Main description:

This book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfalls of western blotting. The book also introduces simple protocols that can transform western blotting into a fun method, such as sending secret messages on membranes or using nitrocellulose membrane as a canvas for art.

In addition to the techniques, this book also covers the history of western blotting, which originated from the development of the blotting of DNA. It then delves into the importance of protein blotting, brought to the fore by the fact that the procedure has been evolving constantly since its inception in 1979, and the fact that the scientific community is faced with a multitude of ways and means of transferring proteins to membranes..

Contents:

Western blotting: How it began
Basics of Western Blotting

Basic Calculations in Western Blotting
Choosing The Right Antibody For Western Blotting

Sample Preparation

Blocking membranes at places that do not have transferred proteins

A Basic Method for Western Blotting

Trouble Shooting Western Blotting

Using Biotin to demonstrate western and dot blotting in University Practical Classes-

A Guide To Instructors

Nitrocellulose Membrane Art

The Use Of A Harmless Invisible Ink To Send Secret Messages On Nitrocellulose Membranes And As A Substitute For The Harmful Radioactive Ink Used As A Marker In ECL Membrane Assays

Fingerprinting On Nitrocellulose And Polyvinylidene Difluoride Membrane

13. 13. Spectrometric Determination Of Protein For Electrophoresis

14. 14. Concentrating Proteins For Western Blotting

15. 15. Dissolving Proteins Using Lysis Buffer

Blotting And Detecting Glycosaminoglycans

Passive Blotting From Tissue Slices

Localizing Proteins by Tissue Printing

Ultrasound Blotting

Obtaining Several Blots From A Single SDS-PAGE Gel By Passive Transfer

Diffusion Blotting From SDS-PAGE Gels Supported By Solid Plastic

Dot-Immunobinding

Simultaneous Immunoblotting Analysis With Activity Gel Electrophoresis

Protein Blotting By Centrifugation

Blotting And Immunostaining Of Multiple Antigenic Peptides

High Molecular Weight Protein Blotting Using Agarose Gel Electrophoresis

Cetyltrimethylammonium bromide PAGE And Eastern Blotting

Purification Of Autoantibodies Bound To An Autoantigen Immobilized On A Membrane Strip

Miniaturized Blotting

Affinity Immunoblotting

Transferring Coomassie Blue Stained Proteins from Polyacrylamide Gels To Transparencies

Protein Transfer From Immobilized pH Gradient Gels

SDS PAGE To Immunoblotting In Sixty Minutes

Western Blot Analysis Of Protein-DNA Complexes Formed DuringGel Shift Experiments

Electrophoretic Transfer Of High And Low Molecular Weight Proteins To Membranes Using Heat

Grid-immunoblotting

Far-western Blotting

Native Electrophoresis And Western Blot Analysis

Efficient Electroblotting Of Low Molecular Weight Protein After Staining With Coomassie

Avoiding Non-specific Binding Of Secondary Antibodies In Immunoblotting By Double-blotting

A Brief Introduction To Other Protein Blotting Methods

Quantitative Computerized Western Blotting In Detail

43. Common Problems In Electrophoresis