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MORE ABOUT THIS BOOK
Main description:
A comprehensive collection of classic and innovative methodologies used in many laboratories for the investigation of multiple myeloma. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicine (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. These proven techniques are ideal for studying the pathogenesis of multiple myeloma and identifying new therapeutic targets.
Contents:
Multiple Myeloma: Challenges and Opportunities
Douglas Joshua, Ross D. Brown, and P. Joy Ho
Immunophenotyping of Plasma Cells in Multiple Myeloma
Gema Mateo Manzanera, Jesus F. San Miguel Izquierdo, and Alberto Orfao de Matos
Plasma Cell Labeling Index
Philip R. Greipp and Shaji Kumar
Conventional Cytogenetics in Myeloma
Lynda J. Campbell
Multicolor Spectral Karyotyping in Multiple Myeloma
Jeffrey R. Sawyer
Detection of Chromosome 13 Deletions by Fluorescent In Situ Hybridization
Scott VanWier and Rafael Fonseca
Comparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple Myeloma
Pulivarthi H. Rao
Southern Blotting of IgH Rearrangements in B-Cell Disorders
Edna Yuen and Ross D. Brown
Identification and Assembly of V Genes As Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloma
Surinder S. Sahota, Mark Townsend, and Freda K. Stevenson
Identification of Clonotypic IgH VDJ Sequences in Multiple Myeloma
Brian J. Taylor, Jitra Kriangkum, Erin R. Strachan, Juanita Wizniak, and Linda M. Pilarski
Real-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of Minimal Residual Disease in Myeloma
Mara Compagno, Barbara Mantoan, Monica Astolfi, Mario Boccadoro, and Marco Ladetto
Incomplete DJH Rearrangements
David Gonzalez and Ramon Garcia-Sanz
Identification of Malignant Plasma Cells by mRNA In Situ Hybridization
Ross D. Brown and P. Joy Ho
The SCID-hu Myeloma Model
Joshua Epstein and Shmuel Yaccoby
The 5T2MM Murine Model of Multiple Myeloma: Maintenance and Analysis
Karin Vanderkerken, Kewal Asosingh, Angelo Willems, Hendrik De Raeve, Pedro Couck, Frans Gorus, Peter Croucher, and Ben Van Camp
Determination of Telomerase Activity and Telomere Length
Kai-da Wu andMalcolm A. S. Moore
Dendritic Cell/Myeloma Hybrid Vaccine
Dajing Xia, Tim Chan, and Jim Xiang
Genetically Engineered Myeloma Cell Vaccine
Siguo Hao, Tim Chan, and Jim Xiang
An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factors
Andrew C. W. Zannettino, Amanda N. Farrugia, L. Bik To, and Gerald J. Atkins
Clonality Detection of the Expanded T-Cell Populations in Patients With Multiple Myeloma
Daniel M-Y. Sze
Detecting Mismatch Repair Defects in Myeloma
Mark Velangi, Elizabeth Matheson, Andrew Hall, and Julie Irving
Methylation-Specific Polymerase Chain Reaction
Oliver Galm and James G. Herman
Making Sense of DNA Microarray Data
Wei-min Liu, Sunhee K. Ro, and Walter H. Koch
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 324
Weight: 546g
Availability: Available
Subcategories: Haematology
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