Main description:

The key element of any fluorescence sensing or imaging technology is the fluorescence reporter, which transforms the information on molecular interactions and dynamics into measurable signals of fluorescence emission. This book, written by a team of frontline researchers, demonstrates the broad field of applications of fluorescence reporters, starting from nanoscopic properties of materials, such as self-assembled thin films, polymers and ionic liquids, through biological macromolecules and further to living cell, tissue and body imaging. Basic information on obtaining and interpreting experimental data is presented and recent progress in these practically important areas is highlighted. The book is addressed to a broad interdisciplinary audience.

Feature:

A comprehensive review

Written by experts

With numerous colour illustrations

Contents:

Part I   General aspects

 Interfacial behavior of fluorescent dyes. Power and weakness of nanoscopic description
Alexander P. Demchenko and Semen O. Yesylevskyy

 Part II   Probing condensed media 

Fluorescence probing of physicochemical characteristics of the room temperature ionic liquids
Anunay Samanta

 Fluorescence spectroscopy in polymer science
Tanzeela N. Raja and Albert M. Brouwer 

Fluorescence probing in structurally anisotropic materials. From liquid crystals to macromolecules, micelles and lipid bilayers
Semen O. Yesylevskyy and Alexander P. Demchenko

Part III   Fluorescence reporters in biosensing

Optimized dyes for protein and nucleic acid detection
Sergiy M.Yarmoluk, Vladyslava B. Kovalska, Kateryna D.Volkova

 Functional nucleic acids for fluorescence based biosensing applications
Jennifer Lee, Lawrence Lin, and Yingfu Li 

Part IV   Cell imaging with organic dyes 

Covalent labeling of biomolecules in living cells
Tilman Plass and Carsten Schultz 

Tetracysteine and bipartite tags for biarsenical organic fluorophores
Carla Spagnuolo, Maria Joselevich, Federico Coluccio Leskow and Elizabeth A. Jares-Erijman 

Labeling of oligohistidine-tagged proteins
Jacob Piehler

 Part V   Tissue and whole body imaging 

In vivo imaging of vascular targets using near-infrared fluorescent probes
Jan Klohs and Markus Rudin

 Whole-body imaging of hematopoietic and cancer cells using near infrared probes
Vyacheslav Kalchenko, Michal Neeman and Alon Harmelin