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Main description:
An indispensable collection of updated classical and emerging techniques that promise to add critical knowledge to our understanding of cartilage metabolism in health and disease. Volume 1: Cellular and Molecular Tools describes proven molecular and cellular techniques for the in vitro study of normal and osteoarthritic cartilage through biochemical, biomolecular, immunological, and physical approaches, with emphasis on the genetic manipulation of cells. Volume 2: Structure and In Vivo Analysis, offers cutting-edge procedures for the study-at the tissue level-of turnover, structure, and functioning of normal and diseased articular cartilage by invasive and noninvasive means. Comprehensive and up-to-date, the two volumes of Cartilage and Osteoarthritis provide researchers and bench scientists with readily reproducible protocols for new experiments to understand-from the cellular to the animal level-the pathophysiology of cartilage and to discover molecular targets for pharmacological intervention.
Contents:
Generation and Use of Transgenic Mice As Models of Osteoarthritis
Anna-Marja Saamanen and Eero Vuorio
Development and Clinical Application in Arthritis of a New Immunoassay for Serum Type IIA Procollagen NH2 Propeptide
Jean-Charles Rousseau, Linda J. Sandell, Pierre D. Delmas, and Patrick Garnero
Histological and Immunohistological Studies on Cartilage
James Melrose, Susan Smith, and Peter Ghosh
Histochemical Visualization of the Cartilage Hyaladherins Using a Biotinylated Hyaluronan Oligosaccharide Bioaffinity Probe
James Melrose and Susan Smith
Methods for Cartilage and Subchondral Bone Histomorphometry
Philippe Pastoureau and Agnes Chomel
Laser-Mediated Microdissection As a Tool for Molecular Analysis in Arthritis
Martin Judex, Elena Neumann, Steffen Gay, and Ulf Muller-Ladner
Analysis of Protein Distribution in Cartilage Using Immunofluorescence and Laser Confocal Scanning Microscopy
Stephan Soeder, Alexander Kuhlmann, and Thomas Aigner
Molecular and Biochemical Assays of Cartilage Components
Caroline D. Hoemann
Mechanical Characterization of Native and Tissue-Engineered Cartilage
Albert C. Chen, Stephen M. Klisch, Won C. Bae, Michele M. Temple, Kevin B. McGowan, Kenneth R. Gratz, Barbara L. Schumacher, and Robert L. Sah
Noninvasive Study of Human Cartilage Structure by MRI
Felix Eckstein
High-Resolution MRI Techniques for Studies in Small-Animal Models
Olivier Beuf
High-Resolution Imaging of Osteoarthritis Using Microcomputed Tomography
Lydia Wachsmuth and Klaus Engelke
High-Resolution Ultrasonography for Analysis of Age- and Disease-Related Cartilage Changes
Amena Saied and Pascal Laugier
Evaluation of Cartilage Composition and Degradation by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance
Jurgen Schiller, Daniel Huster, BeateFuchs, Lama Naji, Joern Kaufmann, and Klaus Arnold
Pulsed-Field Gradient-Nuclear Magnetic Resonance (PFG NMR) to Measure the Diffusion of Ions and Polymers in Cartilage: Applications in Joint Diseases
Jurgen Schiller, Lama Naji, Robert Trampel, Wilfred Ngwa, Robert Knauss, and Klaus Arnold
Dynamics of Collagen in Articular Cartilage Studied by Solid-State NMR Methods
Daniel Huster, Jurgen Schiller, and Klaus Arnold
Computerized Protocol for Anatomical and Functional Studies of Joints
Sandra Martelli and Stefano Zaffagnini
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 358
Weight: 527g
Availability: Available
Subcategories: Orthopaedics and Fractures
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