Main description:

The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories.
A Springer Lab Manual Review of the First Edition:
"This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended."
CYTOBIOS

Contents:

Preface - Leonard A. Herzenberg; Part I Flow Cytometry: Operation of a Flow Cytometer - Christoph Goettlinger, Birgit Mechtold and Andreas Radbruch; Part II Immunofluorescence: 2 Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies - Werner Muller; 3 Immunofluorescence: Basic Considerations - Andreas Radbruch; 4 Multicolor Immunofluorescence Analysis - Chia-Huei Chen, Kerstin Willmann, Dieter Recktenwald; 5 Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells - Mario Assenmacher; 6 Cytometry and Sorting of Live Cells According to Their Secreted Products - Rudolf A. MANZ; 7 Cytometry of Rare Surface Molecules by Magnetofluorescent Liposomes - Alexander Scheffold; 8 Scatchard Analysis by Flow Cytometry - Robert F. Murphy; 9 Microbead Assay for Quantification of Neuronal Adhesion Molecule Interaction by Flow Cytometry - Attila Trnok, Ursel Noehrenberg, Hans-Jurgen Vollmer, Stephan Schuhmacher; Part III DNA and Proliferation: 10 Preparation and Staining of Fixed Cells for High-Resolution DNA Analysis - Friedrich J. Otto; 11 Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression - Werner Muller; 12 High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique - Manfred Kubbies; Part IV Cellular Activation and Biochemistry: 13 Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium- Manfred Kubbies; 14 Detection and Purification of Rare Responders by Fixed-Time Flow Cytometry - Attila Tarnok and Henning Ulrich; 15 Biochemical Parameters of Cell Function - Gregor Rothe and Gunther Valet; 16 The FACS-Gal Assay - Steffen Jung; 17 Ligand Acidification by Nonadherent Cells - Robert F. Murphy; Part V Cell Sorting: 18 Powerful Preselection - Charlotte Esser; 19 Magnetic Selection of Transfected Cells - Gregor Siebenkotten and Ute Behrens-Jung; 20 High Gradient Magnetic Cell Sorting - Stefan Miltenyi andJurgen Schmitz; 21 Setup of a Flow Sorter - Christoph Goettlinger, Birgit Mechtold, Klaus Meyer and Andreas Radbruch; 22 Sorting of Rare Cells - Walter Weichel, Sigrid Irlenbusch, Kimitaka Kato and Andreas Radbruch; 23 Fluorescence-Activated Chromosome Sorting - Judith A. Fantes and Daryll K. Green; 24 Analysis and Sorting of Plant Chromosomes by Flow Cytometry - Jacky Veuskens, Dominque Marie, Steph Hinnisdaels, Spencer C. Brown; 25 Large Particle Sorting - David W. Galbraith and Sergio Lucretti; Part VI Saftey: 26 Biological and Laser Safety - Klaus L. Meyer; Appendices: Glossary; Suppliers; Cytometry on the Internet