Main description:

This collection explores state-of-the-art methods and protocols for research on photodynamic therapy (PDT) and its use in a wide range of medical applications, from antiviral to anticancer. Beginning with an extensive section on in vitro and in vivo models, the volume continues with chapters on oxygen-independent photosensitizers, next-generation photosensitization strategies, contemporary insights into the immunomodulatory effects of PDT, antimicrobial effects of PDT, as well as a variety of general biochemical and molecular biological techniques. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that ensures successful results in the lab.
Thorough and authoritative, Photodynamic Therapy: Methods and Protocols serves as an ideal source of inspiration for both new and established PDT scientists and a guide for designing innovative research programs in this continuously advancing and multidisciplinary field.

Contents:

Part I: In Vitro and In Vivo Models to Study Photodynamic Therapy

1. Application of Monolayer Cell Cultures for Investigating Basic Mechanisms of Photodynamic Therapy

Mans Broekgaarden

2. The Negative Impact of Cancer Cell Nitric Oxide on Photodynamic Therapy

Jonathan M. Fahey and Albert W. Girotti

3. Microtumor Models as a Preclinical Investigational Platform for Photodynamic Therapy

Mans Broekgaarden and Jean-Luc Coll

4. A Microfluidic Model to Evaluate Photodynamic Therapy Treatment Response in 3D Tumors

Shubhankar Nath, Michael Pigula, Tayyaba Hasan, and Imran Rizvi

5. Analysis of Treatment Effects on Structurally Complex Microtumor Cultures Using a Comprehensive Image Analysis Procedure

Anne-Laure Bulin, Mans Broekgaarden, and Tayyaba Hasan

6. High-Throughput Examination of Therapy-Induced Alterations in Redox Metabolism in Spheroid and Microtumor Models

Mans Broekgaarden, Anne-Laure Bulin, and Tayyaba Hasan

7. Spatiotemporal Tracking of Different Cell Populations in Cancer Organoid Models for Investigations on Photodynamic Therapy

Anne-Laure Bulin and Tayyaba Hasan

8. Generating Large Numbers of Pancreatic Microtumors on Alginate-Gelatin Hydrogels for Quantitative Imaging of Tumor Growth and Photodynamic Therapy Optimization

Nazareth Milagros Carigga Gutierrez, Tristan Le Clainche, Jean-Luc Coll, Lucie Sancey, and Mans Broekgaarden

9. The Chicken Embryo Chorioallantoic Membrane as an In Vivo Model for Photodynamic Therapy

Jaroslava Joniova and Georges Wagnieres

10. Subcutaneous Xenograft Models for Studying PDT In Vivo

Girgis Obaid and Tayyaba Hasan

11. In Vivo Models for Studying Interstitial Photodynamic Therapy of Locally Advanced Cancer

Gal Shafirstein, Emily Oakley, Sasheen Hamilton, Michael Habitzruther, Sarah Chamberlain, Sandra Sexton, Leslie Curtin, and David A. Bellnier

12. Orthotopic Models of Pancreatic Cancer to Study PDT

Girgis Obaid, Zhiming Mai, and Tayyaba Hasan

13. An Orthotopic Murine Model of Peritoneal Carcinomatosis of Ovarian Origin for Intraoperative PDT

Thierry Michy, Claire Bernard, Jean Luc Coll, and Veronique Josserand

14. Photodynamic Treatments for Disseminated Cancer Metastases Using Fiber-Optic Technologies

Eric M. Kercher and Bryan Q. Spring

15. Stereotaxic Implantation of F98 Cells in Fischer Rats: A Syngeneic Model to Investigate Photodynamic Therapy Response in Glioma

Anne-Laure Bulin, Jean-Francois Adam, and Helene Elleaume

Part II: Inorganic Photosenstizers and Light-Activatable Anti-Cancer Prodrugs

16. New Generation of Photosensitizers Based on Inorganic Nanomaterials

Xiaomin Liu and Hong Zhang

17. Cytotoxicity of Metal-Based Photoactivated Chemotherapy (PACT) Compounds

Vadde Ramu, Austin B. Auyeung, and Sylvestre Bonnet

Part III: Third and Fourth-Generation Photosensitizers

18. Photosensitized Oxidation of Intracellular Targets: Understanding the Mechanisms to Improve the Efficiency of Photodynamic Therapy

Thiago Teixeira Tasso and Mauricio da Silva Baptista

19. Inhibition of the HIF-1 Survival Pathway as a Strategy to Augment Photodynamic Therapy Efficacy

Mark J. de Keijzer, Daniel J. de Klerk, Lianne R. de Haan, Robert T. van Kooten, Leonardo P. Franchi, Lionel M. Dias, Tony G. Kleijn, Diederick J. van Doorn, and Michal Heger on behalf of the International Photodynamic Therapy Study Group

20. Strategies for Improving Photodynamic Therapy through Pharmacological Modulation of the Immediate Early Stress Response

Daniel J. de Klerk, Mark J. de Keijzer, Lionel M. Dias, Jordi Heemskerk, Lianne R. de Haan, Tony G. Kleijn, Leonardo P. Franchi, and Michal Heger on behalf of the International Photodynamic Therapy Study Group

21. Nanobody-Targeted Photodynamic Therapy: Nanobody Production and Purification

Vida Mashayekhi, Erik Schooten, Paul M.P. van Bergen en Henegouwen, Marta M. Kijanka, and Sabrina Oliveira

22. Conjugation of IRDye Photosensitizers or Fluorophores to Nanobodies

Vida Mashayekhi and Sabrina Oliveira

23. In Vitro Assessment of Binding Affinity, Selectivity, Uptake, Intracellular Degradation, and Toxicity of Nanobody-Photosensitizer Conjugates

Irati Beltran Hernandez, Timo W.M. De Groof, Raimond Heukers, and Sabrina Oliveira

24. Assessment of the In Vivo Response to Nanobody-Targeted PDT through Intravital Microscopy

Henriette S. de Bruijn, Ann L.B. Seynhaeve, Timo L.M. ten Hagen, Sabrina Oliveira, and Dominic J. Robinson

25. Orthotopic Breast Cancer Model to Investigate the Therapeutic Efficacy of Nanobody-Targeted Photodynamic Therapy

Marion M. Deken, Shadhvi S. Bhairosingh, Alexander L. Vahrmeijer, and Sabrina Oliveira

26. Investigation of the Therapeutic Potential of Nanobody-Targeted Photodynamic Therapy in an Orthotopic Head and Neck Cancer Model

Pieter B.A.A. van Driel, Stijn Keereweer, Clemens W.G.M. Lowik, and Sabrina Oliveira

Part IV: Photodynamic Therapy-Induced Immune Signaling

27. Evaluation of the Antitumor Immune Response Following Photofrin-Based PDT in Combination with the Epigenetic Agent 5-Aza-2'-Deoxycytidine

Malgorzata Wachowska, Angelika Muchowicz, and Jakub Golab

28. Controlling Immunoregulatory Cell Activity for Effective Photodynamic Therapy of Cancer

Mladen Korbelik, Zdzislaw M. Szulc, Alicja Bielawska, and Duska Separovic

29. Measuring the Anti-Tumor T-Cell Response in the Context of Photodynamic Therapy

Jan Willem Kleinovink and Ferry Ossendorp

30. Combination of Photodynamic Therapy and Immune Checkpoint Blockade

Jan Willem Kleinovink and Ferry Ossendorp

31. Combination of Photodynamic Therapy and Therapeutic Vaccination

Jan Willem Kleinovink and Ferry Ossendorp

Part V: Antimicrobial Photodynamic Therapy

32. In Vitro Potentiation of Antimicrobial Photodynamic Inactivation by Addition of Potassium Iodide

Nasim Kashef and Michael R. Hamblin

33. In Vivo Potentiation of Antimicrobial Photodynamic Therapy in a Mouse Model of Fungal Infection by Addition of Potassium Iodide

Nasim Kashef and Michael R. Hamblin

34. Bioluminescent Models to Evaluate the Efficiency of Light-Based Antibacterial Approaches

Ana T.P.C. Gomes, Maria A.F. Faustino, Maria G.P.M.S. Neves, and Adelaide Almeida

35. Photochemical Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents against Intracellular Infections

Xiaolin Zhang, Leonie de Boer, and Sebastian. A. J. Zaat

36. Determination of the Efficiency of Photodynamic Decontamination of Food

Michael Glueck and Kristjan Plaetzer

Part VI: Molecular Techniques and Tools in Photodynamic Therapy Research

37. Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy

Enzo M. Scutigliani, Jakub A. Kochan, Emilie C.B. Desclos, Art Jonker, Michal Heger, and Przemek M. Krawczyk

38. Detection of Paraptosis after Photodynamic Therapy

David Kessel

39. Optimal Use of 2,7-Dichlorofluorescein Diacetate in Cultured Hepatocytes

Megan J. Reiniers, Lianne R. de Haan, Laurens F. Reeskamp, Mans Broekgaarden, Ruurdtje Hoekstra, Rowan F. van Golen, and Michal Heger