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Main description:
This is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular. These volumes details novel technologies, some of which are still evolving and whose impacts are yet to be determined. Leaders in the field contribute to cover exciting and cutting edge topics. This compendium will be a valuable tool.
Contents:
Arthritis Research: Methods and Protocols, Volume I
Andrew P. Cope, PhD
I. Synovial Joint Morphology, Histopathology, and Immunohistochemistry
1. Imaging Inflamed Synovial Joints
Ai Lyn Tan, Helen I. Keen, Paul Emery, and Dennis McGonagle
2. Arthroscopy as a Research Tool: A Review
Richard J. Reece
3. Immunohitochemistry of the Inflamed Synovium
Martina Gogarty and Oliver FitzGerald
4. In Situ Hybridisation of Synovial Tissue
Stefan Kuchen, Christian A. See,ayer, Michel Neidhart, Renate E. Gay, and Steffen Gay
5. Subtractive Hybridization
Joerg H. W. Distler, Oliver Distler, Michel Neidhart, and Steffen Gay
6. Laser Capture as a Tool for Analysis of Gene Expression in Inflamed Synovium
Ulf Muller-Ladner, Martin Judex, Elena Neumann and Steffen Gay
7. Preparation of Mononuclear Cells from Synovial Tissue
Jonathan T. Beech and Fionula M. Brennan
8. Quantitative Image Analysis of Synovial Tissue
Pascal O. van der Hall, Maarten C. Kraan and Paul Peter Tak
II. Cartilage Matrix and Bone Biology
9. Cartilage Histomorphometry
Ernst B. Hunziker
10. Image Analysis of Aggrecan Degradation in Articular Cartilage with Formalin-Fixed Samples
Barbara Osborn, Yun Bai, Anna H.K. Plaas, and John D. Sandy
11. In Situ Detection of Cell Death in Articular Cartilage
Samantha N. Redman, Ilyas M. Khan, Simon R. Tew and Charles W. Archer
12. Measurement of Glycosaminoglycan Release from Cartilage Explants
John S. Mort and Peter J. Roughley
13. Assessment of Collagenase Activity in Cartilage
Tim E.Cawston and Tanya G. Morgan
14. Assessment of Gelatinase Expression and Activity in Articular Cartilage
Rosalind M. Hembry, Susan J. Atkinson and Gillian Murphy
15. Analysis of MT1-MMP Activity
Richard D. Evans and YoshifumiItoh
16. Analysis of TIMP Expression and Activity
Linda Troeberg and Hideaki Nagase
17. Bone Histomorphology in Arthritis Models
Georg Schett and Birgit Tuerk
18. Generation of Osteoclasts in vitro and Assay of Osteoclast Activity
Naoyuki Takahashi, Nobuyuki Udagawa, Yasuhiro Kobayashi and Tatsuo Suda
III. Cell Trafficking, Migration and Invasion
19. Isolation and Analysis of Large and Small Vessel Endothelial Cells
Justin C. Mason, Elaine A Lidington and Helen Yarwood
20. Analysis of Flow Based Adhesion in vitro
Oliver Florey and Dorian O. Haskard
21. Analysis of Leukocyte Recruitment in Synovial Microcirculation by Intravital Microscopy
Gabriela Constantin
22. Angiogenesis in Arthritis - Methodological and Analytical Details
Ursula Fearon and Douglas J. Veale
23. Analysis of Inflammatory Leukocyte and Endothelial Chemotactic Activity
Zoltan Szekanecz and Alisa Koch
24. Acquisition, Culture and Phenotyping of Synovial Fibroblasts
Sanna Rosengren, David L. Boyle and Gary S. Firestein
25. Genotyping of Synovial Fibroblasts: cDNA Array in Combination with RAP-PCR in Arthritis
Elena Neumann, Martin Judex, Steffen Gay and Ulf Muller-Ladner
26. Gene Transfer to Synovial Fibroblast - Methods and Evaluation in the SCID Mouse Model
Ingmar Meinecke, Edita Rutkauskaite, Antje Cinski, Ulf Muller-Ladner, Steffen Gay and Thomas Pap
27. In vitro Matrigel Fibroblast Invasion Assay
Tanja C.A. Tolboom and Tom W.J. Huizinga
28. Culture and Analysis of Circulating Fibrocytes
Timothy E. Quan and Richard Bucala
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 468
Weight: 789g
Availability: Available
Subcategories: Genetics, Orthopaedics and Fractures
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