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MORE ABOUT THIS BOOK
Main description:
This latest addition to the Methods in Molecular Medicine series, Anti- ral Methods and Protocols, is opportune because there is an increasing int- est in discovering compounds that are effective against both chronic and acute viral infections. A number of the methods described in the volume are unp- lished and their inclusion indicates the speed at which this field is moving. This volume is not a review but each chapter contains methods validated by the experts who have spent time in developing the protocols. The hallmark of this series is the comprehensive way in which the me- ods are described, which includes a list of all the reagents needed for each protocol. Of importance is the section on tips and pitfalls that the authors have discovered while developing their protocols. The manual itself is designed to be used by researchers in universities and industry who are familiar with a range of biological techniques but who want to set up quickly a novel assay system. We encourage a dialog between readers and authors, which may also result in useful collaborations.
Contents:
Changing Methods for Discovering Antiviral Drugs
Philip S. Jones
Laboratory Safety Considerations
Paul M. Feorino, John D. Williamson, and Raymond F. Schinazi
Routine Quality Control Testing of Cell Cultures: Detection of Mycoplasma
Alison Stacey and Glyn Stacey
Part I. Hepatitis Virus
Hep AD38 Assay: A High-Throughput, Cell-Based Screen for the Evaluation of Compounds Against Hepatitis B Virus
Robert W. King and Stephanie K. Ladner
Hepatitis B Virus Cell Culture Assays for Antiviral Activity
Karen Schmidt and Brent Korba
A Rapid Microtiter Assay for Duck Hepatitis Virus Reverse Transcriptase
Michael J. Otto and Richard A. Whitaker
Assays for Measuring Hepadnaviral Supercoiled DNA
Stephen A. Locarnini and Gilda M. Civitico
Assays for Hepatitis B Virus DNA- and RNA-Dependent DNA Polymerase Activities
Timothy Shaw and Stephen A. Locarnini
Development of a Hepatitis C Virus RNA Helicase High Throughput Assay
Ann D. Kwong and Christine Risano
Part II. Herpesvirus
Determination of Viral Infectivity: HSV, VZV, and CMV
David R. Harper
Antiviral Screening Assays for Human Herpesviruses 6 and 7
Jodi B. Black and Philip E. Pellett
Strategies for Evaluation of Antiviral Agents Against Epstein-Barr Virus in Culture
Jung-Chung Lin
Monitoring Drug Resistance for Herpesviruses
Graciela Andrei, Pierre Fiten, Erik De Clercq, Robert Snoeck, and Ghislain Opdenakker
Herpesvirus Protease Assays
Peter Ertl, Linda Russell, and Jane Angier
Part III. Human Immunodeficiency Virus
Testing Compounds for Antiviral Activity in Cell Cultures Infected with HIV
Donna Devine, Naomi Mathews, and Derek Kinchington
Evaluation of Compounds That Prevent Reactivation of HIV-1 in OM-10.1 Cells
Salvatore T. Butera
Assessment of Activity of Topical Virucidal Agents
Timothy O'Connor
Assays That Measure Selective Killing of Virus-Infected Cells
Sharon Randall
Evaluating Clinical Isolates for Their Phenotypic and Genotypic Resistance Against Anti-HIV Drugs
Anne-Mieke Vandamme, Myriam Witvrouw, Christophe Pannecouque, Jan Balzarini, Kristel Van Laethem, Jean-Claude Schmit, Jan Desmyter, and Erik De Clercq
Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase
Lieven Stuyver, Ann Wyseur, Gonda Verpooten, and Rudi Rossau
RT-PCR for Amplification of Specific Fragments of HIV-1 Genome
Marie-Pierre de Bethune and Kurt Hertogs
Evaluation of Compounds Against Recombinant HIV Reverse Transcriptase
Jan Balzarini
Ultrasensitive Detection of Reverse Transcriptase Activity by the Amp-RT Assay: Applications to the Measurement of Virus Loads and Phenotypic Drug Resistance
J. Gerardo Garcia Lerma and Walid Heneine
Reverse Transcriptase Assay Based on Product Enhancement for Assessing the Drug Susceptibility of Retroviruses
Jurg Boeni and Joerg Schupbach
Identifying and Characterizing HIV Protease Inhibitors
Eric S. Furfine
Retroviral Integrase: A Novel Target in Antiviral Drug Development and Basic In Vitro Assays with the Purified Enzyme
Abhijit Mazumder, Nouri Neamati, Sanjay Sunder, Joshua Owen, and Yves Pommier
Part IV. Human Papilloma Virus
Papillomavirus DNA Replication
Maureen C. Ferran and Alison A. McBride
Quantitative Immunodetection of Viral Oncoprotein Levels in Human Papillomavirus-Transformed Human Cells
Jeffery A. Johnson and J. David Gangemi
Part V. Influenza Viruses
Biochemical Methods for the Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en
Richard C. Bethell, Graham J. Hart, Tony J. Blick, Anjali Sahasrabudhe,
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2011
Pages: 420
Weight: 640g
Availability: Available
Subcategories: Infectious Diseases, Pharmacology
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