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Physical Methods on Biological Membranes and Their Model Systems
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Main description:

This book examines in depth the theory, instrumental innova- tions, potential applications and limitations of the different techniques -- NMR, EPR, DSC, spectrofluorimetry, and electron microscopy -- that are being used to investigate the function and structure of biological membranes and their model systems. Pro- viding an overview of the present status of research, this book gives evidence of the complementary nature of information generated by different techniques, reminding one of the necessity to create a common language and viewpoint of membranes. F. Conti W. E. Blumberg J. De Gier F. Pocchiari v CONTENTS Physical methods on biological membranes and their model system: A perspective . * * * * * . * * * * * * . * 1 F. Conti, J. de Gier and W. E. Blumberg High resolution NMR spectroQcopy in liquids and solids * * 5 T. Axenrod Basic principles of deuterium and phosphorus-31-magnetic resonance 27 J. Seelig Use of electron spin resonance to study complex biological membranes . . ***. *********. *. * 39 H. M. Swartz Freeze-etching electron microscopy: recent developments and application to the study of biological membranes and their components. * * . * * . * * * * . * * ** 55 T.
Gu1ik-Krzywicki The nature of intramembraneous particles * * * * * * * *. 73 A. J. Verk1eij Lateral and polymorphic phase transitions in relation to the barrier function of a lipid membrane. * * * . * 81 J. de Gier, C. J. A. van Echte1d, J. A.


Contents:

Physical methods on biological membranes and their model system: A perspective.- High resolution NMR spectroscopy in liquids and solids.- Basic principles of deuterium and phosphorus-31-magnetic resonance.- Use of electron spin resonance to study complex biological membranes.- Freeze-etching electron microscopy: recent developments and application to the study of biological membranes and their components.- The nature of intramembraneous particles.- Lateral and polymorphic phase transitions in relation to the barrier function of a lipid membrane.- Fluorescence energy transfer as a structural probe in membranes and membrane in bound proteins.- The interaction of intrinsic proteins and lipids in biomembranes.- Conformational studies of membrane proteins by high resolution NMR.- Water organization in reversed micelles.- Lipid-protein interactions: from crystalline lipoproteins to intact membranes.- Membrane fusion and lipid polymorphism.- Control of membrane fusion by divalent cations, phospholipid head-groups and proteins.- Correlation between steady-state and time-resolved fluorescence anisotropy data.- Membrane lipid fluidity at a physiologically relevant scale: determination by fluorescence polarization.- Dynamic interaction of fluorescently labeled adenosine deaminase in intact human erythrocytes: relation to abnormal activity in malignant cells.- Phase separation in phospholipid mixtures by fluorescence anisotropy.- Membrane fluidity and lipid composition.- Membrane fluidity and membrane activities.- Membrane fluorescence anisotropy behaviour during cell cycle..- Stable biomembrane surfaces formed by phospholipids polymers.- Utilizing mast cells and basophils to study lipoxygenase involvement in membrane activation.- Immunoglobulin surface adsorption studied by total internal reflection with fluorescence correlation spectroscopy.- Enthalpy state of different liposome preparations of dimyristoyl phosphatidylcholine and their stability in the presence of a complex forming protein.- Free-flow electrophoresis as a suitable tool to explore intervesicular interactions.- Solid Atate 19F NMR in "Model" membrane systems.- Organized cultures of nerve tissues: a novel model system for studies of lipid protein interaction on the functional level.- Partition of proteins between water and non-polar phases.- Photomodification of Biomembranes.


PRODUCT DETAILS

ISBN-13: 9781468475401
Publisher: Springer (Springer-Verlag New York Inc.)
Publication date: May, 2012
Pages: None
Weight: 891g
Availability: Available
Subcategories: Biochemistry
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