Main description:

Analytical chemists and materials scientists will find this a useful addition to their armory. The contributors have sought to highlight the present state of affairs in the validation and quality assurance of fluorescence measurements, as well as the need for future standards. Methods included range from steady-state fluorometry and microfluorometry, microscopy, and micro-array technology, to time-resolved fluorescence and fluorescence depolarization imaging techniques.

Contents:

Fluorescence Microscopy: Need for Standardization of Fluorescence Measurements from the Instrument Manufacturer's View.- Characterization and Calibration in Wide Field and Sectioned Fluorescence Microscopy SIPcharts.- Quantitative Fluorescence Microscopy: Considerations and Controls.- Comparability of Fluorescence Microscopy Data and Need for Instrument Characterization of Spectral Scanning Microscopes.- Fluorescence Lifetime Imaging Microscopy: Quality Assessment and Standards.- Single Molecule Spectroscopy: State of the Art and Novel Trends in Fluorescence Correlation Spectroscopy.- Single Molecule Spectroscopy: Instrumentation and Multiparameter Detection.- Fluorescence-Based Microarray Technology: Applications, Future Trends, and the Need for Standardization.- Comparability of Microarray Experiments from the Instrument and the Sample Site and Approaches Towards Standardization.- Microarray Technology: Unresolved Issues and Future Challenges from a Regulatory Perspective.- Protein Arrays and Fluorescence Detection: Applications and Limitations.- Flow Cytometry: Flow Cytometry: Instrumentation, Applications, Future Tends and Limitations.- Flow Cytometry Quality Assurance.- Approaches to Quantitation in Flow Cytometry.- Fluorescence Immunoassays: Immunoassays: Basic Concepts, Physical Chemistry and Validation.- Time-Resolved Fluorometric Immunoassays: Instrumentation, Applications, Unresolved Issues and Future Trends.- Particle-Based Assays: Applications and Unresolved Issues.- Advances in Fluorescence Enzyme Detection Methods.- Quantitative PCR: Quantitative Real-Time PCR: Fluorescent Probe Options and Issues.- Fluorescence in Situ Hybridization and Immunohistochemistry: Cellular Bioimaging in Fluorescent Cancer Biomarker Evaluation: Validation, Technologies and Standards Development.- Fluorescence Technologies in Biomedical Diagnostics: Fluorescence Techniques in Biomedical Diagnostics: Instrumentation, Analysis and Unresolved Issues.- In-vivo FluorescenceImaging: Applications, Future Trends and Approaches to Standardization.