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MORE ABOUT THIS BOOK
Main description:
Efforts to measure neurotransmitters and related s- cies in living brain tissue by faradic electrochemistry began in earnest in the early 1970s. During the ensuing years, s- eral monographs and various symposia on the subject have appeared. The present volume of Neuromethods, Voltammetric Methods in Brain Systems, can be considered different from previous offerings in several respects. The methodology of in vivo electrochemistry has now reached a stable level of maturity. It is no longer necessary to convince interested re- ers that the techniques can measure, with specified degrees of reliability, certain neurotransmitters and metabolites in the extracellular fluid space. Moreover, the basic approaches of electroanalytical measurements are more widely appre- ated by neuroscientists and one need not explain in tutorial fashion the details of oxidative electrochemistry. Nevert- less, in keeping with the nature of the Neurumethods series, this is a "how to" volume and readers will find ample detail on the preparation of electrodes, practical experimental details, and the interpretation of results. But the emphasis is strongly on the application of voltammetric methods.
The first two chapters deal with fundamentals inherent in employing in vivo electrochemical measures. The first - ticle offers an authoritative account of our understanding of the surface states of carbon and carbon fiber electrodes. It provides a rationale for the various pretreatments that have allowed the "tuning" of electrode properties to obtain sen- tivity and fast response times.
Contents:
Carbon Electrode Surface Chemistry: Optimization of Bioanalytical Performance. Diffusion and Ion Shifts in the Brain Extracellular Microenvironment and Their Relevance for Voltammetric Measurements: The Brain Is Not A Beaker: In Vitro vs In Vivo Voltammetry. Fast Cyclic Voltammetry in Brain Slices. Rapid Chronocoulometric Measurements of Norepinephrine Overflow and Clearance in CNS Tissues. Monitoring Dopamine and Noradrenaline Release in Central and Peripheral Nervous Systems with Treated and Untreated Carbon-Fiber Electrodes. Regional Differences in Dopamine Release, Uptake, and Diffusion Measured by Fast-Scan Cyclic Voltammetry. The Measurement of Brain Ascorbate and Its Link with Excitatory Amino Acid Neurotransmission. Voltammetric and Amperometric Probes for Single-Cell Analysis. Measurement of the Time-Resolved Kinetics of Biogenic Amine Release and Transporter Activity by Rotating Disk Electrode Voltammetry In Vitro. Index.
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 368
Weight: 541g
Availability: Available
Subcategories: Neuroscience
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