Every year new laboratory methods are developed for the identification and classification of infectious disease agents. The number of new approaches to aid epidemiological studies of microbial pathogens is continuously growing. New biotechnology improvements have played a key role in leading to new findings in the fields of laboratory diagnostic and classification of important etiological agents. For each bacterial species, a number of different methods can be used to identify the organism and determine its possible relationship to an epidemic- or outbreak-related clone. Because of the array of different laboratory methods, it is often difficult to choose an effective low-cost method that could be used routinely in laboratories. The need to establish more sensitive methods based on molecular analysis of the microorganism genome has led many laboratories to make large investments in order to achieve results which could have been reached with minor expenses.
Molecular Typing in Bacterial Infections covers common bacterial pathogenic agents, with the most effective methods for their identification and classification in the light of their specific epidemiology. The book will be a valuable resource for molecular typing of infectious diseases agents encountered in both the research and hospital clinical lab settings, as well as culture collections. Each chapter provides an overview of molecular approaches to typing bacterial pathogens. Part I gives a general overview of typing methods used in the traditional microbiology laboratory in comparison to molecular methods of epidemiology. In Part II, the relative strengths and weaknesses of the different methods applicable to the specific agents of infectious diseases are emphasized. Specific emphasis is placed on recent changes and updates in molecular typing.
A valuable resource for molecular typing of infectious diseases agents
Useful in both research and hospital clinical lab settings
Methods discussed can be used to differentiate bacterial pathogens and identify linkage to outbreaks
The accurate identification and typing of microbes is essential for researchers in all fields of microbiology. The investigation of species diversity is crucial for the determination of the genetic relatedness of isolates for epidemiological studies. The development of molecular genotyping methods has improved the classification and typing of microorganisms at the sub-species level. In Molecular Typing in Bacterial Infections, readers will find an ultimate guide to molecular methods for the classification and typing of most human bacterial pathogens, covering a wide range of techniques which can be easily applied to the investigation of infectious diseases.
The emphasis is on nucleic acid-based assays and alternative biochemically and immunologically-based formats providing significant potential improvement of typing technologies that are transforming the field of diagnostic testing.
Comprehensive and practical, Molecular Typing in Bacterial Infections provides state-of-the-art methods not only for accurate diagnostic, but also for the correct classification of different types which will prove to be critical in unraveling the routes of spread of human pathogens.
Section 1: General consideration on microorganism typing methods.- Molecular epidemiology.- Section 2: Gastrointestinal Pathogens.- Enterococci.- Listeria monocytogenes.- Enterobacteriaceae.- Vibrio cholerae.- Clostridium difficile.- Oral and Intestinal Bacteroidetes.- Section 3: Oral and Respiratory Pathogens.- Streptococci.- Streptococcus mutans.- Periodontal anaerobic bacteria.- Nontuberculous mycobacteria.- Neisseria meningitidis.- Haemophilus influenzae.- Moraxella.- Legionella pneumophila.- Mycoplasma and Ureaplasma.- Corynebacterium diphtheriae.- Burkholderia.- Section 4: Urogenital Pathogens.- Treponema.- Chlamydiaceae.- Section 5: Vector Borne Pathogens.- Borreliae.- Erysipelothrix.- Section 6: Pathogens causing Healthcare-Associated Infection.- Staphylococci.- Pseudomonas.- Section 7: Emerging and Reemerging Pathogens.- Acinetobacter baumannii.- Coxiella burnetii.- Index.