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MORE ABOUT THIS BOOK
Main description:
As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney.
Contents:
Part 1: Isolation and Propagation of Cell Populations
Isolation and Propagation of Glomerular Mesangial Cells
Paolo Mene and Antonella Stoppacciaro
Isolation and Primary Culture of Human Proximal Tubule Cells
David A. Vesey, Weier Qi, Xinming Chen, Carol A. Pollock and David W. Johnson
Propagation and Culture of Renal Fibroblasts
Lauren Grimwood and Rosemary Masterson
Part 2: Animal Models
Small Animal Models of Kidney Disease: A Review
Tim D. Hewitson, Takahiko Ono and Gavin J. Becker
Part 3: Techniques and Applications
Measurement of Glomerular Filtration Rate in Conscious Mice
Zhonghua Qi and Matthew D. Breyer
Laser Capture Microdissection of Kidney Tissue
Robert P. Woroniecki and Erwin P. Bottinger
Quantitative Gene Expression Analysis in Kidney Tissues
Chris Tikellis, Philip Koh, Wendy Burns and Phillip Kantharidis
In vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy
A. Richard Kitching, Michael P. Kuligowski and Michael J. Hickey
Using In situ Hybridisation to Localize Renal Gene Expression in Tissue Sections
Ian A. Darby, Alexis Desmouliere & Tim D. Hewitson
Immuno and Lectin Histochemistry for Renal Light Microscopy
Tim D. Hewitson and Lauren Grimwood
Immuno and Lectin Histochemistry for Renal Electron Microscopy
Mitsuru Nakajima
Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia
Christian Rosenberger, Seymour Rosen, Alexander Paliege and Samuel N. Heyman
Identification of Apoptosis in Kidney Tissue Sections
Glenda Gobe
Cell Populated Floating Collagen Lattices: An in vitro Model of Parenchymal Contraction
Kristen J. Kelynack
Mechanical Stretch-induced Signal Transduction in Cultured Mesangial Cells
Joan Krepinsky
Determination of Collagen Content, Concentration and Sub-types in Kidney Tissue
Chrishan S. Samuel
SELDI-TOF Mass Spectrometry-based Protein Profiling of Kidney Tissue
Eleni Giannakis, Chrishan S. Samuel, Wee-Ming Boon, Mary Macris, Tim D. Hewitson and John D. Wade
In vivo Transfer of Small Interfering RNA or Small Hairpin RNA Targeting Glomeruli
Yoshitsugu Takabatake, Yoshitaka Isaka and Enyu Imai
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2016
Pages: None
Weight: 607g
Availability: Available
Subcategories: Biochemistry, General Issues, Nephrology, Pathology