Main description:

This volume explores label-free biosensors, advantageous in part because this technology bypasses the need of labels, reporters, and cell engineering, all of which are common to labeled techniques but may introduce artifacts in assay results. Addressing several fundamental and practical aspects as to how to implement label-free methods in the drug discovery process, this book covers a wide range of topics, including binding kinetics determination, fragment screening, antibody epitope mapping, protein-protein interaction profiling and screening, receptor pathway deconvolution, drug pharmacology profiling and screening, target identification, drug toxicity assessment, and physical phenotype profiling and diagnostics based on various cellular processes such as cell adhesion, migration, invasion, infection, and inflammation. As part of the Methods in Pharmacology and Toxicology series, chapters aim to provide key detail and implementation advice to aid with progress in the lab.

Practical and thorough, Label-Free Biosensor Methods in Drug Discovery provides a new avenue for rapid access to a focused collection of highly regarded contributions in the field.

Contents:

Part I: Review

1. Label-Free Technologies: Which Technique to Use and What to Watch Out For!

Reena Halai and Matthew Cooper

2. Label-Free Cell Phenotypic Profiling and Screening: Techniques, Experimental Design, and Data Assessment

Ye Fang

3. Surface Plasmon Resonance for Therapeutic Antibody Characterization

S. Nicole Davidoff, Noah T. Ditto, Adam R. Miles, Josh Eckman, and Benjamin D. Brooks

4. Label-Free Cell-Based Biosensor Methods in Drug Toxicology Analysis

Jie Zhou, Xianxin Qiu, and Ping Wang

Part II: Biochemical Profiling and Screening

5. Kinetics Characterization of Ligand-Receptor Interactions Using Oblique-Incidence Reflectivity Difference Method

Shuang Liu, Guozhen Yang, Huibin Lu, and Heng Zhu

6. Label-Free Inhibition in Solution Assays for Fragment Screening

Stefan Geschwindner

7. Silicon Photonic Micro-Ring Resonators for Drug Screening and Kinetic Analysis

Muzammil Iqbal, Rufus W. Burlingame, Randy Romero, Annabel Wang, Tyler Grove, and Martin A. Gleeson

8. Optical Waveguide Light-Mode Spectroscopy for Ion Channel Profiling

Inna Szekacs

Part III: Cell Phenotypic Profiling and Screening

9. Label-Free Profiling of Endogenous Receptor Responses in Primary Isolated Cardiac Cells

Douglas G. Tilley, Ashley A. Repas, and Rhonda L. Carter

10. Surface Plasmon Resonance to Study Cell Signaling and GPCR Functional Selectivity in Live Cells

Philippe Bourassa, Thomas Soellradl, Jean-Sebastien Maltais, Paul G. Charette, Louis Gendron, and Michel Grandbois

11. Triple-Addition Label-Free Assays for High Throughput Screening of Muscarinic M1 Receptor Agonists, Antagonists, and Allosteric Modulators

Hannah J. Gitschier, Audrey B. Bergeron, David H. Randle, Caryn E. Bacon, Melvyn Baez, Peiyi Yang, Lisa M. Broad, Paul J. Goldsmith, Christian C. Felder, and Douglas A. Schober

12. Multiplexing Label-Free and Florescence-Based Methods for Pharmacological Characterization of GPCR Ligands

Huailing Zhong, Xinyan Huang, and Dario Doller

13. Label-Free Cell Phenotypic Identification of Active Compounds in Traditional Chinese Medicines

Xinmiao Liang, Jixia Wang, Xiuli Zhang, and Ye Fang

14. Use of the Quartz Crystal Microbalance with Dissipation Monitoring for Pharmacological Evaluation of Cell Signaling Pathways Mediated by Epidermal Growth Factor Receptors

Jennifer Y. Chen, Marcela P. Garcia, Lynn S. Penn, and Jun Xi

15. Profiling Sodium-Dependent Phosphate Transporter NaPi-IIb with Resonant Waveguide Grating Biosensor

Soo-Hang Wong, Alice Gao, and Paul H. Lee

16. Label-Free Impedance Measurements for Profiling Drug-Induced Cardiotoxicity

Filomain Nguemo, Judith Semmler, and Jurgen Hescheler

17. Digital Holographic Imaging for Label-Free Phenotypic Profiling, Cytotoxicity, and Chloride Channels Target Screening

Benjamin Rappaz, Fabien Kuttler, Billy Breton, and Gerardo Turcatti

18. Label-Free Profiling of Cell Adhesion: Determination of the Dissociation Constant for Native Cell Membrane Adhesion Receptor-Ligand Interaction

Norbert Orgovan, Beatrix Peter, Szilvia Bosze, Jeremy J. Ramsden, Balint Szabo, and Robert Horvath

19. Label-Free Impedance-Based Monitoring of Cell Migration and Invasion

Ridha Limame and Olivier De Wever

20. Infrared Surface Plasmon Spectroscopy Decodes Early Processes in Epithelial Host Cells Upon Enteropathogenic Escherichia coli Infection

Victor Yashunsky and Benjamin Aroeti

21. Surface Plasmon Resonance for Clinical Diagnosis of Type I Allergy

Yuhki Yanase and Michihiro Hide