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Main description:
Blood-brain barrier (BBB) breakdown leading to cerebral edema occurs in many brain diseases-such as trauma, stroke, inflammation, infection, and tumors-and is an important factor in the mortality arising from these con- tions. Despite the importance of the BBB in the pathogenesis of these diseases, the molecular mechanisms occurring at the BBB are not completely und- stood. In the last decade a number of molecules have been identified not only in endothelial cells, but also in astrocytes, pericytes, and the perivascular cells that interact with endothelium to maintain cerebral homeostasis. However, the precise cellular interactions at a molecular level in steady states and d- eases have still to be determined. The introduction of new research techniques during the last decade or so provide an opportunity to study the molecular mec- nisms occurring at the BBB in diseases. The Blood-Brain Barrier: Biology and Research Protocols provides the reader with details of selected morphologic, permeability, transport, in vitro, and molecular techniques for BBB studies, all written by experts in the field. Each part is preceded by a review that emphasizes the advantages and pitfalls of particular techniques, as well as offering much relevant current information. The techniques provided will be helpful to both beginners in BBB research and those more experienced investigators who wish to add a specific technique to those already available in their laboratories.
Contents:
Part I. Tissue Techniques
Morphology and Molecular Properties of Cellular Components of Normal Cerebral Vessels
Sukriti Nag
Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry
Sukriti Nag
Freeze-Fracture Studies of Cerebral Endothelial Tight Junctions
Hartwig Wolburg, Stefan Liebner, and Andrea Lippoldt
Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy
Albert S. Lossinsky and Richard R. Shivers
Detection of Endothelial/Lymphocyte Interaction in Spinal Cord Microvasculature by Intravital Videomicroscopy
Britta Engelhardt, Peter Vajkoczy, and Melanie Laschinger
Part II. Blood-Brain Barrier Permeability Techniques
Pathophysiology of Blood-Brain Barrier Breakdown
Sukriti Nag
Pial Vessel Permeability to Tracers Using Cranial Windows
William G. Mayhan
Blood-Brain Barrier Permeability Using Tracers and Immunohistochemistry
Sukriti Nag
Ultracytochemical Studies of the Compromised Blood-Brain Barrier
Sukriti Nag
Immunogold Detection of Microvascular Proteins in the Compromised Blood-Brain Barrier
Eain M. Cornford, Shigeyo Hyman, and Marcia E. Cornford
Measurement of Blood-Brain and Blood-Tumor Barrier Permeabilities with [14C]-Labeled Tracers
Kamlesh Asotra, Nagendra Ningaraj, and Keith L. Black
Part III. Blood-Brain Barrier Transport Techniques
A Review of Blood-Brain Barrier Transport Techniques
Quentin R. Smith
In Situ Brain Perfusion Technique
Quentin R. Smith and David D. Allen
Brain Efflux Index Method: Characterization of Efflux Transport Across the Blood-Brain Barrier
Hiroyuki Kusuhara, Tetsuya Terasaki, and Yuichi Sugiyama
Transport Studies Using Membrane Vesicles
Darryl R. Peterson and Richard A. Hawkins
Drug Transport Studies Using Quantitative Microdialysis
Haiqing Dai and William F. Elmquist
Single-Pass Dual-Label Indicator Method: Blood-to-Brain Transport of Glucose and Short-Chain Monocarboxylic Acids
Michelle A. Puchowicz, Kui Xu, and Joseph C. LaManna
Protein Transport in Cerebral Endothelium: In Vitro Transcytosis of Transferrin
Laurence Fenart and Romeo Cecchelli
Transport Across the Choroid Plexuses In Vivo and In Vitro
Nathalie Strazielle and Jane Elizabeth Preston
Part IV. In Vitro Techniques
An Overview of In Vitro Techniques for Blood-Brain Barrier Studies
Andreas Reichel, David J. Begley, and N. Joan Abbott
Isolation and Characterization of Human Brain Endothelial Cells
Katerina Dorovini-Zis, Rukmini Premeya, and Hanh Huynh
Human Brain Microvessel Endothelial Cell and Leukocyte Interactions
Jacqueline Shukaliak-Quandt, Donald Wong, and Katerina Dorovini-Zis
Development and Characterization of Immortalized Cerebral Endothelial Cell Lines
Pierre-Olivier Couraud, John Greenwood, Francoise Roux, and Pete Adamson
Isolation and Characterization of Retinal Endothelial Cells
David A. Antonetti and Ellen B. Wolpert
Isolation and Characterization of Cerebral Microvascular Pericytes
Paula Dore-Duffy
Part V. Molecular Techniques
Molecular Biology of the Blood-Brain Barrier
William M. Pardridge
Blood-Brain Barrier Genomics
Ruben J. Boado
Dynamic In Vitro Model of the Blood-Brain Barrier: Gene Profiling Using cDNA Microarray Analysis
Matteo Marroni, Kelly M. Kight, Mohammed Hossain, Luca Cucullo, Shailesh Y. Desai, and Damir Janigro
Phage Display Technology for Identifying Specific Antigens on Brain Endothelial Cells
Jamshid Tanha, Arumugam Muruganandam, and Danica Stanimirovic
mRNA Detection in Cerebral Vessels by Nonradioactive In Situ
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 564
Weight: 1175g
Availability: Available
Subcategories: Neuroscience
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