Main description:

Visualization of chemicals in tissues has seen incredible advances in the past several years. Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging provides practical advice from experts in the field as well as an excellent overview of some of the most important recent advances in visualization. This timely volume explores topics from immunohistochemistry for multiple neurochemicals, detecting expression levels of neurochemicals, following cellular processes and ionic movement, identifying polysynaptic pathways subserving physiological responses to identifying functional changes in vivo. Written for the popular Neuromethods series, this work includes the kind of detailed description and implementation advice that is crucial for getting optimal results in the lab.

Meticulous and concise, Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging will prove invaluable for scientists seeking to gain a greater understanding of the practical skills, strengths, and pitfalls that these wonderful and exciting visualization techniques provide.

Contents:

1. Multiple Immunohistochemical Labelling of Peripheral Neurons

Ian L. Gibbins

2. Combined In Situ Hybridization and Immunohistochemistry in Tat Brain Tissue Using Digoxigenin Labelled Riboprobes

Natasha N. Kumar, Belinda R. Bowman and Ann K. Goodchild

3. In Situ Hybridization within the CNS Tissue: Combining In Situ Hybridization with Immunofluorescence

Dominic Bastien and Steve Lacroix

4. Visualizing GABAB Receptor Internalization and Intracellular Trafficking

Paola Ramoino, Paolo Bianchini, Alberto Diaspro, and Cesare Usai

5. Using Total Internal Reflection Fluorescence Microscopy (TIRFM) to Visualize Insulin Action

James G. Burchfield, Jamie A. Lopez, and William E. Hughes

6. Live-Cell Quantification of Mitochondrial Functional Parameters

Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman

7. Functional Imaging Using Two-Photon Microscopy in Living Tissue

Ivo Vanzetta, Thomas Deneux, Attila Kaszas, Gergely Katona, and Balazs Rozsa

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

Audrey Bonnan, Benjamin Grewe, and Andreas Frick

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

Ruth L. Stornetta

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

J. Patrick Card and Lynn W. Enquist

12. Visualization of Thermal Changes in Freely Moving Animals

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

Ian L. Gibbins

2. Combined In Situ Hybridization and Immunohistochemistry in Tat Brain Tissue Using Digoxigenin Labelled Riboprobes

Natasha N. Kumar, Belinda R. Bowman and Ann K. Goodchild

3. In Situ Hybridization within the CNS Tissue: Combining In Situ Hybridization with Immunofluorescence

Dominic Bastien and Steve Lacroix

4. Visualizing GABAB Receptor Internalization and Intracellular Trafficking

Paola Ramoino, Paolo Bianchini, Alberto Diaspro, and Cesare Usai

5. Using Total Internal Reflection Fluorescence Microscopy (TIRFM) to Visualize Insulin Action

James G. Burchfield, Jamie A. Lopez, and William E. Hughes

6. Live-Cell Quantification of Mitochondrial Functional Parameters

Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman

7. Functional Imaging Using Two-Photon Microscopy in Living Tissue

Ivo Vanzetta, Thomas Deneux, Attila Kaszas, Gergely Katona, and Balazs Rozsa

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

Audrey Bonnan, Benjamin Grewe, and Andreas Frick

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

Ruth L. Stornetta

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

J. Patrick Card and Lynn W. Enquist

12. Visualization of Thermal Changes in Freely Moving Animals

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

Natasha N. Kumar, Belinda R. Bowman and Ann K. Goodchild

3. In Situ Hybridization within the CNS Tissue: Combining In Situ Hybridization with Immunofluorescence

Dominic Bastien and Steve Lacroix

4. Visualizing GABAB Receptor Internalization and Intracellular Trafficking

Paola Ramoino, Paolo Bianchini, Alberto Diaspro, and Cesare Usai

5. Using Total Internal Reflection Fluorescence Microscopy (TIRFM) to Visualize Insulin Action

James G. Burchfield, Jamie A. Lopez, and William E. Hughes

6. Live-Cell Quantification of Mitochondrial Functional Parameters

Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman

7. Functional Imaging Using Two-Photon Microscopy in Living Tissue

Ivo Vanzetta, Thomas Deneux, Attila Kaszas, Gergely Katona, and Balazs Rozsa

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

Audrey Bonnan, Benjamin Grewe, and Andreas Frick

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

Ruth L. Stornetta

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

J. Patrick Card and Lynn W. Enquist

12. Visualization of Thermal Changes in Freely Moving Animals

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

Paola Ramoino, Paolo Bianchini, Alberto Diaspro, and Cesare Usai

5. Using Total Internal Reflection Fluorescence Microscopy (TIRFM) to Visualize Insulin Action

James G. Burchfield, Jamie A. Lopez, and William E. Hughes

6. Live-Cell Quantification of Mitochondrial Functional Parameters

Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman

7. Functional Imaging Using Two-Photon Microscopy in Living Tissue

Ivo Vanzetta, Thomas Deneux, Attila Kaszas, Gergely Katona, and Balazs Rozsa

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

Audrey Bonnan, Benjamin Grewe, and Andreas Frick

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

Ruth L. Stornetta

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

J. Patrick Card and Lynn W. Enquist

12. Visualization of Thermal Changes in Freely Moving Animals

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

James G. Burchfield, Jamie A. Lopez, and William E. Hughes

6. Live-Cell Quantification of Mitochondrial Functional Parameters

Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman

7. Functional Imaging Using Two-Photon Microscopy in Living Tissue

Ivo Vanzetta, Thomas Deneux, Attila Kaszas, Gergely Katona, and Balazs Rozsa

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

Audrey Bonnan, Benjamin Grewe, and Andreas Frick

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

Ruth L. Stornetta

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

J. Patrick Card and Lynn W. Enquist

12. Visualization of Thermal Changes in Freely Moving Animals

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman

7. Functional Imaging Using Two-Photon Microscopy in Living Tissue

Ivo Vanzetta, Thomas Deneux, Attila Kaszas, Gergely Katona, and Balazs Rozsa

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

Audrey Bonnan, Benjamin Grewe, and Andreas Frick

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

Ruth L. Stornetta

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

J. Patrick Card and Lynn W. Enquist

12. Visualization of Thermal Changes in Freely Moving Animals

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

Ivo Vanzetta, Thomas Deneux, Attila Kaszas, Gergely Katona, and Balazs Rozsa

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

Audrey Bonnan, Benjamin Grewe, and Andreas Frick

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

Ruth L. Stornetta

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

J. Patrick Card and Lynn W. Enquist

12. Visualization of Thermal Changes in Freely Moving Animals

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

Audrey Bonnan, Benjamin Grewe, and Andreas Frick

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

Ruth L. Stornetta

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

J. Patrick Card and Lynn W. Enquist

12. Visualization of Thermal Changes in Freely Moving Animals

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

Ruth L. Stornetta

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

J. Patrick Card and Lynn W. Enquist

12. Visualization of Thermal Changes in Freely Moving Animals

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

J. Patrick Card and Lynn W. Enquist

12. Visualization of Thermal Changes in Freely Moving Animals

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

J. Patrick Card and Lynn W. Enquist

12. Visualization of Thermal Changes in Freely Moving Animals

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

J. Patrick Card and Lynn W. Enquist

12. Visualization of Thermal Changes in Freely Moving Animals

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

Daniel M.L. Vianna and Pascal Carrive

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

Fernando Calamante

Fernando Calamante