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Nucleic Acids Hybridization
Modern Applications
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Main description:

Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. Coverage includes experimental protocols along with comprehensive and detailed method descriptions.


Feature:

In-depth review of nucleic acid hybridization in vitro techniques


All major applications covered


Includes novel methods with the potential of being widely used


Detailed experimental protocols provided


Contents:

Contributing Authors
Preface
Acknowledgements Nucleic acids hybridization: potentials and limitations
Anton Buzdin Selective suppression of polymerase chain reaction and its most popular applications
Sergey Lukyanov, Konstantin Lukyanov, Nadezhda Gurskaya, Ekaterina Bogdanova, Anton Buzdin Suppression subtractive hybridization
Sergey Lukyanov, Denis Rebrikov, Anton Buzdin Stem-loop oligonucleotides as hybridization probes and their practical use in molecular biology and biomedicine
Anton Buzdin, Sergey Lukyanov Generation of normalized cDNA libraries
Alex Shcheglov, Pavel Zhulidov, Ekaterina Bogdanova, Dmitry Shagin Primer extension enrichment reaction (PEER) and other methods for difference screening
Lilia Ganova-Raeva Subtractive hybridization with covalently modified oligonucleotides
Shi-Lung Lin, Donald Chang, Joseph Miller and Shao-Yao Ying Coincidence cloning: robust technique for isolation of common sequences
Anton Buzdin DNA hybridization in solution for mutation detection
Anton Buzdin Current attempts to improve the specificity of nucleic acids hybridization
Anton Buzdin Concepts on microarray design for genome and transcriptome analyses
Helder I. Nakaya, Eduardo M. Reis and Sergio Verjovski-Almeida Index


PRODUCT DETAILS

ISBN-13: 9781402060397
Publisher: Springer (Springer Netherlands)
Publication date: June, 2007
Pages: 314
Weight: 666g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry, Genetics, Microbiology, Oncology
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