In recent years several different gene silencing phenomena have been discovered in plants. The book summarizes the most recent data on gene silencing phenomena such as trans-, inactivation, paramutation and co-suppression. Plant researchers will find this edition a valuable help in differentiating between a number of puzzling and partly contradictory gene silencing events. Those not familiar with plant molecular biology are introduced into the relevant methods and scientific models. In addition examples and models of gene silencing in flamentous fungi, Drosophila and mammalian systems are presented. By providing a comparative update on gene silencing effects in different eukaryotes, this book should stimulate communication among scientists working in diverse areas of eukaryotic gene regulation.
List of Contents.- trans-Inactivation of Homologous Sequences in Nicotiana tabacum.- DNA Methylation and Transgene Silencing in Petunia hybrida.- Transgene Inactivation in Arabidopsis thaliana.- Developmental Regulation of Co-suppression in Petunia hybrida.- Suppression of Flavonoid Flower Pigmentation Genes in Petunia hybrida by the Introduction of Antisense and Sense Genes.- Sense and Antisense Inactivation of Fruit Ripening Genes in Tomato.- Co-suppression of ?-1 ,3-Glucanase Genes in Nicotiana tabacum.- Gene Silencing in Transgenic Plants: A Heuristic Autoregulation Model.- Paramutation in Maize and Related Allelic Interaction.- DNA Methylation and Activity of the Maize Spm Transposable Element.- Genetic and Epigenetic Inactivation of Repetitive Sequences in Neurospora crassa: RIP, DNA Methylation, and Quelling.- MIP: An Epigenetic Gene Silencing Process in Ascobolus immersus.- Gene Silencing in Drosophila.- Uptake of Foreign DNA by Mammalian Cells Via the Gastrointestinal Tract in Mice: Methylation of Foreign DNA-A Cellular Defense Mechanism.