Main description:

Vitrification in Assisted Reproduction presents standard and new cryopreservation techniques in detail, outlining those that have resulted in success, and providing recommended means for overcoming typically encountered problems.

This new edition provides a much broader range of clinical application and data to demonstrate its contribution to the use of vitrified oocytes and embryos. The book also discusses new areas in the field of assisted reproductive technology such as oocyte banking, preimplantion diagnostics at the blastocyst stage, and the burgeoning adoption of elective single embryo transfer.

Written by expert scientists and clinical embryologists, this book will help you to consistently and predictably apply vitrification as an important therapeutic strategy in assisted reproduction.

Contents:

Overview of Biological Vitrification. Vitrification of Oocytes and Embryos: Finally a Recognized Technique, But Still a Source of Concern and Debate. Intracellular Concentration of Cryoprotectant During Vitrification and Slow-Freezing Cryopreservation Procedures? Importance of Cooling Versus Warming Rates in Vitrification Techniques. The Movement of Water and Cryoprotectants in Mammalian Oocytes and Embryos: Membrane Permeability and Aquaporins. Open Versus Closed Systems. Automatic Vitrification: Development of the Gavi System. Vitrification at Minimum Volume: From Basic Science to Clinical Application. Vitrification of Oocytes: General Considerations and the Use of the Cryotec Method. Safety of Vitrification and Cryostorage and Optimization of Cryopreservation Protocols. Physiological Aspects of Oocyte Vitrification. Vitrification of Oocytes: Imprinting and Disturbance in Spindle Formation and Chromosome Segregation. Metabolic Profile of Day 3 Embryos Arising from Vitrified Oocytes. Vitrification of Human Oocytes for In Vitro Fertilization Patients. Oocyte Vitrification: Donor "Egg Banking." Fertility Preservation for oncology Patients. Vitrification of Human Ovarian Tissue. Vitrification of Cleavage-Stage Embryos and Blastocysts and Their Neonatal Outcomes. Vitrification of Human Blastocysts: Clinical Realities and Neonatal Outcomes. Development and Hatching of Human Blastocysts After Vitrification and Warming. Does Storage of Vitrified Blastocysts Have an Impact on Implantation Potential and Birth Rate? Ovarian Tissue Vitrification: Clinical Realities and Outcomes Chapter for From Basic Science to Clinical Application. Vitrification of Human Testicular Tissue, Spermatogonia, and Spermatozoa. Vitrification in Pluripotent Stem Cell Banking: Requirements and Technical Solutions For Large-Scale Biobanks. Scrying The Future: The ongoing Transformation of Reproductive Medicine Through Vitrification.