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Main description:
This volume presents state-of-the-art methods for reliable detection of epigenetic changes in the nervous system. Epigenetic Methods in Neuroscience Research guides readers through methods for the analyses of chromatin remodeling, transposable elements, non-coding RNAs, such as miRNAs, and circadian oscillations, including: analysis of DNA methylation in neuronal and glial cells or small tissue samples; sensitive method for quantification of alternative methylated forms of cytosines by liquid chromatography/mass spectrometry; affinity-based detection of modified cytosines by immunohistochemistry or methylated DNA immunoprecipitation; chromatin immunoprecipitation, or ChIP; miRNA high-throughput profiling and the in situ detection of miRNA subtle expression in the brain; analysis of genes with alternative 3'UTRs; and the cite-specific delivery of chromatin-modifying drugs. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.
Concise and easy-to-use, Epigenetic Methods in Neuroscience Research provides multidisciplinary epigenetic approach to study genome, and neural, plasticity that will help the reader to successfully address the challenges associated with neurodevelopmental, psychiatric, and neurodegenerative disorders.
Contents:
Preface
Series Preface
Contributors
PART I INTRODUCTION
1 Epigenetics: From Basic Biology to Chromatin Modifying Drugs and New Potential Clinical Applications
Francesco Matrisciano, Isabella Panaccione, Erbo Dong, Dennis R Grayson, and Alessandro Guidotti
2 Analysis of Brain Epigenome: A Guide To Epigenetic Methods
Nina N. Karpova
PART II ANALYSIS OF CHROMATIN REMODELING
3 Analysis of DNA Methylation by Bisulfite Reaction in Neural Cells as an Example of Orexin Neurons
Koji Hayakawa, Mitsuko Hirosawa, and Kunio Shiota
4 Protocol for The Simultaneous Analysis of Gene DNA Methylation and mRNA Expression Levels in the Rodent Brain
Juzoh Umemori and Nina N. Karpova
5 Simultaneous Quantification Of Global 5mc And 5hmc Levels In The Nervous System Using A HPLC/MS Method
Daniel L. Ross and Garrett A. Kaas
6 Protocol for Methylated DNA Immunoprecipitation (Medip) Analysis
Nina N. Karpova and Juzoh Umemori
7 Cell Type-Specific DNA Methylation Analysis in Neurons and Glia
Miki Bundo, Tadafumi Kato, and Kazuya Iwamoto
8 Immunohistochemical Detection of Oxidized Forms of 5-Methylcytosine in Embryonic and Adult Brain Tissue
Abdulkadir Abakir, Lee M. Wheldon, and Alexey Ruzov
9 Analysis of Histone Modifications in Neural Cells as an Example of Orexin Neurons
Koji Hayakawa, Chikako Yoneda, Ruiko Tani, and Kunio Shiota
10 Site-Specific Delivery of Epigenetic Modulating Drugs into the Rat Brain
Amanda Sales, Caroline Biojone, and Samia Joca
PART III ANALYSIS OF NON-CODING GENOME
11 Mammalian Genome Plasticity: Expression Analysis Of Transposable Elements
Brian B. Griffiths and Richard G. Hunter
12 Tools for Analyzing the Role Of Local Protein Synthesis in Synaptic Plasticity
Lauren L. Orefice and Baoji Xu
13 In Situ Detection Of Neuron-Specific Micrornas in Frozen Brain Tissue
Asli Silahtaroglu, Silke Herzer, and Bjoern Meister
14 Protocol For High-Throughput Mirna Profiling Of The Rat Brain
Sharon L. Hollins, Fredrick R. Walke, and Murray J. Cairns
PART IV ANALYSIS OF CIRCADIAN OSCILATIONS
15 Metabolites as Clock Hands: Estimation of Internal Body Time Using Blood Metabolomics
Hitoshi Iuchi, Rikuhiro G. Yamada, and Hiroki R. Ueda
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2015
Pages: 212
Weight: 7199g
Availability: Available
Subcategories: Genetics, Neuroscience
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