Main description:

This volume provides detailed protocols for the identification and understanding of vasculogenic mimicry process in vitro and in vivo, in addition to protocols for microscopy and histology. Chapters guide readers through different materials, commercial and homemade scaffolds, Matrigel, cancer spheroids, 3D tissue constructs, vasculogenic processes, and mathematical model building. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Vasculogenic Mimicry: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Contents:

PART I - Vasculogenic Mimicry Background

1. Vasculogenic Mimicry - An Overview

Emily Marques dos Reis and Fernanda Vieira Berti

2. In vitro Models to Study Angiogenesis and Vasculature

Ralph Francescone and Debora Barbosa Vendramini-Costa

PART II: Protocols for VM in vitro

3. In vitro Tube Formation Assays in Matrigel

Ralph Francescone and Debora Barbosa Vendramini-Costa

4. Vasculogenic mimicry in a 3D model in vitro

Emily Marques dos Reis, Fernanda Vieira Berti, and Luismar Marques Porto

5. Cancer Cell Spheroids as a 3D Model for Exploring the Pathobiology of Vasculogenic Mimicry

Maira de Andrade Peixoto, Emily Marques dos Reis, and Luismar Marques Porto

6. A Three-Dimensional Culture-Based Assay to Detect Early Stages of Vasculogenic Mimicry in Ovarian Cancer Cells

Yarely M. Salinas-Vera, Dolores Gallardo-Rincon, Erika Ruiz-Garcia, Laurence A. Marchat, Jesus Valdez, Carlos Vazquez-Calzada, and Cesar Lopez-Camarillo

7. Co-Immunoprecipitation of Protein Complexes from Different Subcellular Compartments in Vasculogenic Mimicry Studies

Daniel Delgado-Bellido, Angel Garcia-Diaz, and Francisco Javier Oliver

PART III - Protocols for VM In Vivo

8. In vivo Models to Evaluate Antitumor Drugs Effect on Vasculogenic Mimicry

Carlos E. Fonseca-Alves and Patricia de F. Lainetti

9. Patient derived xenograft models for studying vascular mimicry in melanoma in vivo

Manish Ranjan and Nobel Bhasin

PART IV: VM Analysis

10. Histological evaluation of long-term collagen I culture

Marcella Massimini, Mariarita Romanucci, Raffaella De Maria, and Leonardo Della Salda

11. Histochemical Staining of Vasculogenic Mimicry

Saber Imani, Shuya Liu, Mazaher Maghsoudloo, and QingLian Wen

12. Identification of Vasculogenic Mimicry in Histological Samples

Gustav Stalhammar

13. Evaluation of Pharmaceutical Inhibition of Vasculogenic Mimicry in vitro

Joseph Manarang and Alison McDermott

14. Immunohistochemistry for VM markers

Alessio Biagioni and Elena Andreucci

15. Confocal laser microscopy for VM analysis with DAPI and phalloidin staining

Karina Cesca and Eliana Medeiros Oliveira

16. Building Mathematical Models for Vascular Growth and Inhibition

Fernanda Vieira Berti and Luismar Marques Porto