Main description:

This detailed book explores protocols with the aim of testing aerobic exercise, resistance training, special diets, additives and natural products, which have led to new insights into the physiological and molecular aspects of health and disease. Many of these approaches have contributed to significant improvements in disease areas such as cardiovascular disease, cognitive dysfunction, diabetes, frailty, gliobastoma, metabolic syndrome, obesity, oxidative stress, and various cancers. This collection also provides important information on disease mechanisms and novel drug targets as each protocol is presented in the context of specific chronic diseases or different therapeutic areas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and reflective of research from all around the globe, Physical Exercise and Natural and Synthetic Products in Health and Disease serves as an ideal guide for researchers in the areas of chronic disease, exercise, and nutrition, as well as to clinical scientists, physicians, and pharmacologists as it gives insights into possibilities for the development of novel therapeutics and the means of monitoring therapeutic response through the measurement of molecular and physiometric biomarkers.

Contents:

Part I: Reviews

1. The New Frontier of Three-Dimensional Culture Models to Scale Up Cancer Research

Caleb Jensen, Chloe Shay, and Yong Teng

2. Therapeutic Effects of Resveratrol on Non-Alcoholic Fatty Liver Disease through Inflammatory, Oxidative Stress, Metabolic, and Epigenetic Modifications

Mina Karimi, Behnaz Abiri, Paul C. Guest, and Mohammadreza Vafa

3. A Matter of Fat: Body Fat Distribution and Cardiometabolic Disease in Africa

Nasrin Goolam Mahyoodeen and Nigel J. Crowther

4. PGK1: An Essential Player in Modulating Tumor Metabolism

Leslie Duncan, Chloe Shay, and Yong Teng

5. The Association of Reproductive Aging with Cognitive Function in Sub-Saharan African Women

Nicole G. Jaff and Nigel J. Crowther

6. The Effects of Exercise on Lipid Biomarkers

Michael F. Mendoza, Sergey M. Kachur, and Carl J. Lavie

7. Exercise Training Protocols to Improve Obesity, Glucose Homeostasis, and Subclinical Inflammation

Tiego A. Diniz, Barbara M. Antunes, Jonathan P. Little, Fabio S. Lira, and Jose Cesar Rosa-Neto

Part II: Protocols

8. A Protocol of Intradialytic Exercise for Improvements in Inflammatory Status, Body Composition, and Functional Capacity

Lorena Cristina Curado Lopes, Paula Alves Monteiro, and Joao Felipe Mota

9. Evaluating the Anticancer Activity of Natural Products Using a Novel 3D Culture Model

Chloe Shay and Yong Teng

10. Evaluation of Antidiabetic Properties of Adenosma bracteosum Bonati Extracts in Mice with Streptozotocin-Induced Diabetes

Giau Van Vo, Paul C. Guest, and Ngoc Hong Nguyen

11. Testing the Effects of Cinnamon Extract Supplementation on Inflammation and Oxidative Stress Induced by Acrylamide

Fatemeh Haidari, Majid Mohammadshahi, Behnaz Abiri, Paul C. Guest, Mehdi Zarei, and Mojdeh Fathi

12. Proteomic Mapping of the Human Myelin Proteome

Paul C. Guest

13. Testing for Thyroid Peroxidase and Anti-Neuronal Antibodies in Depression and Schizophrenia

Johann Steiner, Winfried Stoecker, Bianca Teegen, Henrik Dobrowolny, Gabriela Meyer-Lotz, Katrin Borucki, Paul C. Guest, and Hans-Gert Bernstein

14. Evaluation of Antimicrobial and Anticancer Activities of Bouea macrophylla Ethanol Extract

Giau Van Vo, Paul C. Guest, and Ngoc Hong Nguyen

15. Evaluation of Anti-Hepatocellular-Cancer Properties of -Sitosterol and -Sitosterol-Glucoside from Indigofera zollingeriana Miq

Giau Van Vo, Paul C. Guest, Thuy Trang Nguyen, and Tuong Kha Vo

16. Label-Free Electrochemical Biosensors to Evaluate the Antioxidant Effect of Tocopherol in Ultraviolet Radiation

Lixia Gao and Yong Teng

17. qRT-PCR Analysis of GLUT-4 and Assessment of Trolox as an Effective Antioxidant in Diabetic Cardiomyoblasts

S. Asha Devi, Ravichandra Shivalingappa Davargaon, and Subramanyam Muthangi VV

18. Flow Cytometric Analysis of Hyperglycemia-Induced Cell Death Pathways in Cardiomyoblasts

Ravichandra Shivalingappa Davargaon, Subramanyam Muthangi VV, and S. Asha Devi

19. Vietnamese Medicinal Plants as Potential Resources to Explore New Anti-Cancer and Anti-Inflammation: Established Assays for Pharmacological Tests

Trang Thi Phuong Nguyen, Dieu Thi Xuan Nguyen, and Triet Thanh Nguyen

20. Testing the Effect of Curcumin on Proliferative Capacity of Colorectal Cancer Cells

Tannaz Jamialahmadi, Paul C. Guest, Amir R. Afshari, Muhammed Majeed, and Amirhossein Sahebkar

21. Assessment of Topical and Transdermal Penetration of Curcuma heyneana Rhizome Extract in Rat Skin: Histological Analysis

Idha Kusumawati, Rohmania, Mega Ferdina Warsito, and Eka Pramyrtha Hestianah

22. Measuring the Effects of Berberine on Serum Pro-Oxidant-Antioxidant Balance in Metabolic Syndrome

Tannaz Jamialahmadi, Paul C. Guest, Aida Tasbandi, Khalid Al-Rasadi, and Amirhossein Sahebkar

23. Testing the Anti-Inflammatory Effects of Curcuminoids in Patients with Colorectal Cancer

Tannaz Jamialahmadi, Paul C. Guest, Aida Tasbandi, Muhammed Majeed, and Amirhossein Sahebkar

24. Analytical Methods and Bioassays for Cytotoxicity and Anti-Diabetic Properties of Aquilaria Crassna Leaf Extracts in HepG2 Cells

Pinnara Rojvirat, Netiya Karaket, Phanupol Mongkolsiri, and Sarawut Jitrapakdee

25. Testing the Physical and Molecular Effects of Nutritional Supplements and Resistance Exercise in Middle-Aged Females

Behnaz Abiri, Paul C. Guest, Parvin Sarbakhsh, and Mohammadreza Vafa

26. Analysis of Cytotoxic Effects of Zerumbone in Malignant Glioblastoma Cells

Mohammad Jalili-Nik, Amir R. Afshari, Khadijeh Mahboobnia, Paul C. Guest, Tannaz Jamialahmadi, and Amirhossein Sahebkar

27. Protocol for Testing the Potential Antioxidant Effects of Curcuminoids on Patients with Type 2 Diabetes Mellitus

Tannaz Jamialahmadi, Yunes Panahi, Muhammed Majeed, Paul C. Guest, and Amirhossein Sahebkar

28. The Detection of Toxic Compounds in Extracts of Callilepis laureola (Ox-Eye Daisy) and Senecio latifolius (Ragwort) by Ultra-Performance Liquid Chromatography Mass Spectrometry (UPLC-MS/MS)

Tracy Snyman and Nigel J. Crowther

29. Impact of Curcumin on Hepatic Low-Density Lipoprotein Uptake

Mohammad Jalili-Nik, Khadijeh Mahboobnia, Paul C. Guest, Muhammed Majeed, Khalid Al-Rasadi, Tannaz Jamialahmadi, and Amirhossein Sahebkar