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Main description:
Electrophysiological methods are used at nearly all levels of biological research today. From basic science investigation to bringing new drugs to market, electrophysiology is an indispensable research technique that provides essential insights into cellular and molecular functions. This work is intended to be a practical guide for understanding and using contemporary electrophysiological manipulations and assays in brain research. New researchers attempting to learn electrophysiological techniques are confronted with a lack of adequate guiding resources. Currently, most electrophysiology-based research knowledge is passed on by experienced individuals to others within the same laboratory. But, this master-apprentice scenario has many inherent difficulties, as it is both a time intensive process and also leads to incomplete knowledge transfer, or even superstitious and maladaptive practices. Few external guiding resources exist for a budding researcher who wishes to gain the skills necessary to conduct responsible and competent electrophysiology research. Dr. Yan Dong, an expert in utilizing electrophysiological techniques for brain research with fifteen years of experience, and Peter Neumann hope this work can act as a concise and practical aid to remedy the current situation. This book will cover both basic methods and advanced techniques clearly and in depth, as well as the essential background and theory necessary to understand the methods. Additionally, this book also covers conventional and contemporary patch-clamp methods, experimental design, data collection and analysis, common pitfalls to avoid, important nuances, technical concerns, practical applications, and advice for teaching new researchers. Together, this represents a contemporary best-practices guide for collecting and interpreting electrophysiological data for brain research.
Contents:
Overview: Electrophysiology: Strength and Limitation
Part One: Basic Concepts
Chapter 1: Extracellular and intracellular recordings
Chapter 2: Electrical theory
Chapter 3: Amplifiers
Chapter 4: Salt environment
Chapter 5: Electrodes
Chapter 6: Spatiotemporal effects of synaptic current
Chapter 7: Perforated patch
Part Two: Recording of synaptic current
Chapter 8: Isolation of synaptic current
Chapter 9: Fast and slow synaptic currents
Chapter 10: Measuring kinetics of synaptic current.
Chapter 11: Measuring presynaptic release probability
Chapter 12: Long-term measurements
Chapter 13: Measuring reversal potentials
Part Three: Basic Experimentations of synaptic transmission
Chapter 14: Amplitude
Chapter 15: Pre vs. post synaptic effect
Chapter 16: Runup and rundown
Chapter 17: Kinetics of synaptic current
Part Four: Experimentations with computational components
Chapter 18: Measurement of a single synapse
Chapter 19: Measurement of silent synapses
Chapter 20: Dendritic patch
Part Five: Experimentations with molecular and visual components
Chapter 21: Electrophysiological and visual tags
Part Six: In vivo Recordings
Chapter 22: Extracellular recordings
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2015
Pages: 195
Weight: 6733g
Availability: Available
Subcategories: Neuroscience
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