Glomerular Protection
Cheryl L. Laffer and Fernando Elijovich
Part II. Transgenic and Gene Targeting Methods
Production and Use of Chimeric Mice
Junji Takaya, Taiji Matsusaka, and Iekuni Ichikawa
Genetic Manipulation of the Renin-Angiotensin System Using Cre-loxP-Recombinase
Curt D. Sigmund and David E. Stec
Systemic Delivery of a Transgene in Intact Animals by a Retroviral Vector
Michael J. Katovich, Hong-Wei Wang, Craig H. Gelband, and Mohan K. Raizada
Antisense Inhibition of the Renin-Angiotensin System
Dagmara Mohuczy and M. Ian Phillips
Part III. Analysis of Transcriptional and Translational Mechanisms
Analysis of Transcriptional Control Mechanisms I: Techniques for Characterization of cis-Regulatory Elements
Christopher T. Sherman and Allan R. Brasier
Analysis of Transcriptional Control Mechanisms II: Techniques for Characterization of trans-Acting Factors
Allan R. Brasier, Christopher T. Sherman, and Mohammad Jamaluddin
Analysis of Cytosolic Proteins that Bind to the 5' Leader Sequence of the Angiotensin AT1 Receptor by RNA Electromobility Shift Assay
Zheng Wu, Kamakshi Krishnamurthi, Koby Mok, and Kathryn Sandberg
In Vitro Translation of the Angiotensin AT1 Receptors in Wheat-Germ Extracts
Hong Ji, Kamakshi Krishnamurthi, Zheng Wu, and Kathryn Sandberg
Part IV. Assays for Determination of mRNA Levels
Quantitation of Angiotensin II Receptors by Competitive Reverse-Transcription Polymerase Chain Reaction
Wei Wu, Aqing Yao, and Donna H. Wang
Analysis of RNA by Northern-Blot Hybridization
Patricia E. Gallagher and Debra I. Diz
Use of the Ribonuclease Protection Assay for the Analysis and Characterization of Target mRNAs
Scott C. Supowit and Donald J. DiPette
In Situ Hybridization
Susan Riggs Runge, Zhaoyong Hu, and Marschall Runge
Part V. Assays for Determination of Protein Contents and Enzyme Activity
Immunohistochemical Detection of Angiotensin II Receptor
Zhi-Qin Wang and Robert M. Carey
Western Blotting
Nageswara R. Madamanchi and Marschall S. Runge
Quantification of Angiotensin-Converting Enzyme (ACE) Activity
Qing Cheng Meng and Kathleen H. Berecek
Quantification of Angiotensin Peptides
Qing Cheng Meng and Kathleen H. Berecek
Radiolabeling of Angiotensin Peptides
Robert C. Speth and Joseph W. Harding
Assays for Radiolabel-Photoaffinity Labeling of Angiotensin Receptors
Jacqueline Perodin, Antony A. Boucard, Richard Leduc, Emanuel Escher, and Gaetan Guillemette
Radioligand Binding Assay
Gaetan Thibault and Ernesto L. Schiffrin
Autoradiographic Localization and Quantification of Components of the Renin-Angiotensin System in Tissues
Siew Yeen Chai, Andrew M. Allen, Jialong Zhuo, Ingrid Moeller, and Frederick A. O. Mendelsohn
Part VI. Biochemical Studies of the Renin-Angiotensin System
Measurement of Intracellular Free Calcium Ion Concentration in Vascular Smooth Muscle Cells: Fluorescence Imaging of Cytosolic Calcium
Rhian M. Touyz and Ernesto L. Schiffrin
Measurement of Phospholipidase D Activation in Vascular Smooth Muscle Cells
Rhian M. Touyz and Ernesto L. Schiffrin
Measurement of Cyclic AMP and Cyclic GMP in Xenopus Oocytes Stimulated with Angiotensin II and Atrial Natriuretic Factor
Hong Ji
Measurement of Nitric Oxide Formation
Christian Thorup and Leon C. Moore
Measuring Renin Secretion from Juxtaglomerular
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2011
Pages: 544
Weight: 788g
Availability: Available
Subcategories: Cardiovascular Medicine, Physiology
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