Main description:

This detailed volume presents a variety of animal models that are commonly used to study sepsis and some key procedures to measure specific disease outcomes. The chapters describe well-established surgical and nonsurgical rodent models of sepsis, presented by experts in the field. In addition, the book includes protocols for burn injury and sepsis, modeling the mouse intensive care unit (MICU), and the development of humanized mice, which may be useful tools to increase the translational potential of rodent sepsis research. There is a chapter discussing the use of biomarkers for sepsis diagnosis and prognosis in humans and mice, as well as chapters describing specific methods for biomarker measurement, and the use of analgesics and humane endpoints in rodent sepsis research. Finally, agent-based computational modeling is presented as a valuable complementary approach to study sepsis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Sepsis: Methods and Protocols serves as an ideal guide for researchers working toward improving the identification and treatment of septic patients.

Contents:

1. Cecal Ligation and Puncture

Susanne Drechsler and Marcin Osuchowski

2. Colon Ascendens Stent Peritonitis (CASP)

Anna Herminghaus and Olaf Picker

3. Induction of Sepsis via Fibrin Clot Implantation

Sailaja Ghanta, Min-Young Kwon, and Mark A. Perrella

4. Cecal Slurry Injection in Neonatal and Adult Mice

Jaimar C. Rincon, Philip A. Efron, Lyle L. Moldawer, and Shawn D. Larson

5. Injection of Escherichia coli to Induce Sepsis

Xian-Hui He, Dong-Yun Ouyang, and Li-Hui Xu

6. A Mouse Model of Pseudomonas aeruginosa Pneumonia

Brian W. LeBlanc and Craig T. Lefort

7. A Mouse Model of Candidiasis

Pilar Fajardo, Ana Cuenda, and Juan Jose Sanz-Ezquerro

8. Francisella tularensis Infection of Mice as a Model of Sepsis

Charles T. Spencer, Mireya G. Ramos Muniz, Nicole R. Setzu, and Michelle A. Sanchez

9. A Mouse Model of Necrotizing Enterocolitis

Belgacem Mihi, Wyatt E. Lanik, Qingqing Gong, and Misty Good

10. A Murine Model of Full-Thickness Scald Burn Injury with Subsequent Wound and Systemic Bacterial Infection

Antonio Hernandez, Naeem K. Patil, and Julia K. Bohannon

11. Mouse Intensive Care Unit (MICU)

Tamara Merz, Sandra Kress, Michael Groeger, Peter Radermacher, and Oscar McCook

12. Creation of BLT Humanized Mice for Sepsis Studies

Erica L. Heipertz and Wendy E. Walker

13. Scoring Sepsis Severity in Mice

Tina S. Mele

14. Identification of ILC2 in the Lung Using Flow Cytometry

Hui Xu and Meihong Deng

15. Measurement of Intestinal Permeability During Sepsis

Takehiko Oami and Craig M. Coopersmith

16. Sepsis Biomarkers

Yachana Kataria and Daniel Remick

17. Detection of Blood Cell Surface Biomarkers in Septic Mice

Dinesh G. Goswami and Wendy E. Walker

18. Microfluidic Chips for Sepsis Diagnosis

Yun Zhou, Yijia Yang, and Dimitri Pappas

19. Analgesia and Humane Endpoints for Rodents in Sepsis Research

Christine A. Boehm and Jean A. Nemzek

20. Agent-Based Modeling of Systemic Inflammation: A Pathway towards Controlling Sepsis

Gary An and Chase Cockrell