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Metastasis Research Protocols
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Main description:

In Volume I, Analysis of Cells and Tissues, we presented a range of protocols aimed at mapping and analyzing the expression of various molecules of pot- tial interest in metastasis research and for examining their production at the genetic level. In this second volume of metastasis research protocols, we move to the level of living cells and tissues and present methodologies applicable to examining metastatic behavior in vitro and in whole animal models. The methods described in the first section of this volume concentrate on the separation of cell lines with high and low metastatic potential, including the genetic modification of cell lines. The assay systems to test defined aspects of the metastatic cascade are then described in Part II and include cell migration assays, assays for matrix degrading enzymes, basement membrane degrading assays, adhesion assays, and assays of angiogenesis. The role of the specific elements of the metastatic cascade assayed in each of these systems in turn must of course be put into perspective relative to their roles in entire living organisms.

Back cover:

Although ninety percent of fatal cancer cases involve the spread of a primary tumor, the formation of metastases is still a poorly understood, complex process and a significant problem in the treatment of cancer patients. In Metastasis Research Protocols, leading international investigators describe in detail the key methods needed to investigate why and how metastasis occurs. Volume II of this two-volume set, Analysis of Cell Behavior In Vitro and In Vivo, presents a comprehensive collection of established and leading-edge techniques for examining metastatic behavior in vitro and in whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, along with assay systems to test defined aspects of the metastatic cascade. These systems include cell migration assays, assays for matrix degradation enzymes, basement membrane degradation assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models for metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis. The first volume of this set, Analysis of Cells and Tissues, focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression.

Comprehensive and authoritative, the two volumes of Metastasis Research Protocols constitute a gold-standard collection of readily reproducible methods for understanding the metastatic cascade-first at the cellular and molecular levels, then at the level of the whole organism-responsible for the spread of cancer and for developing novel strategies to combat its spread.


Part I. Selection/Segregation of Cell Populations for In vitro and In Vivo Assays of Metastatic Behavior

Cell Separations by Flow Cytometry
Derek Davies

Immunomagnetic Cell Separation
Catherine Clarke and Susan Davies

Genetic Modification of Cell Lines to Enhance Their Metastatic Capability
Daniel McWilliams and Hilary Collins

Part II. In Vitro Assays of Metastatic Behavior

Cell Aggregation Assays, Tom Boterberg
Marc E. Bracke, Erik A. Bruyneel, and Marc M. Mareel

Cell Migration and the Boyden Chamber
Nicholas S. Brown and Roy Bicknell

Quantification of Cell Motility: Gold Colloidal Phagokinetic Track Assay and Wound Healing Assay
Yasufumi Niinaka, Arayo Haga, and Avraham Raz

In Vitro Invasion Assay Using Matrigel®
Debbie M. S. Hall and Susan A. Brooks

Membrane Invastion Culture System
Mary J. C. Hendrix, Elisabeth A. Seftor, and Richard E. B. Seftor

Collagen Invasion Assay
Marc E. Bracke, Tom Boterberg, Erik A. Bruyneel, and Marc M. Mareel

Chick Heart Invasion Assay
Marc E. Bracke, Tom Boterberg, and Marc M. Mareel

Adhesion of Tumor Cells to Matrices and Endothelium
G. Ed Rainger

Assessment of Angiogenic Factors: The Chick Chorioallantoic Membrane Assay
Adam Jones, Chisato Fujiyama, Stephen Hague, and Roy Bicknell

Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth and Metastasis
Nerbil Kilic and Süleyman Ergün

Galectin-3 Binding and Metastasis
Pratima Nangia-Makker, Yuichiro Honjo, and Avraham Raz

Part III. Animal Models of Metastasis

Basic Principles for the Study of Metastasis Using Animal Models
Suzanne A. Eccles

The Chick Embryo Metastasis Model
Eric Petitclerc and Peter C. Brooks

Gene Transfection for Metastasis ResearchUsing Animal Models
Ruth J. Muschel and Jim Hua

Theoretical Considerations in Using Animal Models of Metastastis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice
Sue A. Watson and Teresa M. Morris

Xenograft Models in Immunodeficient Animals: I. Nude Mice: Spontaneous and Experimental Metastatis Models
Janet E. Price

Xenograft Models in Immunodeficient Animals: II. Use of SCID Mice in Metastasis Research: Breast and Colon Cancer Models of Metastasis
Udo Schumacher and Susan A. Brooks

Syngeneic Murine Metastasis Model: B16 Melanoma
Raffaella Giavazzi and Angela Garofalo

Transgenic Animal Models
Chantale Guy and Gizela Cordosa

Clinically Accurate Orthotopic Mouse Models of Cancer
Robert M. Hoffman

Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex Vivo
Marian Rocha, Volker Schirrmacher, and Victor Umansky

Green Fluorescent Protein for Metastastis Research
Robert M. Hoffman


ISBN-13: 9781617370991
Publisher: Springer (Humana Press)
Publication date: October, 2010
Pages: 332
Weight: 489g
Availability: POD
Subcategories: Oncology
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Average Rating 

This book is a valuable addition to the literature as it brings together key people in the field of cancer metastasis to provide practical step-by-step protocols. . . This book is written in such as way that it would be useful to beginning students and to anyone curious about the methods that are available to study the key steps in cancer metastasis. This book would also be useful to seasoned researchers as it brings together a number of different methodologies in one easy-to-read book. . . This is a very practical book covering areas of research in cancer invasion and metastasis. The greatest strength of the book is in collecting the different protocols that are useful in studying cellular behavior in one easy-to-read book.."-Weighted Numerical Score: 91 - 4 Stars!-Doody's Health Sciences Book Review Journal

"These volumes should be of interest for all medical and academic libraries where cancer research is performed." - E-Streams