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Main description:
This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.
Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.
Contents:
PART I: FFPE Sections
1Optimization of In Situ Hybridization and Co--Expression Analysis in the Central Nervous System
Madison Mikhail, Matteo Fassan, Louisa Mezache, Marco Chilosi, and Gerard J. Nuovo
PART II: Model Organisms
2 In Situ Hybridization Methods for RNA Visualization in C. elegans
Gina Broitman-Maduro and Morris F. Maduro
3 Multicolored Visualization of Transcript Distributions in Drosophila Embryos
Iris Soell and Giselbert Hauptmann
4 Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) in Whole-Mount Embryos
Jose Luis Ferran, Abdelmalik Ayad, Paloma Merchan, Nicanor Morales-Delgado, Luisa Sanchez-Arrones, Antonia Alonso, Juan Eugenio Sandoval, Sylvia Marianne Bardet, Ruben Corral-San-Miguel, Luis Oscar Sanchez-Guardado, Matias Hidalgo-Sanchez, Margaret Martinez-de-la-Torre, and Luis Puelles
5 Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) on Cryostat, Paraffin, or Floating Sections
Jose Luis Ferran, Abdelmalik Ayad, Paloma Merchan, Nicanor Morales-Delgado, Luisa Sanchez-Arrones, Antonia Alonso, Juan Eugenio Sandoval, Sylvia Marianne Bardet, Ruben Corral-San-Miguel, Luis Oscar Sanchez-Guardado, Matias Hidalgo-Sanchez, Margaret Martinez-de-la-Torre, and Luis Puelles
6 Combined Radioactive and Non-Radioactive Double In Situ Hybridization (DISH)
Jong-Hyun Son and Ursula H. Winzer-Serhan
7 One FISH, dFISH, three FISH: Sensitive Methods of Whole-Mount Fluorescent In Situ Hybridization in Freshwater Planarians
David D. R. Brown and Bret J. Pearson
8 Whole-Mount In Situ Hybridization and Immunohistochemistry in Xenopus Embryos
Edgar Pera, Helena Acosta, Nadege Gouignard, and Maria Climent
PART III: Systematic Gene Expression Analysis
9 Manual and Automated Whole-Mount In Situ Hybridization for Systematic Gene Expression Analysis in Embryonic Zebrafish Forebrain
Iris Soell and Giselbert Hauptmann
10 Large-Scale ISH on Mouse Brain Sections for Systematic Gene Expression Analysis in Developing Mouse Diencephalon
Tomomi Shimogori and Seth Blackshaw
11 High-Throughput In Situ Hybridization: Systematical Production of Gene Expression Data and Beyond
Lars Geffers and Gregor Eichele
PART IV: Evo-Devo
12 Analysis of Embryonic Gene Expression Patterns in the Hagfish
Yasuhiro Oisi, Osamu Kakitani, Shigeru Kuratani, and Kinya G. Ota
13 Gene Expression Analysis of Lamprey Embryos
Fumiaki Sugahara, Yasunori Murakami, and Shigeru Kuratani
14 Detection of mRNA and MicroRNA Expression in Basal Chordates, Amphioxus and Ascidians
Simona Candiani, Greta Garbarino, and Mario Pestarino
15 Analysis of Gene Expression in Neurons and Synapses by Multi-Color In Situ Hybridization
Leonid L. Moroz and Andrea B. Kohn
PART V: Neurons, Neuronal Compartments, And Projections
16 Fluorescent In Situ Hybridization in Primary Hippocampal Neurons to Detect Localized mRNAs
Jacki E. Heraud-Farlow, Tejaswini Sharangdhar, and Michael A. Kiebler
17 Combining Whole-Mount In Situ Hybridization with Neuronal Tracing and Immunohistochemistry
Jeremy S. Duncan, Karen L. Elliott, Jennifer Kersigo, Brian Gray, and Bernd Fritzsch
PART VI: miRNA Detection by LNA-FISH
18 Fluorescent In-Situ Hybridization Detection of MicroRNAs in Newt Tissue Cryosections
Jamie Ian Morrison
PART VII: Multiplex Fluorescent In Situ Hybridization (MP-FISH)
19 Subcellular Transcript Localization in Drosophila Embryos and Tissues Visualized by Multiplex FISH
Julie Bergalet, Carole Iampietro, Ashley Chin, Xuan-Tam Nguyen, Sulin Ore-Rodriguez, Neal Cody, and Eric Lecuyer
20 Application of Alkaline Phosphatase-Mediated Azo Dye Staining for Dual Fluorescent In Situ Hybridization in Zebrafish
Giselbert Hauptmann, Gilbert Lauter and Iris Soell
21 Multiplex Fluorescent RNA In Situ Hybridization via RNAscope
Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Kuang-Jung Chang, Allissa Nielson, Hong-Thuy Vo, Yuling Luo, and Xiao-Jun Ma
PART VIII: Quantum Dot-Based Methods (QD-FISH)
22 Quantum Dot-Based In Situ Hybridization and Immunohistochemistry to Detect mRNA and Protein at Subcellular Levels, Comparison with Studies Under Electron Microscopy
Akira Matsuno, Yudo Ishii, Mineko Murakami, Katsumi Hoya, Shoko M. Yamada, Shinya Miyamoto, Jae-Hyun Son, Hajime Nishido, So Yamada, Yasutaka Suzuki, Akira Teramoto, Hirotoshi Utsunomiya, Johbu Itoh, Susumu Takekoshi, R. Yoshiyuki Osamura, Akiko Mizutani, and Hiroko Okinaga
23 Quantitative Multiplexed Quantum Dot Based In Situ Hybridisation in Formalin Fixed Paraffin Embedded Tissue
Eleni Tholouli, Judith A. Hoyland, and Richard J. Byers
24 Single and Multiplexed Fluorescent In Situ Hybridization using Quantum Dots Nanocrystals in Xenopus
Andriani Ioannou, Iro Eleftheriou, Anna Charalambous, and Paris A Skourides
PART IX: Single Molecule mRNA Detection (SM-FISH)
25 Quantitative Single Molecule mRNA Fluorescent In Situ Hybridization in C. elegans
Remco A. Mentink, Ni Ji, Alexander van Oudenaarden, and Hendrik C. Korswagen
26 In Situ Detection of Individual mRNA Molecules with Padlock Probes and Target-Primed Rolling-Circle Amplification in Fixed Mouse Brain Tissues
Thomas Hauling, Rongqin Ke, Tomasz Krzywkowski, Marco Mignardi, and Mats Nilsson
27 Extended Multiplexed Fluorescent In Situ Hybridisation by Combinatorial Encoding of Individual Transcripts
Lars Martin Jakt and Satoko Moriwaki
PART X: Fast Fish
28 Characterizing Cellular Identity at One Cell Resolution
Amaresh K Ranjan, Mugdha V. Joglekar, and Anandwardhan A. Hardikar
29 Transglutaminase-Mediated In Situ Hybridization (TransISH) for mRNA Detection in Mammalian Tissues
Katsuyuki Miyawaki, Sumihare Noji, and Noriho Kamiya
30 ECHO-FISH for Gene Transcript Detection in Neuronal and Other Cells and Subcellular Compartments
Dan Ohtan Wang and Akimitsu Okamoto
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: February, 2015
Pages: 530
Weight: 652g
Availability: Available
Subcategories: Neuroscience
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