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Kidney Research
Experimental Protocols
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Main description:

As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney.


Contents:

Part 1: Isolation and Propagation of Cell Populations

Isolation and Propagation of Glomerular Mesangial Cells

Paolo Mene and Antonella Stoppacciaro

Isolation and Primary Culture of Human Proximal Tubule Cells

David A. Vesey, Weier Qi, Xinming Chen, Carol A. Pollock and David W. Johnson

Propagation and Culture of Renal Fibroblasts

Lauren Grimwood and Rosemary Masterson
Part 2: Animal Models

Small Animal Models of Kidney Disease: A Review

Tim D. Hewitson, Takahiko Ono and Gavin J. Becker

Part 3: Techniques and Applications

Measurement of Glomerular Filtration Rate in Conscious Mice

Zhonghua Qi and Matthew D. Breyer

Laser Capture Microdissection of Kidney Tissue

Robert P. Woroniecki and Erwin P. Bottinger

Quantitative Gene Expression Analysis in Kidney Tissues

Chris Tikellis, Philip Koh, Wendy Burns and Phillip Kantharidis

In vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy

A. Richard Kitching, Michael P. Kuligowski and Michael J. Hickey

Using In situ Hybridisation to Localize Renal Gene Expression in Tissue Sections

Ian A. Darby, Alexis Desmouliere & Tim D. Hewitson

Immuno and Lectin Histochemistry for Renal Light Microscopy

Tim D. Hewitson and Lauren Grimwood

Immuno and Lectin Histochemistry for Renal Electron Microscopy

Mitsuru Nakajima

Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia

Christian Rosenberger, Seymour Rosen, Alexander Paliege and Samuel N. Heyman

Identification of Apoptosis in Kidney Tissue Sections

Glenda Gobe

Cell Populated Floating Collagen Lattices: An in vitro Model of Parenchymal Contraction

Kristen J. Kelynack

Mechanical Stretch-induced Signal Transduction in Cultured Mesangial Cells

Joan Krepinsky

Determination of Collagen Content, Concentration and Sub-types in Kidney Tissue

Chrishan S. Samuel

SELDI-TOF Mass Spectrometry-based Protein Profiling of Kidney Tissue

Eleni Giannakis, Chrishan S. Samuel, Wee-Ming Boon, Mary Macris, Tim D. Hewitson and John D. Wade

In vivo Transfer of Small Interfering RNA or Small Hairpin RNA Targeting Glomeruli

Yoshitsugu Takabatake, Yoshitaka Isaka and Enyu Imai


PRODUCT DETAILS

ISBN-13: 9781493956401
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2016
Pages: None
Weight: 607g
Availability: Available
Subcategories: Biochemistry, General Issues, Nephrology, Pathology

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