Main description:

This volume provides a comprehensive outline of the current methods used to detect, characterize, and investigate Shiga toxin-producing E. coli (STEC) and Shiga toxins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Shiga Toxin-Producing E. coli: Methods and Protocols aims to be a valuable resource for clinicians, epidemiologists, and researchers interested in STEC pathogenesis.

Contents:

1. Integrated Approach for the Diagnosis of Shiga Toxin-producing Escherichia coli Infections in Humans

Stefano Morabito, Fabio Minelli and Rosangela Tozzoli

2. Shiga Toxin-producing E. coli in Animals - Detection, Characterisation, and Virulence Assessment

Stefanie A. Barth, Rolf Bauerfeind, Christian Berens, and Christian Menge

3. Identification of Shiga Toxin-producing Escherichia coli Outbreaks using Whole Genome Sequencing

Stefan Bletz, Alexander Mellmann, and Barbara Middendorf-Bauchart

4. Predicting Host Association for Shiga Toxin-producing E. coli serogroups by Machine Learning

Nadejda Lupolova, Antonia Chalka, and David L. Gally

5. Isolation and Characterization of Shiga Toxin Bacteriophages

Lorena Rodriguez-Rubio and Maite Muniesa

6. Lambda Red Mediated Recombination in Shiga toxin-producing Escherichia coli

Kenneth G Campellone and Alyssa M Coulter

7. Functional Analysis of Shiga Toxin-producing Escherichia coli Biofilm Components in Plant Leaves

Nicola J. Holden, Kathryn M. Wright, Jacqueline Marshall, and Ashleigh Holmes

8. Virulence Factor Cargo and Host Cell Interactions of Shiga Toxin-producing Escherichia coli Outer Membrane Vesicles

Martina Bielaszewska, Lilo Greune, Andreas Bauwens, Petra Dersch, Alexander Mellmann, and Christian Ruter

9. Isolation and Characterization of Shiga Toxin-Associated Microvesicles

Annie Willysson, Anne-lie Stahl, and Diana Karpman

10. Thin-Layer Chromatography in Structure and Recognition Studies of Shiga Toxin Glycosphingolipid Receptors

Johanna Detzner, Gottfried Pohlentz, and Johannes Muthing

11. Identification of Nanobodies Blocking Intimate Adherence of Shiga Toxin-producing Escherichia coli to Epithelial Cells

David Ruano-Gallego and Luis Angel Fernandez

12. Determining Shiga Toxin-producing Escherichia coli Interactions with Human Intestinal Epithelium in a Microaerobic Vertical Diffusion Chamber

Conor J. McGrath and Stephanie Schuller

13. Human Epithelial Stem Cell-Derived Colonoid Monolayers as a Model to Study Shiga Toxin-Producing Escherichia coli-Host Interactions

Karol Dokladny, Julie G. In, James Kaper, and Olga Kovbasnjuk

14. Use of the Dynamic TIM-1 Model for an In-depth understanding of the Survival and Virulence Gene Expression of Shiga toxin-producing Escherichia coli in the Human Stomach and Small Intestine

Ophelie Uriot, Sandrine Chalancon, Carine Mazal, Lucie Etienne-Mesmin, Sylvain Denis, and Stephanie Blanquet-Diot

15. Measuring Effector-mediated Modulation of Inflammatory Responses to Infection with Enteropathogenic and Shiga toxin-producing E. coli

Georgina L. Pollock, Cristina Giogha, and Elizabeth L. Hartland

16. Interaction of Bovine Lymphocytes with Products of Shiga toxin-Producing Escherichia coli

Andrew G. Bease, Robin L. Cassady-Cain, and Mark P. Stevens

17. Infection of Immunocompetent Conventional Mice with Shiga toxin-producing E. coli: The DSS+STEC Model

Gregory Hall, Shinichiro Kurosawa, and D. J. Stearns-Kurosawa

18. Infant Rabbit Model for Studying Shiga Toxin-producing Escherichia coli

Jennifer M. Ritchie

19. Citrobacter rodentium lysogenized with a Shiga Toxin-Producing Phage: A Murine Model for Shiga Toxin-producing E. coli Infection

Laurice J. Flowers, Shenglan Hu , Anishma Shrestha, Amanda J. Martinot, John M. Leong, and Marcia S. Osburne

20. Overview of the Effect of Citrobacter rodentium Infection On Host Metabolism And The Microbiota

Eve G. D. Hopkins and Gad Frankel