(To see other currencies, click on price)
MORE ABOUT THIS BOOK
Main description:
E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal. This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique.
Contents:
Technology of Patch-Clamp Electrodes. Whole-Cell Patch-Clamp Recordings. Pressure/Patch-Clamp Methods. Cell-Free Ion-Channel Recording. Perfusion of Patch Pipets. Concentration Clamp Techniques. Perforated Patch-Clamp Techniques. The Loose Patch Voltage Clamp Technique. Patch-Clamp Recording and RT-PCR on Single Cells. Patch-Clamp Technique in Brain Slices. Xenopus Oocyte Microinjection and Ion-Channel Expression. Index.
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2013
Pages: 316
Weight: 493g
Availability: Available
Subcategories: Neuroscience
From the same series
Francisco Ciruela
Alon Korngreen
Judee K. Burgoon
David Walker
Nina Karpova
Gabriela K. Popescu
Jerome Y. Yager
Heather A. Bimonte-Nelson
Tetsuichiro Saito
Adalberto Merighi
Giselbert Hauptmann
Joost Verhaagen
Pierre L. Roubertoux
Kewal K. Jain
Wolfgang Blenau
Mario Tiberi
Vangelis Sakkalis
Benjamin R. Arenkiel
Jennie B. Leach
Johannes Hirrlinger
Alvaro Pascual-Leone
Theodore H. Schwartz
Eugenio F. Fornasiero
Antoine Triller
Fritjof Helmchen
Alan A. Boulton
Alan A. Boulton
Peter Thorn
Riccardo Brambilla
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Wolfgang Walz
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Alan A. Boulton
Peter V. Nguyen
Stephane Marinesco
H. Aldskogius
Rolf Dermietzel
Paul M. Pilowsky
Giuseppe Di Giovanni
Ricardo Martinez Murillo
Nicole M. Avena
Klaus Ballanyi
Jean-Rene Martin
Bassem A. Hassan
Gerhard Grunder
Emilio Badoer
Allan V. Kalueff
Hideyuki Mukai
Todd D. Gould
Ole H. Petersen
Tommaso Fellin
Alexei Morozov
Yannis Karamanos
Alan A. Boulton
Stephen B. Dunnett
Stephen B. Dunnett
Todd D. Gould
Patricio O'Donnell
Ka Wan Li
Scott Q. Harper
Michael Aschner
James J. Chambers
Mary C. Olmstead
Illana Gozes
Peter Paul de Deyn
Robert P. Vertes
Andrea C. LeBlanc
Shlomo Seidman
Hugh C. Hemmings
Alan A. Boulton
Alan A. Boulton
Wolfgang Walz
Alan A. Boulton
Allan V. Kalueff
Allan V. Kalueff
Chao Ma
Jacob Raber
Ulrich Dirnagl
Iok-Hou Pang
Allan V. Kalueff
Kewal K. Jain
Prof. Alexei Verkhratsky
Todd D. Gould
Massimo Filippi
Scott C. Baraban
Illana Gozes
Wolfgang Walz