(To see other currencies, click on price)
MORE ABOUT THIS BOOK
Main description:
The goal of the characterization and discovery of G protein-coupled receptors, arguably the most important class of signaling molecules in humans and other vertebrates, has spawned numerous vital methodologies. In Methods for the Discovery and Characterization of G Protein-Coupled Receptors, experts in the field present the very latest on the methods and technology used to characterize and discover novel mechanisms of GPCRs which, in many cases, can be used directly to design experiments for the reader’s particular GPCR of interest and their specific avenue of investigation. Divided into four convenient sections, this detailed volume covers GPCRs in the genome, trafficking of GPCRs, GPCRs on the membrane, as well as the regulation of these key receptors. Chapters also feature an important section called “Future Directions” which gives the reader an insight into advances soon to be realized in each area. Written for the popular Neuromethods series, this book contains the kind of detailed description and implementation advice that is crucial for getting optimal results.
Authoritative and cutting-edge, Methods for the Discovery and Characterization of G Protein-Coupled Receptors serves as an ideal guide for scientists determined to further our knowledge of crucially important set of receptors.
Feature:
Divided into four convenient parts, highlighting the life cycle of GPCRs
Features detailed methodologies for the characterization of GPCRs in a variety of research areas
Includes hints at the future directions of the key areas of study in this fast-moving field
Back cover:
The goal of the characterization and discovery of G protein-coupled receptors, arguably the most important class of signaling molecules in humans and other vertebrates, has spawned numerous vital methodologies. In Methods for the Discovery and Characterization of G Protein-Coupled Receptors, experts in the field present the very latest on the methods and technology used to characterize and discover novel mechanisms of GPCRs which, in many cases, can be used directly to design experiments for the reader’s particular GPCR of interest and their specific avenue of investigation. Divided into four convenient sections, this detailed volume covers GPCRs in the genome, trafficking of GPCRs, GPCRs on the membrane, as well as the regulation of these key receptors. Chapters also feature an important section called “Future Directions” which gives the reader an insight into advances soon to be realized in each area. Written for the popular Neuromethods series, this book contains the kind of detailed description and implementation advice that is crucial for getting optimal results.
Authoritative and cutting-edge, Methods for the Discovery and Characterization of G Protein-Coupled Receptors serves as an ideal guide for scientists determined to further our knowledge of crucially important set of receptors.
Contents:
Part I: The G Protein-Coupled Receptor in the Genome
1. In Silico Identification of Novel G Protein-Coupled Receptors
Matthew N. Davies, David E. Gloriam, and Darren R. Flower
2. Alternative Pre-mRNA Splicing of G Protein-Coupled Receptors
Ying-Xian Pan, Steven Grinnell, and Gavril W. Pasternak
3. Detecting Polymorphisms in G Protein-Coupled Receptor Genes
Dmitri Proudnikov, Vadim Yuferov, and Mary Jeanne Kreek
4. Regulation of the Transcription of G Protein-Coupled Receptor Genes
Cheol Kyu Hwang, Ping-Yee Law, Li-Na Wei, and Horace H. Loh
5. Deciphering the Evolution of G Protein-Coupled Receptors in Vertebrates
Craig W. Stevens
Part II: The Trafficking of G Protein-Coupled Receptors
6. A New Approach to Visualize Endogenously Expressed G Protein-Coupled Receptors in Tissues and Living Cells
Brigitte L Kieffer and Grégory Scherrer
7. Post-Translational Regulation of G Protein-Coupled Receptors
Yu Qiu and Ping-Yee Law
8. Discovering G Protein-Coupled Receptor Motifs Mediating Export from the Endoplasmic Reticulum
Chunmin Dong and Guangyu Wu
9. Identifying G Protein-Coupled Receptor Escorts, Chaperones, and Intracellular Tethers Regulating Receptor Density at the Cell Surface
Stefano Marullo, Liliana Pardo Lopez, and Lamia Achour
10. A Novel Method for Determining the Kinetics of G Protein-Coupled Receptor Plasma Membrane Expression
Gregory W. Sawyer
Part III: The G Protein-Coupled Receptor on the Membrane
11. Characterizing the Pharmacology of G Protein-Coupled Receptors in Transfected Cell Lines
Kathryn A. Seely and Paul L. Prather
12. Novel Assay Technologies for the Discovery of G Protein-Coupled Receptor Drugs
Elisa Alvarez-Curto, Richard J. Ward, and Graeme Milligan
13. Discovering Cell-Type-Specific Patterns of G Protein-Coupled Receptor Phosphorylation
Kok Choi Kong, Andrew B. Tobin, and Adrian J. Butcher
14. Quantifying Allosteric Modulation of G Protein-Coupled Receptors
Frederick J. Ehlert and Hinako Suga
15. Experimental Designs for the Study of Receptor-Receptor Interactions
Dennis Paul
Part IV: The Regulation of G Protein-Coupled Receptors
16. Elucidating Agonist-Selective Mechanisms of G Protein-Coupled Receptor Desensitization
Chris P. Bailey and Eamonn Kelly
17. Detecting the Role of Arrestins in G-Protein Coupled Receptor Regulation
Laura M. Bohn and Patricia H. McDonald
18. Characterizing Molecular Mobility and Membrane Interactions of G Protein-Coupled Receptors
Vladana Vukojević, Yu Ming, and Lars Terenius
19. Using RNA Interference to Downregulate G Protein-Coupled Receptors
Philippe Sarret, Louis Doré-Savard, Pascal Tétreault, Valérie Bégin-Lavallée, Marc-André Dansereau, and Nicolas Beaudet
20. Upregulating G Protein-Coupled Receptors with Receptor Antagonists
Ellen M. Unterwald
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: July, 2011
Pages: 420
Weight: 1022g
Availability: Not available (reason unspecified)
Subcategories: Genetics, Neuroscience
From the same series
CUSTOMER REVIEWS
From the reviews:
“Methods for the Discovery and Characterization of G Protein-Coupled Receptors is an excellent reference text for anyone concerned with the dynamic life of GPCRs in living tissues. The book is suitably organized to cater to a wide audience, and the material is presented in a logical framework to permit understanding of major concepts by a novice. … This would be a particularly valuable book for any structural biologist looking to build complexity into their experimental system to account for in vivo phenomena.” (Michelle O’Malley, ChemBioChem, Vol. 13 (6), April, 2012)