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Peptide Arrays on Membrane Supports
Synthesis and Applications
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Main description:

Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding.
The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.


Feature:

Detailed step-by-step protocols, including troubleshooting sections and many hints and comments from the authors
The technology described is ideally suited to cope with the flood of data derived from the Human Genome Project
The protocols are well established and are provided by authors with plenty of experience with this technology


Contents:

1 SPOT Synthesis — Scope of Applications.- 2 Chemistry of Fmoc Peptide Synthesis on Membranes.- 3 Manual Peptide Synthesis.- 4 Automated Synthesis of Solid-Phase Bound Peptides.- 5 Epitope Mapping of Antibodies with Solid-Phase Oligopeptides.- 6 Protein-Protein Interactions.- 7 Analysis of Protein-DNA Interactions.- 8 Affinity Purification and Competition Assays Using Solid-Phase Oligopeptides.- 9 Mutational Analysis and Structure Predictions.- 10 Modification of Immobilized Peptides.- 11 Immobilized Peptides to Study Protein-Protein Interactions — Potential and Pitfalls.- Abbreviations.


PRODUCT DETAILS

ISBN-13: 9783642076398
Publisher: Springer (Springer Berlin Heidelberg)
Publication date: October, 2010
Pages: 184
Weight: 290g
Availability: POD
Subcategories: Biochemistry, General Issues, Pharmacology, Physiology
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From the reviews:

"In this book the authors provide a detailed overview of the technology, including insights into basic chemistry and established applications. All protocols have been written by experienced researchers in SPOT technology and have therefore been optimized for practical use. … It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding." (New Drugs, Issue 7, October, 2002)

"Detailed protocols for peptide synthesis and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but potentially also for diagnostic and therapeutic applications … . This book provides a detailed overview of the technology, including insights into the basic chemistry and established applications. ... The book is suitable for researchers in basic research and in therapeutic applications as well as for advanced students." (Biotech International, Vol. 15 (2), 2003)